Granted Patent
B2
US 12,385,016 · App. 17/248,157 · Granted Aug 12, 2025
Degradation of sporopollenin
Assignee:
Lafayette College
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Abstract
A composition comprised of a peroxidase, a catalase, and a peroxide that, in combination, is suitable for degradation of pollen wall sporopollenin, and coatings comprising sporopollenin in a matrix that can be selectively degraded through application of a peroxidase, a catalase, and a peroxide to the sporopollenin matrix.
Claims (6)
1. A composition suitable for degrading a sporopollenin-based coating, the composition comprising: a peroxidase obtained from a plant species, a catalase obtained from an animal species, said peroxidase at a ratio of 100:1 to the catalase, and equal amounts of each of an acidic buffer and a peroxide.
2. The composition of claim 1 wherein the peroxidase is a horseradish peroxidase.
3. The composition of claim 1 wherein the catalase is a beef liver catalase.
4. The composition of claim 1 wherein the peroxide is hydrogen peroxide.
5. The composition of claim 1 wherein the peroxidase is selected from the group consisting of: horseradish peroxidase, lignin peroxidase, and white rot fungal manganese peroxidase; the catalase is selected from the group consisting of bovine liver catalase, Corynebacterium catalase, and human erythrocytes catalase; and the peroxide is hydrogen peroxide.
6. A kit for degrading a coating comprising sporopollenin comprising: the composition of claim 1 and a multichambered applicator.
Assignments (3)
CONFIRMATORY LICENSE
Recorded Dec 22, 2023
From: LAFAYETTE COLLEGE
To: NATIONAL SCIENCE FOUNDATION
Reel/Frame 066123/0683 →
CONFIRMATORY LICENSE
Recorded Dec 22, 2023
From: LAFAYETTE COLLEGE
To: NATIONAL SCIENCE FOUNDATION
Reel/Frame 066127/0370 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jan 13, 2021
From: EDLUND, ANNA F.
To: LAFAYETTE COLLEGE
Reel/Frame 054903/0313 →
Continuity (3)
Provisional Application
62486274
· Apr 17, 2017
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