Granted Patent B2

US 12,305,203 · App. 17/312,697 · Granted May 20, 2025

Inventors: Hyun June Park (Seoul, KR); Sang Young Yoon (Seoul, KR); Hyun Kug Cho (Seoul, KR); Sung Jae Yang (Seoul, KR); So-hyeong Kim (Seoul, KR); Seung Hwan Kim (Seoul, KR); Il Hyang Park (Seoul, KR); Byung-sam Son (Seoul, KR); Seong Bo Kim (Seoul, KR); Seung Won Park (Seoul, KR)

Assignee: CJ CHEILJEDANG CORPORATION

C12N9/16C12P19/02C12Y501/03

Abstract

Claims (21)

1. A method for producing psicose, comprising converting psicose-6-phosphate into psicose by bringing psicose-6-phosphate into contact with a psicose-6-phosphate phosphatase, a microorganism expressing the same, or a culture of the microorganism, wherein the psicose-6-phosphate phosphatase is derived from any one selected from the group consisting of the genus Alicyclobacillus, Amycolatopsis, Anaerolinea, Archaeoglobus, Bacillus, Caldicellulosiruptor, Caldilinea, Caldithrix, Carboxydocella, Carboxydothermus, Chloroflexi, Defluviitoga, Deinococcus, Desulfurococcus, Dictyoglomus, Effusibacillus, Fervidobacterium, Geobacillus, Halococcus, Hydrogenivirga, Hydrogenobacter, Hyperthermus, Kosmotoga, Marinitoga, Meiothermus, Mesotoga, Metallosphaera, Methanocella, Methanococcoides, Methanohalobium, Methanolobus, Methanosarcina, Methanothermus, Petrotoga, Picrophilus, Pseudonocardia, Pyrococcus, Pyrodictium, Rhodothermus, Slackia, Staphylothermus, Sulfolobus, Thermanaerothrix, Thermoanaerobacter, Thermoanaerobacterium, Thermobifida, Thermococcus, Thermocrinis, Thermoflexus, Thermotoga, Thermus , and Truepera.

2. The method of claim 1 , wherein the method further comprises converting fructose-6-phosphate into psicose-6-phosphate by bringing fructose-6-phosphate into contact with fructose-6-phosphate-3-epimerase, a microorganism expressing the same, or a culture of the microorganism, prior to the converting psicose-6-phosphate into psicose.

3. The method of claim 2 , wherein the method further comprises converting glucose-6-phosphate into fructose-6-phosphate by bringing glucose-6-phosphate into contact with glucose-6-phosphate-isomerase, a microorganism expressing the same, or a culture of the microorganism, prior to the converting fructose-6-phosphate into psicose-6-phosphate.

4. The method of claim 3 , wherein the method further comprises converting glucose-1-phosphate into glucose-6-phosphate by bringing glucose-1-phosphate into contact with phosphoglucomutase, a microorganism expressing the same, or a culture of the microorganism, prior to the converting glucose-6-phosphate into fructose-6-phosphate.

5. The method of claim 3 , wherein the method further comprises converting glucose into glucose-6-phosphate by bringing glucose into contact with polyphosphate glucokinase, a microorganism expressing the same, or a culture of the microorganism, and polyphosphate, prior to the converting glucose-6-phosphate into fructose-6-phosphate.

6. The method of claim 4 , wherein the method further comprises converting starch, maltodextrin, sucrose, or a combination thereof into glucose-1-phosphate by bringing starch, maltodextrin, sucrose, or a combination thereof into contact with α-glucan phosphorylase, starch phosphorylase, maltodextrin phosphorylase, or sucrose phosphorylase; a microorganism expressing the same; or a culture of the microorganism, and phosphate, prior to the converting glucose-1-phosphate into glucose-6-phosphate.

7. The method of claim 6 , wherein the converting starch, maltodextrin, sucrose, or a combination thereof into glucose-1-phosphate further comprises converting starch, maltodextrin, sucrose, or a combination thereof into a maltooligosaccharide or glucose by further comprising α-amylase, pullulanase, isoamylase, α-glucanotransferase, glucoamylase, or sucrase; a microorganism expressing the same; or a culture of the microorganism.

