Granted Patent
B2
US 12,378,547 · App. 18/493,307 · Granted Aug 5, 2025
Synthetic genome
Inventors:
Julius Fredens (Swindon, GB); Kaihang Wang (Swindon, GB); Daniel De La Torre (Swindon, GB); Louise F. H. Funke (Swindon, GB); Wesley E. Robertson (Swindon, GB); Jason W. Chin (Swindon, GB)
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Abstract
The current invention provides a synthetic prokaryotic genome comprising 5 or fewer occurrences of one or more sense codons; and/or a synthetic prokaryotic genome derived from a parent genome, wherein the synthetic prokaryotic genome comprises less than 10%, 5%, 2%, 1%, 0.5%, 0.1% of the occurrences of one or more sense codons, relative to the parent genome; and/or a synthetic prokaryotic genome comprising 100 or more, 200 or more, or 1000 or more genes with no occurrences of one or more sense codons.
Claims (6)
1. A synthetic E. coli genome, wherein 90% or more of occurrences of an amber stop codon (TAG) are replaced with TAA and 90% or more of occurrences of a serine codon are replaced with a synonymous codon.
2. A synthetic E. coli genome, wherein 95% or more of occurrences of an amber stop codon (TAG) are replaced with TAA and 95% or more of occurrences of a serine codon are replaced with a synonymous codon.
3. A synthetic E. coli genome, wherein 99% or more of occurrences of an amber stop codon (TAG) are replaced with TAA and 99% or more of occurrences of a serine codon are replaced with a synonymous codon.
4. A synthetic E. coli genome, wherein 100% of occurrences of an amber stop codon (TAG) are replaced with TAA and 100% of occurrences of a serine codon are replaced with a synonymous codon.
5. The synthetic E. coli genome according to claim 1 , wherein an E. coli cell comprising the synthetic E. coli genome is viable.
6. The synthetic E. coli genome according to claim 1 , wherein the synthetic E. coli genome is derived from a parent E. coli genome.
Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jan 29, 2024
From: FREDENS, JULIUS; WANG, KAIHANG; DE LA TORRE, DANIEL; FUNKE, LOUISE F. H.; ROBERTSON, WESLEY E.; CHIN, JASON W.
To: UNITED KINGDOM RESEARCH AND INNOVATION
Reel/Frame 066280/0296 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jan 29, 2024
From: UNITED KINGDOM RESEARCH AND INNOVATION
To: SCARAB GENOMICS, LLC; UNITED KINGDOM RESEARCH AND INNOVATION
Reel/Frame 066280/0401 →
Continuity (4)
Continuation
17610974
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