Closed-system sorting flow cytometer adapters and methods of use thereof
Closed-system sorting flow cytometer adapters for converting a sorting flow cytometer having a sort block into a closed-system sorting flow cytometer are provided. Adapters of interest include a sort block coupler for operably connecting to the sort block, an external chamber, and a connector for gaseously coupling the sort block to the external chamber, optionally via a sort bucket that is in cellular-receiving relationship with the sort block coupler. Flow cytometers having closed-system sorting flow cytometer adapters are also disclosed. Methods, computer systems and kits for carrying out the invention are additionally provided.
1. A sorting flow cytometer, the sorting flow cytometer comprising:
an illumination source for illuminating a flow cell at an interrogation point;
a detector for receiving light from the interrogation point;
a sort block in liquid-receiving relationship with the flow cell; and
a closed-system sorting flow cytometer adapter in droplet-receiving relationship with the sort block, the adapter comprising:
a sort block coupler operably connected to the sort block;
an external chamber; and
a connector gaseously coupling the sort block to the external chamber, optionally via a sort bucket that is in cellular-receiving relationship with the sort block coupler,
wherein the adaptor is configured to generate a closed positive pressure system within the sorting flow cytometer.
2. The sorting flow cytometer according to claim 1 , further comprising the sort bucket.
3. The sorting flow cytometer according to claim 2 , wherein the sort bucket is configured to house a plurality of collection vessels for collecting droplets received from the sort block.
4. The sorting flow cytometer according to claim 1 , wherein the sort block coupler comprises a sealing gasket.
5. The sorting flow cytometer according to claim 1 , wherein the adapter comprises a collar for attaching the connector to the external chamber.
6. The sorting flow cytometer according to claim 1 , further comprising a gas inlet port for filling the closed system with a first gas.
7. The sorting flow cytometer according to claim 6 , wherein the sorting flow cytometer comprises a plurality of gas inlet ports.
8. The sorting flow cytometer according to claim 6 , further comprising a compressor gaseously connected to the gas inlet port, wherein the compressor is configured to fill the closed system with the first gas.
9. The sorting flow cytometer according to claim 8 , further comprising a gas relief valve for allowing a second gas to escape from the closed system.
10. The sorting flow cytometer according to claim 9 , further comprising a gas sensor configured to assess the concentration of the second gas within the closed system.
11. The sorting flow cytometer according to claim 10 , wherein the gas sensor is configured to assess the concentration of oxygen.
12. The sorting flow cytometer according to claim 10 or 11 , wherein the compressor is operably connected to the gas sensor and is configured to regulate the concentration of the second gas based on the assessment from the gas sensor.
13. The sorting flow cytometer according to claim 8 , wherein the compressor is configured to fill the closed system with the first gas at a pressure ranging from 5 psi to 40 psi.
14. The sorting flow cytometer according to claim 1 , wherein the adapter further comprises an internal door configured to permit the flow of gas between the sort block and external chamber when the internal door is open and prevent the flow of gas between the sort block and external chamber when the internal door is closed.
15. The sorting flow cytometer according to claim 1 , wherein the sort block comprises a deflection plate.
16. A method of converting a sorting flow cytometer having a sort block into a closed-system flow cytometer, the method comprising operably connecting a closed-system sorting flow cytometer adapter to the sorting flow cytometer, wherein the adapter comprises:
a sort block coupler operably connected to the sort block;
an external chamber; and
a connector gaseously coupling the sort block to the external chamber, optionally via a sort bucket that is in cellular-receiving relationship with the sort block coupler,
wherein the adaptor is configured to generate a closed positive pressure system within the sorting flow cytometer.
17. The method according to claim 16 , further comprising operably connecting the sort bucket to the sort block coupler.
18. A method of sorting particles from a sample, the method comprising:
(a) introducing the particles into a sorting flow cytometer comprising:
an illumination source for illuminating a flow cell at an interrogation point;
a detector for receiving light from the interrogation point;
a sort block in liquid-receiving relationship with the flow cell; and
a closed-system sorting flow cytometer adapter in droplet-receiving relationship with the sort block, the adapter comprising:
a sort block coupler operably connected to the sort block;
an external chamber; and
a connector gaseously coupling the sort block to the external chamber, optionally via a sort bucket that is in cellular-receiving relationship with the sort block coupler,
wherein the adaptor is configured to generate a closed positive pressure system within the sorting flow cytometer; and
(b) flow cytometrically sorting the particles.
19. The method according to claim 18 , wherein the sorting flow cytometer further comprises the sort bucket.