IP Library › Granted Patent US 12,344,864
Granted Patent B2
US 12,344,864 · App. 17/769,655 · Granted Jul 1, 2025

Artificially synthesized mRNA and use of same

Inventors: Shinichi Hoshino (Nagoya, JP); Nao Hosoda (Nagoya, JP)
Assignee: PUBLIC UNIVERSITY CORPORATION NAGOYA CITY UNIVERSITY
C12N9/0008C07K14/47C07K14/4717C12N9/0006C12Y101/01027C12Y102/01012
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Quick Facts
Patent No.
US 12,344,864
App. No.
17/769,655
Granted
Jul 1, 2025
Kind
B2
Abstract

Provided is a technique for improving the translation efficiency of mRNA. The mRNA includes: a 5′ untranslated region of an mRNA encoding a protein; and a 3′ untranslated region having 40% or more and 80% or less complementarity to 5′ untranslated region.

Claims (16)

1. An artificially synthesized mRNA comprising a 5′ untranslated region (5′ UTR) of an mRNA encoding a protein, wherein the 5′ UTR of comprises SEQ ID NO: 27 and encoding glyceraldehyde-3-phosphate dehydrogenase; and

a 3′ untranslated region (3′ UTR) having 50% or more and 80% or less complementarity to the 5′ UTR.

2. The artificially synthesized mRNA according to claim 1 , wherein

the 3′ untranslated region has 50% or more and 75% or less complementarity to the 5′ untranslated region.

3. A method comprising the step of introducing the artificially synthesized mRNA according to claim 1 into an isolated cell, wherein said mRNA further comprises a coding region containing a gene of interest located between said 5′ untranslated region and said 3′ untranslated region and said gene of interest can be expressed in said isolated cell.

4. An isolated cell comprising the artificially synthesized mRNA according to claim 1 introduced thereinto, wherein said mRNA further comprises a coding region containing a gene of interest located between said 5′ untranslated region and said 3′ untranslated region and said gene of interest can be expressed in said isolated cell.

5. A method for manufacturing an artificially synthesized mRNA, comprising the step of:

preparing an artificially synthesized mRNA which comprises: a 5′ untranslated region (5′ UTR) of an mRNA encoding a protein; and a 3′ untranslated region (3′ UTR) having 50% or more and 80% or less complementarity to the 5′ UTR, wherein the 5′ UTR comprises SEQ ID NO: 27 and encoding glyceraldehyde-3-phosphate dehydrogenase.

6. A method for manufacturing an artificially synthesized mRNA, comprising the step of:

preparing an mRNA which comprises a 5′ untranslated region (5′ UTR) of an mRNA, wherein the 5′ UTR comprises SEQ ID NO:27 and encoding glyceraldehyde-3-phosphate dehydrogenase; and a 3′ untranslated region (3′ UTR) of the mRNA and encoding glyceraldehyde-3-phosphate dehydrogenase.

7. A method comprising the step of:

introducing an artificially synthesized mRNA comprising a 5′ untranslated region (5′ UTR) of an mRNA encoding glyceraldehyde-3-phosphate dehydrogenase, wherein the 5′ UTR comprises SEQ ID NO:27; and a 3′ untranslated region (3′ UTR) of the mRNA encoding glyceraldehyde-3-phosphate dehydrogenase into an isolated cell, wherein said mRNA further comprises a coding region containing a gene of interest located between said 5′ UTR and said 3′ UTR and said gene of interest can be expressed in said isolated cell.

8. An isolated cell comprising an artificially synthesized mRNA comprising a 5′ untranslated region (5′ UTR) of an mRNA encoding glyceraldehyde-3-phosphate dehydrogenase, wherein the 5′ UTR comprises SEQ ID NO:27 and a 3′ untranslated region (3′ UTR) of the mRNA encoding glyceraldehyde-3-phosphate dehydrogenase introduced thereinto, wherein said mRNA further comprises a coding region containing a gene of interest located between said 5′ UTR and said 3′ UTR and said gene of interest can be expressed in said isolated cell.

9. The artificially synthesized mRNA according to claim 1 , wherein said artificially synthesized mRNA further comprises a coding region located between said 5′ UTR and said 3′ UTR.

10. The method according to claim 6 , wherein said artificially synthesized mRNA further comprises a coding region located between said 5′ UTR and said 3′ UTR.

11. The method according to claim 10 , wherein said coding region contains a gene of interest located between said 5′ UTR and said 3′ UTR.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 18, 2022
From: HOSHINO, SHINICHI; HOSODA, NAO
To: PUBLIC UNIVERSITY CORPORATION NAGOYA CITY UNIVERSITY
Reel/Frame 059628/0178 →
Priority Claims (1)
JP 2019-189929 · Oct 17, 2019 · national
Continuity (1)
Related Publication 20240026311A1 · Jan 25, 2024
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