8. A method for producing psicose, comprising bringing starch, maltodextrin, sucrose, or a combination thereof into contact with (a) a psicose-6-phosphate phosphatase; fructose-6-phosphate-3-epimerase; glucose-6-phosphate-isomerase; phosphoglucomutase or glucokinase; and α-glucan phosphorylase, starch phosphorylase, maltodextrin phosphorylase, sucrose phosphorylase, α-amylase, pullulanase, isoamylase, glucoamylase, α-glucanotransferase, polyphosphate glucokinase, or sucrase; or (b) a microorganism expressing the enzymes of item (a) or a culture of the microorganism, wherein the psicose-6-phosphate phosphatase is derived from any one selected from the group consisting of the genus Alicyclobacillus, Amycolatopsis, Anaerolinea, Archaeoglobus, Bacillus, Caldicellulosiruptor, Caldilinea, Caldithrix, Carboxydocella, Carboxydothermus, Chloroflexi, Defluviitoga, Deinococcus, Desulfurococcus, Dictyoglomus, Effusibacillus, Fervidobacterium, Geobacillus, Halococcus, Hydrogenivirga, Hydrogenobacter, Hyperthermus, Kosmotoga, Marinitoga, Meiothermus, Mesotoga, Metallosphaera, Methanocella, Methanococcoides, Methanohalobium, Methanolobus, Methanosarcina, Methanothermus, Petrotoga, Picrophilus, Pseudonocardia, Pyrococcus, Pyrodictium, Rhodothermus, Slackia, Staphylothermus, Sulfolobus, Thermanaerothrix, Thermoanaerobacter, Thermoanaerobacterium, Thermobifida, Thermococcus, Thermocrinis, Thermoflexus, Thermotoga, Thermus , and Trueper.

9. The method of claim 2 , the contact is performed at a pH of 5.0 to 9.0, at a temperature of 40° C. to 80° C., and/or for 2 hours to 24 hours or 120 hours.

10. The method of claim 1 , wherein the psicose-6-phosphate phosphatase is derived from any one selected from the group consisting of Alicyclobacillus acidocaldarius, Alicyclobacillus tengchongensis, Amycolatopsis thermoflava, Anaerolinea thermolimosa, Anaerolinea thermophila, Archaeoglobus fugidus, Archaeoglobus profundus, Archaeoglobus veneficus, Bacillus licheniformis, Caldicellulosiruptor bescii, Caldilinea aerophila, Caldithrix abyssi, Carboxydocella sp. ULO1, Carboxydothermus ferrireducens, Chloroflexi bacterium 54-19 , Defluviitoga tunisiensis, Deinococcus aerius, Deinococcus apachensis, Deinococcus aquatilis, Deinococcus geothermalis, Deinococcus hopiensis, Deinococcus maricopensis, Deinococcus murrayi, Deinococcus reticulitermitis, Deinococcus wulumuqiensis, Deinococcus sp. Leaf326 , Deinococcus phoenicis, Deinococcus proteolyticus, Deinococcus sp. 17bor-2 , Deinococcus sp. NW-56 , Deinococcus sp. RL, Deinococcus sp. YIM 77859 , Desulfurococcus mucosus, Dictyoglomus turgidum, Effusibacillus pohliae, Fervidobacterium gondwanense, Fervidobacterium islandicum, Fervidobacterium nodosum, Fervidobacterium pennivorans, Geobacillus sp., Geobacillus stearothermophilus, Halococcus salifodinae, Hydrogenivirga sp. 128-5-R1-1, Hydrogenobacter hydrogenophilus, Hydrogenobacter thermophilus, Hyperthermus butylicus, Kosmotoga arenicorallina, Kosmotoga olearia, Marinitoga piezophila, Meiothermus cerbereus, Meiothermus chliarophilus, Meiothermus ruber, Meiothermus silvanus, Meiothermus taiwanensis, Meiothermus timidus, Meiothermus rufus, Mesotoga infera, Metallosphaera sedula, Methanocella conradii, Methanococcoides methylutens, Methanohalobium evestigatum, Methanolobus tindarius, Methanosarcina sicilia, Methanothermus fervidus, Petrotoga mobilis, Picrophilus torridus, Pseudonocardia thermophila, Pyrococcus furiosus, Pyrodictium occultum, Rhodothermus marinus, Slackia heliotrinireducens, Staphylothermus marinus, Sulfolobus acidocaldarius, Thermanaerothrix daxensis, Thermoanaerobacter sp., Thermoanaerobacter thermohydrosulfuricus, Thermoanaerobacter wiegelii, Thermoanaerobacterium xylanolyticum, Thermobifida halotolerans, Thermococcus celer, Thermococcus litoralis, Thermococcus profundus, Thermocrinis minervae, Thermocrinis ruber, Thermoflexus hugenholtzii, Thermotoga lettingae, Thermotoga neapolitana, Thermotoga petrophilia, Thermus amyloliquefaciens, Thermus filiformis, Thermus thermophilus , and Truepera radiovictrix.

11. The method of claim 1 , wherein the psicose-6-phosphate phosphatase comprises an amino acid sequence having a homology of at least 70% to any one of amino acid sequences of SEQ ID NOS: 1 to 222.

12. The method of claim 1 , wherein the psicose-6-phosphate phosphatase comprises an amino acid sequence having a sequence identity of at least 70% to any one of amino acid sequences of SEQ ID NOS: 1, 6, 9, 12, 26, 29, 38 to 43, 45 to 53, 56, 57, 59, 60, 64 to 66, 69, 70, 72, 76, 80, 81, 91 to 93, 95, 99 to 103, 113, 114, 116, 117, 131, 134, 136, 142, 145, 146, 148, 164, 167, 169, 172, 177, 184 to 187, 189, 191, 192, 211, 217, and 221.

