IP Library › Granted Patent US 12,338,446
Granted Patent B2
US 12,338,446 · App. 17/855,164 · Granted Jun 24, 2025

Plants having increased tolerance to herbicides

Inventors: Raphael Aponte (Mannheim, DE); Stefan Tresch (Ludwigshafen, DE); Dario Massa (Limburgerhof, DE); Tobias Seiser (Mannheim, DE); Thomas Mietzner (Annweiler, DE)
Assignee: BASF AGRO B.V.
C12N15/8274C12N9/001C12Y103/03004
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Quick Facts
Patent No.
US 12,338,446
App. No.
17/855,164
Granted
Jun 24, 2025
Kind
B2
Abstract

The present invention refers to a method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of providing, at said site, a plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding a wild-type or a mutated protoporphyrinogen oxidase (PPO) which is resistant or tolerant to a PPO-inhibiting herbicide by applying to said site an effective amount of said herbicide. The invention further refers to plants comprising wild-type or mutated PPO enzymes, and methods of obtaining such plants.

Claims (5)

1. A method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of:

a) providing, at said site, a plant that comprises a nucleic acid encoding a protoporphyrinogen oxidase (PPO), wherein expression of the nucleic acid in the plant results in increased tolerance to 1,5-dimethyl-6-thioxo-3-(2,2,7-trifluoro-3-oxo-4-(prop-2-ynyl)-3,4-dihydro-2H-benzo[b][1,4]oxazin-6-yl)-1,3,5-triazinane-2,4-dione as compared to a wild type control plant, and wherein the encoded PPO comprises the amino acid sequence of SEQ ID NO: 184; and

b) applying to said site an effective amount of 1,5-dimethyl-6-thioxo-3-(2,2,7-trifluoro-3-oxo-4-(prop-2-ynyl)-3,4-dihydro-2H-benzo[b][1,4]oxazin-6-yl)-1,3,5-triazinane-2,4-dione.

2. The method according to claim 1 , wherein the plant comprises at least one additional heterologous nucleic acid comprising a nucleotide sequence encoding a herbicide tolerance enzyme.

3. The method according to claim 2 , wherein 1,5-dimethyl-6-thioxo-3-(2,2,7-trifluoro-3-oxo-4-(prop-2-ynyl)-3,4-dihydro-2H-benzo[b][1,4]oxazin-6-yl)-1,3,5-triazinane-2,4-dione is applied in conjunction with one or more additional herbicides.

Priority Claims (1)
EP 15172587 · Jun 17, 2015 · regional
Continuity (2)
Continuation 15736423
Related Publication 20230036177A1 · Feb 2, 2023
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