IP Library › Granted Patent US 12,378,586
Granted Patent B2
US 12,378,586 · App. 17/905,047 · Granted Aug 5, 2025

Systems, methods, and compositions for the glycosylation of cannabinoid compounds

Inventors: Timothy Travers (Los Alamos, NM); Erick LeBrun (Los Alamos, NM)
Assignee: Trait Biosciences, Inc.
C12P17/06C12N9/1051C12Y204/01035
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Quick Facts
Patent No.
US 12,378,586
App. No.
17/905,047
Granted
Aug 5, 2025
Kind
B2
Abstract

The present invention relates generally to the identification novel UDP-glucosyltransferases enzymes having specific activity towards cannabinoid compounds. The present invention further relates generally to the use of novel UGT enzymes having specific activity towards cannabinoid compounds to generate water-soluble cannabinoid glycoside compounds.

Claims (8)

1. A system for producing glycosylated cannabidiol (CBD) comprising:

a yeast cell culture, where the yeast cells express a heterologous nucleotide sequence, operably linked to a promoter, encoding a UDP-glucosyltransferases (UGT) according to the amino acid sequence SEQ ID NO. 1, or a sequence having at least 95% sequence identity with SEQ ID NO. 1826; and

wherein CBD is introduced to the culture where the UGT glycosylates said CBD generating a CBD glycoside.

2. The system of claim 1 , wherein the yeast is Picha pastoris.

3. A system for producing glycosylated tetrahydrocannabinol (THC) comprising:

a yeast cell culture, where the yeast cells express a heterologous nucleotide sequence, operably linked to a promoter, encoding a UDP-glucosyltransferases (UGT) according to the amino acid sequence SEQ ID NO. 1, or a sequence having at least 95% sequence identity with SEQ ID NO. 1826; and

wherein THC is introduced to the culture where the UGT glycosylates said THC generating a CBD glycoside.

4. The system of claim 3 , wherein the yeast is Picha pastoris.

Continuity (2)
Provisional Application 62983019 · Feb 28, 2020
Related Publication 20230212625A1 · Jul 6, 2023
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