13. The method of claim 1 , wherein the psicose-6-phosphate phosphatase exhibits an activity selective to psicose-6-phosphate.

14. The method of claim 1 , wherein the psicose-6-phosphate phosphatase comprises an amino acid sequence of any one of amino acid sequences of SEQ ID NOS: 1, 6, 9, 12, 26, 29, 38 to 43, 45 to 53, 56, 57, 59, 60, 64 to 66, 69, 70, 72, 76, 80, 81, 91 to 93, 95, 99 to 103, 113, 114, 116, 117, 131, 134, 136, 142, 145, 146, 148, 164, 167, 169, 172, 177, 184 to 187, 189, 191, 192, 211, 217, and 221.

15. The method of claim 8 , wherein the psicose-6-phosphate phosphatase comprises an amino acid sequence having a homology of at least 70% to any one of amino acid sequences of SEQ ID NOS: 1, 6, 9, 12 to 14, 17, 18, 20 to 23, 25, 26, 28, 29, 37 to 53, 55 to 57, 59 to 61, 64 to 66, 69, 70, 72, 73, 76, 77, 80, 81, 85, 88, 91 to 93, 95, 99 to 105, 108, 113, 114, 116, 117, 122, 131, 133, 134, 136, 142 to 148, 150 to 152, 154 to 156, 159 to 164, 167, 169, 172, 173, 175, 177, 184 to 187, 189, 191, 192, 195, 198, 205, 210, 211, 214, 216, 217, and 221.

16. The method of claim 8 , wherein the psicose-6-phosphate phosphatase comprises an amino acid sequence of any one of SEQ ID NOS: 1, 6, 9, 12 to 14, 17, 18, 20 to 23, 25, 28, 29, 37 to 53, 55 to 57, 59 to 61, 64 to 66, 69, 70, 72, 73, 76, 77, 80, 81, 85, 88, 91 to 93, 95, 99 to 105, 108, 113, 114, 116, 117, 122, 131, 133, 134, 136, 142 to 148, 150 to 152, 154 to 156, 159 to 164, 167, 169, 172, 173, 175, 177, 184 to 187, 189, 191, 192, 195, 198, 205, 210, 211, 214, 216, 217, and 221.

17. The method of claim 8 , wherein the psicose-6-phosphate phosphatase comprises an amino acid sequence of any one of SEQ ID NOS: 1, 6, 9, 12, 29, 38 to 43, 45 to 53, 56, 57, 59, 60, 64 to 66, 69, 70, 72, 76, 80, 81, 91 to 93, 95, 99 to 103, 113, 114, 116, 117, 131, 134, 136, 142, 145, 146, 148, 164, 167, 169, 172, 177, 184 to 187, 189, 191, 192, 211, 217, and 221.

18. A method for producing psicose, comprising converting psicose-6-phosphate into psicose by bringing psicose-6-phosphate into contact with a psicose-6-phosphate phosphatase, a microorganism expressing the same, or a culture of the microorganism,

wherein the psicose-6-phosphate phosphatase comprises an amino acid sequence having a sequence identity of at least 70% to any one of amino acid sequences of SEQ ID NOS: 1, 6, 9, 12 to 14, 17, 18, 20 to 23, 25, 26, 28, 29, 37 to 53, 55 to 57, 59 to 61, 64 to 66, 69, 70, 72, 73, 76, 77, 80, 81, 85, 88, 91 to 93, 95, 99 to 105, 108, 113, 114, 116, 117, 122, 131, 133, 134, 136, 142 to 148, 150 to 152, 154 to 156, 159 to 164, 167, 169, 172, 173, 175, 177, 184 to 187, 189, 191, 192, 195, 198, 205, 210, 211, 214, 216, 217, and 221.

19. The method of claim 15 , wherein the psicose-6-phosphate phosphatase comprises an amino acid sequence of any one of SEQ ID NOS: 1, 6, 9, 12 to 14, 17, 18, 20 to 23, 25, 28, 29, 37 to 53, 55 to 57, 59 to 61, 64 to 66, 69, 70, 72, 73, 76, 77, 80, 81, 85, 88, 91 to 93, 95, 99 to 105, 108, 113, 114, 116, 117, 122, 131, 133, 134, 136, 142 to 148, 150 to 152, 154 to 156, 159 to 164, 167, 169, 172, 173, 175, 177, 184 to 187, 189, 191, 192, 195, 198, 205, 210, 211, 214, 216, 217, and 221.

20. The method of claim 15 , wherein the psicose-6-phosphate phosphatase comprises an amino acid sequence of any one of SEQ ID NOS: 1, 6, 9, 12, 29, 38 to 43, 45 to 53, 56, 57, 59, 60, 64 to 66, 69, 70, 72, 76, 80, 81, 91 to 93, 95, 99 to 103, 113, 114, 116, 117, 131, 134, 136, 142, 145, 146, 148, 164, 167, 169, 172, 177, 184 to 187, 189, 191, 192, 211, 217, and 221.

Assignments (1)

Priority Claims (1)

Continuity (1)

References Cited (31)