Reagent cartridges for in-vitro devices
A method and device for displacing fluid from a reagent cartridge ( 310 ) into a microfluidic device ( 320 ) and for loading the fluid into the reagent cartridge ( 310 ). The reagent cartridge ( 310 ) may include a cartridge body and a pipette array with pipette tips ( 315 ) to engage inlets of a microfluidics or other cartridge, wherein the pipette tips ( 315 ) correspond in position to the plurality of inlets ( 325 ) of the microfluidic device ( 320 ). Fluid may be loaded into or displaced from the microfluidic device ( 320 ) by a system of plungers ( 615 ). The reagent cartridge may alternatively include blisters ( 925 ) having fluid reservoirs and dispensing tips ( 930 ), each dispensing tip ( 930 ) including a pathway ( 927 ) that is fluidly coupled to a blister ( 925 ). The fluid may be displaced from or loaded into the blister ( 925 ) via the dispensing tip ( 930 ). A deformable seal ( 910 ) may be overlaid on the blisters ( 925 ) to seal the volumes of fluid within the blisters ( 925 ), and may be deformed to displace the fluid.
1. A method of transferring reagents to a microfluidic cartridge comprising: positioning a reagent cartridge over a microfluidic device, wherein:
the reagent cartridge comprises: a substrate comprising:
one or more blisters, wherein each blister comprises a fluid reservoir configured to hold a volume of fluid; and
one or more dispensing tips, each dispensing tip comprising a pathway that is fluidly coupled to a blister, wherein a fluid is configured to be displaced from or loaded into the blister via the dispensing tip; and
one or more deformable seals fixed to the substrate and overlaid on the one or more blisters, such that the one or more deformable seals are configured to seal the volumes of fluid within the one or more blisters; and
the microfluidic device comprises a first inlet opening that is fluidly coupled to a first reservoir of the microfluidic device;
wherein positioning the reagent cartridge comprises aligning a first dispensing tip of the one or more dispensing tips with the first inlet opening such that the first inlet opening is configured to receive a first fluid from a first blister fluidly coupled to the first dispensing tip; and
displacing one or more of the deformable seals, such that the one or more deformable seals are configured to cause a first volume of the first fluid to be displaced from the first blister into the first reservoir via the first inlet opening.
2. The method of claim 1 , wherein the blisters comprise hollow cavities in a surface of the substrate.
3. The method of claim 1 , further comprising removing a base from the reagent cartridge, wherein the base is configured to engage one or more openings of the one or more dispensing tips and form a seal.
4. The method of claim 3 , wherein the base comprises one or more recessed portions configured to house at least a portion of the dispensing tips.
5. The method of claim 4 , wherein the base further comprises one or more sealing pads disposed within the one or more recessed portions, wherein each sealing pad is configured to engage and seal an opening of a respective dispensing tip.
6. The method of claim 1 , wherein the one or more deformable seals comprises a thermoplastic film.
7. The method of claim 1 , wherein the one or more deformable seals comprises a thermoplastic elastomer film.
8. The method of claim 1 , wherein the one or more deformable seals comprises a coating configured to reduce gas permeability.
9. The method of claim 1 , wherein the one or more deformable seals are fixed to the substrate by laser welding or thermal lamination.
10. The method of claim 1 , wherein the one or more deformable seals are fixed to the substrate using an adhesive.
11. The method of claim 1 , wherein the one or more deformable seals are fixed to the substrate using a pressure-sensitive adhesive.
12. The method of claim 1 , wherein the substrate comprises a plurality of blisters organized in a blister array, and wherein the one or more deformable seals comprise a single deformable film that overlays the blister array.
13. The method of claim 12 , wherein the plurality of blisters comprises a first blister having a first fluid reservoir and a second blister having a second fluid reservoir.
14. The method of claim 1 , further comprising:
inserting a dispensing needle into an opening of a first dispensing tip coupled to a first blister;
dispensing a first volume of fluid into a first blister via the dispensing needle.
15. The method of claim 1 , wherein displacing the one or more deformable seals comprises applying a first plunger end against the one or more deformable seals.
16. The method of claim 1 , wherein the microfluidic device is a cartridge.
17. The method of claim 15 , wherein the first plunger end is configured to conform to a shape and size of the fluid reservoir of the first blister.
18. The method of claim 15 , wherein the substrate comprises a plurality of blisters,
wherein the plurality of blisters comprises a first blister having a first fluid reservoir and a second blister having a second fluid reservoir, and
wherein the first plunger end is configured to conform to a shape and size of the fluid reservoir of the first blister and a second plunger end is configured to conform to a shape and size of a fluid reservoir of the second blister.
19. A method of transferring reagents to a microfluidic cartridge comprising: displacing a first deformable seal of a reagent cartridge, wherein the reagent cartridge comprises:
a substrate comprising:
one or more blisters, wherein each blister comprises a fluid reservoir configured to hold a volume of fluid; and
one or more dispensing tips, each dispensing tip comprising a pathway that is fluidly coupled to a blister,
wherein a fluid is configured to be displaced from or loaded into the blister via the dispensing tip; and
one or more deformable seals fixed to the substrate and overlaid on the one or more blisters, such that the one or more deformable seals are configured to seal the volumes of fluid within the one or more blisters;
wherein when displacing the first deformable seal, the deformable seal is configured to cause a first volume of a first fluid to be displaced from a first blister of the one or more blisters via a first dispensing tip.
20. The method of claim 19 , further comprising positioning the reagent cartridge over a microfluidic device, and
wherein the first volume of the first fluid is configured to be displaced into microfluidic device via the first inlet opening.
21. The method of claim 19 , wherein the first volume of the first fluid is configured to be displaced into a first reservoir via the first inlet opening.