Recombinant oncolytic viruses for treatment of metastatic cancers
Disclosed are recombinant oncolytic viruses that express one or more reovirus fusion-associated small transmembrane FAST) proteins and uses thereof. The oncolytic activity of the recombinant oncolytic viruses expressing FAST proteins can be used to treat primary and metastatic cancers, especially from breast and colon cancers.
1. A method for treating breast, lung or colon cancer comprising administering to a subject in need thereof a composition consisting of (a) a recombinant oncolytic virus that expresses reovirus fusion-associated small transmembrane (FAST) proteins, wherein the FAST protein is a combination of p14/p15 comprising the ectodomain and transmembrane of p14 and the endodomain of p15; the ectodomain of p14, the transmembrane domain and endodomain of p15; or the ectodomain and endodomain of p14 and the transmembrane of p15, and (b) one or more pharmaceutically acceptable carriers or excipients.
2. The method of claim 1 , wherein the breast, lung or colon cancer is a metastatic breast, lung or colon cancer.
3. The method of claim 1 , wherein the FAST protein is p14 protein from reptilian reovirus; or a combination of p14 protein from reptilian reovirus and p15 protein from baboon reovirus.
4. The method of claim 3 , wherein the oncolytic virus is from herpesvirus, adenovirus, reovirus, measles virus, Newcastle disease virus, vaccina virus and vesicular stomatitis virus.
5. The method of claim 4 , wherein the oncolytic virus is vesicular stomatitis virus.