Granted Patent B2

US 12,361,668 · App. 17/811,769 · Granted Jul 15, 2025

Inventors: Jun Ki Kim (Seoul, KR); Woo June Choi (Seoul, KR)

Assignee: CHUNG ANG UNIVERSITY INDUSTRY ACADEMIC COOPERATION FOUNDATION

G06V10/25G06T7/0014G06T7/11G06V10/761G06F2218/10G06T2207/10056G06T2207/30024

Abstract

Claims (48)

1. A method for measuring motility of ciliated cells in a respiratory tract, the method comprising:

acquiring, through a microscope, first image data including a plurality of first frames of respiratory tract organoids;

identifying positions of ciliated cells by performing motion-contrast imaging on the first image data;

receiving a selection of a region of interest (ROI) related to the position of the ciliated cells;

measuring a ciliary beat frequency (CBF) related to motility of cilia included in the selected ROI, using cross-correlation between the plurality of frames;

expressing the cilia included in the ROI in a preset display method, based on a range of the measured CBF,

wherein the measuring the CBF comprises digitizing image similarity between a reference frame among the plurality of frames and remaining frames into a correlation coefficient;

acquiring, through the microscope, second image data, in which a focus is moved from the first image data, for a plurality of second frames of respiratory tract organoids;

identifying positions of ciliated cells by performing motion-contrast imaging on the second image data;

measuring the CBF related to motility of cilia included in the same ROI as the selected ROI, using cross-correlation between the plurality of second frames; and

expressing the same cilia included in the same ROI in the preset display method, based on the range of the measured CBF.

2. The method according to claim 1 , wherein the identifying the positions of ciliated cells comprises:

extracting a dynamic signal component according to motions of the ciliary cells among data components included in the first image data; and

identifying the positions of the ciliated cells based on the extracted dynamic signal component.

3. The method according to claim 1 , wherein the measuring the CBF comprises:

measuring the CBF of each ROI of a plurality of regions of interest, and

wherein the expressing the cilia comprises:

displaying a respective ROI of the plurality of regions of interest in a particular color corresponding to a respective range of a respective measured CBF.

4. The method according to claim 1 , wherein the correlation coefficient has a waveform vibrating at a predetermined period.

5. The method according to claim 4 , wherein the measuring the CBF comprises:

calculating a number of peaks of the waveform in the CBF.

6. A non-transitory computer-readable recording medium combined with a computer, which is hardware, and storing a computer program for executing the method according to claim 1 .

7. An apparatus for measuring motility of ciliated cells in a respiratory tract, the apparatus comprising:

a communication unit;

a memory; and

a processor configured to:

acquire, through a microscope, first image data including a plurality of first frames of respiratory tract organoids;

identify positions of ciliated cells by performing motion-contrast imaging on the first image data;

receive a selection of a region of interest (ROI) related to the position of the ciliated cells;

measure a ciliary beat frequency (CBF) related to motility of cilia included in the selected ROI, using cross-correlation between the plurality of frames;

express the cilia included in the ROI in a preset display method, based on a range of the measured CBF,

wherein the processor measures the CBF by digitizing image similarity between a reference frame among the plurality of frames and remaining frames into a correlation coefficient;

acquire, through the microscope, second image data, in which a focus is moved from the first image data, for a plurality of second frames of respiratory tract organoids;

identify positions of ciliated cells by performing motion-contrast imaging on the second image data;

measure the CBF related to motility of cilia included in the same ROI as the selected ROI, using cross-correlation between the plurality of second frames; and

express the same cilia included in the same ROI in the preset display method, based on the range of the measured CBF.

8. The apparatus according to claim 7 , wherein the processor is further configured to:

extract a dynamic signal component according to motions of the ciliary cells among data components included in the first image data; and

identify the positions the ciliated cells based on the extracted dynamic signal component.

9. The apparatus according to claim 7 , wherein the processor is further configured to:

measure the CBF of each ROI of a plurality of regions of interest; and

display a respective ROI of the plurality of regions of interest in a particular color corresponding to a respective range of a respective measured CBF.

10. The apparatus according to claim 7 , wherein the correlation coefficient has a waveform vibrating at a predetermined period.

11. The apparatus according to claim 10 , wherein the processor is further configured to:

calculate the number of peaks of the CBF.

12. The method according to claim 1 , further comprising:

repeating, for each organoid of the respiratory tract organoids, the acquiring the first image data, the identifying the positions of ciliated cells, the receiving the selection of the ROI, the measuring the CBF; and

displaying a color map for each organoid of the respiratory tract organoids, which expresses each cilia in a different color.

Assignments (1)

Priority Claims (1)

Continuity (1)

References Cited (12)

Cited By (1)

US 12,406,764