Deparaffinization of tissue by electric field generation and ionization
Paraffin-embedded tissue, which may be disposed on a solid substrate, is prepared by a dry technique that removes paraffin from tissue without adding any liquid to the tissue, thereby rendering the tissue substantially free of paraffin. The dry technique may entail applying heat energy to the tissue effective to melt the paraffin and thereby render it flowable, and applying an electric field. The electric field is effective to impart electrical charge to the paraffin and to move the paraffin out from the tissue due to electrical charge repulsion or attraction, which may be assisted by moving an electrode utilized to generate the electric field relative to the paraffin. The electric field, or both the electric field and the heat energy, may be applied until the tissue is substantially free of paraffin.
1. A method for of deparaffinizing a tissue, the method comprising:
providing a tissue embedded with paraffin;
melting the paraffin embedding the tissue by applying heat energy to the tissue effective to melt the paraffin; and
removing the melted paraffin out from the tissue by applying an electric field effective to move the melted paraffin out from the tissue.
2. The method of claim 1 , wherein the applying heat energy is performed by one selected from the group consisting of:
placing the tissue sample on a surface and heating the surface;
placing the tissue sample in a chamber and heating the chamber;
applying radiant heat energy;
applying infrared radiation; and
heating the paraffin to a temperature in a range from about 35° C. to about 700° C.; or a combination thereof.
3. The method of claim 1 , comprising wherein the applying the electric field includes producing a plasma in an ionization region to which the paraffin is exposed.
4. The method of claim 3 , wherein the produced plasma is produced from air molecules.
5. The method of claim 1 , wherein the applying the electric field comprises applying a voltage potential between a first electrode and a second electrode.
6. The method of claim 5 , wherein the applying the electric field includes producing a corona discharge in a region surrounding the first electrode, and further comprising exposing the paraffin to the corona discharge by positioning the first electrode.
7. The method of claim 5 , wherein the applying the electric field includes moving the first electrode relative to the tissue in a direction.
8. The method of claim 1 , wherein the applying the electric field includes moving the electric field relative to the tissue in a direction.
9. The method of claim 1 , further comprising removing water from the tissue.
10. The method of claim 1 , further comprising staining the tissue after removing the melted paraffin.
11. The method of claim 10 , wherein the staining the tissue comprises staining with an immunohistochemical staining agent.
12. The method of claim 1 , further comprising isolating nucleic acids from the tissue after removing the melted paraffin.
13. The method of claim 1 , wherein the providing the tissue comprises
placing the tissue on a solid substrate;
placing the tissue on a slide;
placing the tissue on a plate;
placing the tissue on beads;
placing the tissue on a porous medium;
placing the tissue on a filter;
placing the tissue in a liquid container; or
placing the tissue on a substrate surface having a higher surface energy than the melted paraffin.
14. A method of preparing a tissue, the method comprising:
providing a tissue embedded with paraffin; and
rendering the tissue substantially free of the paraffin by removing the paraffin from the tissue with no liquid being added to the tissue, wherein the removing the paraffin from the tissue with no liquid being added to the tissue comprises:
melting the paraffin embedded in the tissue by applying heat energy to the tissue effective to melt the paraffin; and
preparing the tissue by removing the melted paraffin by applying an electric field effective to remove the paraffin out from the tissue; and continuing to apply the electric field until the tissue is substantially free of paraffin.
15. A tissue preparation device comprising:
a heating device configured to apply thermal energy to a paraffin-embedded tissue effective to melt the paraffin embedded in the tissue; and
an electric field-generating device configured to apply an electrical field to the paraffin-embedded tissue effective to move the paraffin out from the tissue.
16. The tissue preparation device of claim 15 , wherein the electric field-generating device comprises a first electrode and a second electrode.
17. The tissue preparation device of claim 16 , wherein the electric field-generating device is configured to produce a corona discharge in a region surrounding the first electrode.
18. The tissue preparation device of claim 16 , wherein the first electrode has a structure selected from the group consisting of:
a curved structure configured to generate an electric field having a strength at a region surrounding the first electrode;
an edge configured to generate an electric field having a strength at a region surrounding the first electrode; and
a tip configured to generate electric field having a strength at a region surrounding the first electrode.
19. The tissue preparation device of claim 15 , wherein the heating device comprises a resistive heating element.
20. The tissue preparation device of claim 15 , wherein the heating device comprises a radiant heating source.