Granted Patent B2

US 12,366,512 · App. 17/759,738 · Granted Jul 22, 2025

Inventors: Seemantini Nadkarni (Boston, MA); Zeinab Hajjarian-Kashany (Boston, MA)

Assignee: The General Hospital Corporation

G01N15/0211G01N15/01G01N2015/0222

Abstract

Claims (60)

1. A method for non-destructively estimating an average size of scattering particles in a sample, comprising:

transmitting, using a coherent light source, polarized light to the sample;

obtaining, using a detector, polarized light reflected from the sample as a function of time, the polarized light comprising a parallel polarized component and a perpendicular polarized component;

determining, using a processor, speckle decorrelation rates for the parallel polarized component and the perpendicular polarized component,

wherein determining the speckle decorrelation rates for the parallel polarized component and the perpendicular polarized component comprises determining relaxation curves for the parallel polarized component and the perpendicular polarized component based on the polarized light reflected from the sample as a function of time; and

estimating, using the processor, the average size of scattering particles in the sample based on the relaxation curves for the parallel polarized component and the perpendicular polarized component.

2. The method of claim 1 , wherein the detector comprises a plurality of pixels corresponding to a plurality of spatially-distributed locations within the sample, and

wherein obtaining polarized light reflected from the sample comprises:

obtaining polarized light reflected from the sample across the plurality of pixels,

wherein determining speckle decorrelation rates for the parallel polarized component and the perpendicular polarized component comprises:

determining speckle decorrelation rates for the parallel polarized component and the perpendicular polarized component for each of the plurality of pixels, and

wherein estimating the average size of scattering particles in the sample comprises:

estimating the average size of scattering particles for each of the plurality of pixels corresponding to each of the plurality of spatially-distributed locations within the sample.

3. The method of claim 1 , wherein estimating the average size of scattering particles in the sample based on the speckle decorrelation rates for the parallel polarized component and the perpendicular polarized component comprises:

estimating the average size of scattering particles in the sample based on determining a ratio of the relaxation curves for the parallel polarized component and the perpendicular polarized component of the polarized light reflected from the sample as a function of time.

4. The method of claim 1 , wherein determining speckle decorrelation rates for the parallel polarized component and the perpendicular polarized component comprises:

determining speckle decorrelation rates for the parallel polarized component and the perpendicular polarized component based on a table of values.

5. The method of claim 4 , wherein the table of values is generated based on a simulation of speckle density autocorrelation curves for a plurality of particle sizes.

6. The method of claim 5 , wherein the simulation comprises a Polarization Sensitive Correlation Transfer Monte Carlo Ray Tracing simulation.

7. The method of claim 1 , wherein transmitting linearly polarized light to the sample comprises:

transmitting linearly polarized light comprising a plurality of wavelengths to the sample; and

wherein estimating the average size of scattering particles in the sample comprises:

estimating the average size of scattering particles in the sample based on transmitting linearly polarized light comprising a plurality of wavelengths to the sample.

8. The method of claim 1 , wherein transmitting linearly polarized light to the sample comprises:

alternately transmitting parallel polarized light and perpendicular polarized light to the sample.

9. The method of claim 8 , wherein alternately transmitting parallel polarized light and perpendicular polarized light to the sample comprises:

modulating between parallel polarized light and perpendicular polarized light using a polarization modulator in combination with an optical isolator or polarization isolator based on switching mirrors to select one of the parallel polarized light and the perpendicular polarized light.

10. The method of claim 1 , wherein the polarized light comprises linearly polarized light.

11. An apparatus for non-destructively estimating an average size of scattering particles in a sample, comprising:

a coherent light source;

a detector; and

a processor in communication with the coherent light source and the detector, the processor being configured to:

control the coherent light source to transmit polarized light to the sample,

control the detector to obtain polarized light reflected from the sample,

the polarized light comprising a parallel polarized component and a perpendicular polarized component,

determine speckle decorrelation rates for the parallel polarized component and the perpendicular polarized component,

wherein determining the speckle decorrelation rates for the parallel polarized component and the perpendicular polarized component comprises determining relaxation curves for the parallel polarized component and the perpendicular polarized component based on the polarized light reflected from the sample as a function of time, and

estimate the average size of scattering particles in the sample based on the relaxation curves for the parallel polarized component and the perpendicular polarized component.

12. The apparatus of claim 11 , wherein the detector comprises a plurality of pixels corresponding to a plurality of spatially-distributed locations within the sample, and

wherein the processor, when obtaining polarized light reflected from the sample, is further configured to:

obtain polarized light reflected from the sample across the plurality of pixels,

wherein the processor, when determining speckle decorrelation rates for the parallel polarized component and the perpendicular polarized component, is further configured to:

determine speckle decorrelation rates for the parallel polarized component and the perpendicular polarized component for each of the plurality of pixels, and

wherein the processor, when estimating the average size of scattering particles in the sample, is further configured to:

estimate the average size of scattering particles for each of the plurality of pixels corresponding to each of the plurality of spatially-distributed locations within the sample.

13. The apparatus of claim 11 , wherein the processor, when estimating the average size of scattering particles in the sample based on the speckle decorrelation rates for the parallel polarized component and the perpendicular polarized component, is further configured to:

estimate the average size of scattering particles in the sample based on determining a ratio of the relaxation curves for the parallel polarized component and the perpendicular polarized component of the polarized light reflected from the sample as a function of time.

14. The apparatus of claim 11 , wherein the processor, when determining speckle decorrelation rates for the parallel polarized component and the perpendicular polarized component, is further configured to:

determine speckle decorrelation rates for the parallel polarized component and the perpendicular polarized component based on a table of values.

15. The apparatus of claim 14 , wherein the table of values is generated based on a simulation of speckle density autocorrelation curves for a plurality of particle sizes.

16. The apparatus of claim 15 , wherein the simulation comprises a Polarization Sensitive Correlation Transfer Monte Carlo Ray Tracing simulation.

17. The apparatus of claim 11 , wherein the processor, when transmitting polarized light to the sample, is further configured to:

transmit polarized light comprising a plurality of wavelengths to the sample; and

wherein the processor, when estimating the average size of scattering particles in the sample, is further configured to:

estimate the average size of scattering particles in the sample based on transmitting polarized light comprising a plurality of wavelengths to the sample.

18. The apparatus of claim 11 , wherein the processor, when transmitting polarized light to the sample, is further configured to:

alternately transmit parallel polarized light and perpendicular polarized light to the sample.

19. The apparatus of claim 18 , wherein the processor, when alternately transmitting parallel polarized light and perpendicular polarized light to the sample, is further configured to:

modulate between parallel polarized light and perpendicular polarized light using a polarization modulator in combination with an optical isolator or polarization isolator based on switching mirrors to select one of the parallel polarized light and the perpendicular polarized light.

20. The apparatus of claim 11 , wherein the polarized light comprises linearly polarized light.

Assignments (1)

Continuity (2)

References Cited (39)