T-channel microfluidic devices and 3D printing methods for producing T-channel microfluidic devices
This disclosure, and the exemplary embodiments provided herein, include microfluidic devices and methods of producing microfluidic devices including 3D-printable structures which are scalable, robust, parallel, fast, and efficient for generating vast networks of electrodes in-situ. For example, benthic microbacterial fuel cells including networks of electrodes to harvest electrons ejected from bacteria positioned in a complex multilevel structure containing those bacteria suspended in aqueous solution or feeding medium. In addition to biofuel cells, the use of 3D-printed T channels as disclosed extends to other applications where similar networks of conducting channels can be rapidly and efficiently generated in existing structures.
1. A microfluidic device structure comprising:
a microfluidic device body;
a plurality of channels within the microfluidic device body, wherein each channel has a T-shaped cross-section, each channel comprising a top wide section and a bottom narrow section, the top wide section associated with the top of the T-shaped cross section and the bottom narrow section associated with the bottom of the T-shaped cross section, wherein the top wide section has less fluidic resistance relative to a fluidic resistance of the bottom narrow section;
wherein the top wide section is configured for passing aqueous fluid flow and the bottom narrow section is configured to have a hydrophobic fluid remain, and the bottom narrow section is configured to capture electrons from the passing aqueous fluid flow to provide a power output;
a fluidic inlet operatively associated with the microfluidic device body and the plurality of channels; and
a fluidic outlet operatively associated with the microfluidic device body and the plurality of channels.
2. The microfluidic device structure according to claim 1 , wherein the microfluidic device body is a monolithic 3D printed device.
3. The microfluidic device structure according to claim 1 , wherein the plurality of channels is filled based on surface tension and self-assembly, and the bottom narrow section of each channel is filled with first material, and the top wide section of each channel is filled with a second material distinct from the first material.
4. The microfluidic device structure according to claim 3 , wherein the microfluidic device body material is a hydrophobic material, the bottom narrow section material is a hydrophobic material, and the top wide section material is a hydrophilic material.
5. The microfluidic device structure according to claim 1 , wherein a second microfluidic structure configuration is produced by flowing the hydrophobic fluid through the plurality of channels until they are filled, then flowing a hydrophilic fluid through the plurality of filled channels until the hydrophobic fluid within top wide sections is replaced with the hydrophilic fluid and bottom narrow sections remain filled with the hydrophobic fluid.
6. The microfluidic device structure according to claim 5 , wherein the hydrophilic fluid is removed or evaporated to produce a third and final microfluidic structure configuration, wherein the bottom narrow sections are filled with the hydrophobic fluid while the top wide sections are empty.
7. The microfluidic device structure according to claim 5 , wherein the bottom narrow sections in the final configuration are filled with hydrophobic conducting fluid or conducting gel, and the top wide sections are filled with one of a hydrophilic fluid and a gas, or are empty.
8. The microfluidic device structure according to claim 5 , wherein the final configuration of the microfluidic device structure is used to transfer electrical charge from a working fluid passed through the top wide section to the conducting fluid or gel in the bottom narrow section which act as a capture electrode, and the conducting fluid or gel serves to transfer the electrical charge to an external device.
9. The microfluidic device structure according to claim 8 , wherein the working fluid is a feed solution including salt water or food medium containing microbacteria, and the conducting fluid captures electrical charge emitted by the microbacteria and conducts the electrical charge to the external device.
10. The microfluidic device structure according to claim 1 , wherein each top wide section is operatively connected to multiple narrow sections sharing a common channel associated with the top wide section.
11. The microfluidic device structure according to claim 1 , wherein the hydrophobic fluid is a gel configured to solidify.
12. A microbial fuel cell device comprising:
a monolithic 3D printed microfluidic device body;
a plurality of channels within the microfluidic device body, wherein each channel has a T-shaped cross-section, each channel including a top wide section and a bottom narrow section, the top wide section associated with the top of the T-shaped cross section and the bottom narrow section associated with the bottom of the T-shaped cross section, wherein the top wide section has less fluidic resistance relative to a fluidic resistance of the bottom narrow section, wherein the top wide section is configured for passing aqueous fluid flow, and wherein the bottom narrow section is filled with a conducting fluid or gel and the bottom narrow section is configured to capture electrons from the passing aqueous fluid flow to provide a power output;
a fluidic inlet operatively associated with the microfluidic device body and the plurality of channels; and
a fluidic outlet operatively associated with the microfluidic device body and the plurality of channels.
13. The microbial fuel cell device according to claim 12 , wherein a second microfluidic structure configuration is produced by flowing a hydrophobic fluid through the plurality of channels until they are filled, then flowing a hydrophilic fluid through the plurality of filled channels until the hydrophobic fluid within top wide sections is replaced with the hydrophilic fluid and bottom narrow sections remain filled with the hydrophobic fluid, and wherein the hydrophilic fluid is removed or evaporated to produce a third and final microfluidic structure configuration, wherein the bottom narrow sections are filled with the hydrophobic fluid while the top wide sections are empty.
14. The microbial fuel cell device according to claim 12 , wherein the microbial fuel cell device is produced by flowing a hydrophobic fluid through the plurality of channels until they are filled, then flowing a hydrophilic fluid through the plurality of filled channels until the hydrophobic fluid within top wide sections is replaced with the hydrophilic fluid and bottom narrow sections remain filled with the hydrophobic fluid, and wherein the hydrophilic fluid is removed or evaporated to produce a final microfluidic structure configuration, wherein the bottom narrow sections are filled with the hydrophobic fluid while the top wide sections are empty.
15. The microbial fuel cell device according to claim 12 , wherein the monolithic 3D printed microfluidic device is operatively associated with an array of microbial fuel cell devices which are configured as a 3D power cube.
16. The microbial fuel cell device according to claim 15 , wherein a plurality of arrayed microbial fuel cell devices configured as 3D power cube are arrayed into a superarray of 3D power cubes.
17. The microbial fuel cell device according to claim 12 , wherein the bottom narrow section is configured as an electrical conducting channel.
18. The microbial fuel cell device according to claim 17 , further configured with multiple electrical conducting channels and wherein the multiple electrical conducting channels are associated with the top wide section.