Method for producing cell cluster group and device for producing same
This method for producing a cell cluster group comprises: a step for putting, into a well, a cell suspension obtained by suspending dispersed cells in a medium, using a cell incubator which includes the well and two or more recesses formed in the bottom of the well and in which the area of an opening of each recess in plan view is at most 1 mm 2 ; a step for centrifuging the cell incubator; and a step for culturing the dispersed cells in the recesses.
1. A method for production of a cell cluster group, the method comprising:
adding into a well of a cell-cultivation container a cell suspension in which dispersed cells are suspended in a culture medium, wherein the well further comprises at least two recesses formed in a bottom face of the well, and wherein an area of an opening of each of the at least two recesses in plan view is 1 mm 2 or less;
centrifuging the cell-cultivation container; and
culturing the dispersed cells in the recesses,
wherein an inner wall surface of each of the at least two recesses is coated with a cell-non-adhesiveness-imparting agent; and
wherein each of the at least two recesses has a cylindrical shape or a conical shape.
2. The method of claim 1 , wherein a depth of each of the at least two recesses is from 100 μm to 1000 μm.
3. The method of claim 1 , wherein a tessellation ratio of openings of the at least two recesses in the bottom face of the well is from 5% to 99.9%.
4. The method of a cell cluster group according to claim 1 , wherein a number of the at least two recesses in the well is from 2 to 500.
5. The method of claim 1 , wherein a number of the dispersed cells in the cell suspension is from 10 cells/μL to 5,000 cells/μL.
6. The method of claim 1 , wherein an extracellular matrix is mixed in the culture medium.
7. The method of claim 1 , wherein an inner wall surface of each of the at least two recesses has cell-non-adhesiveness.
8. The method of claim 1 , wherein an inner wall surface of each of the at least two recesses recess has a fine protrusion structure.
9. The method of claim 1 , wherein the dispersed cells comprise dispersed gastrointestinal stem cells.
10. The method of claim 1 , wherein the cell-cultivation container is centrifuged at a centrifugal force of from 200×g to 1000×g.