Granted Patent
B2
US 12,365,909 · App. 17/669,783 · Granted Jul 22, 2025
Inbred transgenic canola line NS-B50027-4 and seeds thereof
Inventors:
Malcolm Devine (Calgary, CA); Antonio Leonforte (Horsham, AU); Nelson Gororo (Horsham, AU); Greg Buzza (Horsham, AU); Shunxue Tang (West Sacramento, CA); Wenxiang Gao (West Sacramento, CA)
Assignee:
NUSEED NUTRITIONAL AUSTRALIA PTY LTD
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Abstract
The present embodiments relate to inbred transgenic canola line NS-B50027-4; seeds and oils obtained from NS-B50027-4; and progeny derived from NS-B50027-4. In particular, NS-B50027-4 is a true-breeding canola line capable of producing at least 5% DHA in its seed oil.
Claims (5)
1. A segregant of inbred canola line NS-B50027-4, having been deposited under ATCC Accession Number PTA-123186, wherein said NS-B50027-4 contains within its genome:
a first transgenic locus located on chromosome A02 comprising one copy of each of a Micromonas pusilla Δ6-desaturase, a Pyramimonas cordata Δ5-elongase, a Pavlova salina Δ5-desaturase, and a Pichia pastoris Δ15/ω3-desaturase gene, and
a second transgenic locus located on chromosome A05 comprising two copies of each of a M. pusilla Δ6-desaturase, a P. cordata Δ5-elongase, a P. salina Δ5-desaturase, a P. pastoris Δ15/ω3-desaturase, a Pavlova salina Δ4-desaturase, a Lachancea kluyveri Δ12-desaturase, and a P. cordata Δ6-elongase gene; and
wherein said segregant is a Brassica plant that comprises within its genome only said first transgenic locus.
2. A part of the segregant Brassica plant of claim 1 , wherein said part is at least one of seed, leaf, pollen, embryo, root, root tip, pod, flower, ovules, stalk, cell, protoplast, cotyledon, half-cotyledon, hypocotyl, radicle, cell culture, tissue culture, or gamete, and wherein said part comprises within its genome only said first transgenic locus.
Continuity (4)
Provisional Application
62351250
· Jun 16, 2016
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