Granted Patent B2

US 12,338,469 · App. 17/606,796 · Granted Jun 24, 2025

Inventors: Nina Mussmann (Willich, DE); Susanne Wieland (Zons/Dormagen, DE); Christian Degering (Erkrath, DE); Layla Fernandez (Cologne, DE); Sabine Griemert (Monheim am Rhein, DE); Barbara Peiffer (Rommerskirchen, DE); Michael Puls (Monheim am Rhein, DE); Inken Prueser (Duesseldorf, DE)

Assignee: HENKEL AG & CO. KGAA

C12N9/54C11D3/38618C11D2111/12C11D2111/14C12Y304/21062

Abstract

Claims (28)

1. A protease comprising:

an amino acid sequence having at least 70% sequence identity with the amino acid sequence given in SEQ ID NO:1;

wherein, the amino acid sequence contains:

(i) amino acid substitutions at positions 9, 144, 252, and 271; and

(ii) at least one amino acid substitution at positions 53, 120, 133, 149, 189, 211, and 274; and/or

(iii) at least one amino acid substitution, selected from the group consisting of 130Q, 130T, 130V, 130R, 131H, 159S, 162E, 162S, 172E, 189T, and combinations thereof.

2. The protease according to claim 1 , wherein:

a) the amino acid substitution at position 131 is 131H;

b) the amino acid substitution at position 53 is 53T;

c) the amino acid substitution at position 120 is 120N;

d) the amino acid substitution at position 159 is 159S;

e) the amino acid substitution at position 162 is 162E;

f) the amino acid substitution at position 172 is 172E;

g) the amino acid substitution at position 189 is 189T;

h) the amino acid substitution at position 162 is 162S;

j) the amino acid substitution at position 211 is 211N;

i) the amino acid substitution at position 274 is 274C; and and combinations thereof.

3. The protease according to claim 1 , further comprising:

one or more conservative amino acid substitutions, or fragmentation, deletion, insertion, substitution mutagenesis, or combinations thereof.

4. A nucleic acid that codes for a protease according to claim 1 .

5. A vector comprising a nucleic acid according to claim 4 .

6. A non-human host cell comprising a protease according to claim 1 .

7. A method for producing a protease, wherein the method comprises:

a) cultivating a host cell according to claim 6 ; and

b) isolating the protease from the culture medium or from the host cell.

8. An agent comprising at least one protease according to claim 1 .

9. A method for cleaning textiles or hard surfaces, wherein the method comprises:

applying the agent of claim 8 to a textile, hard surface, or combinations thereof.

Assignments (1)

Priority Claims (1)

Continuity (1)

References Cited (21)