Detection of target nucleic acid molecules
View Patent ↗The present invention relates to a test apparatus, system, and method of detecting target nucleic acid molecules in a biological fluid test sample through analyzing changes in electrical parameters caused by hybridization of target nucleic acids that are complementary to the at least partially single-stranded nucleic acid template strand portion of the probe. A test disc encloses at least one set of electrically separated electrodes with the probe bridging the electrodes. The test sample, potentially containing a target virus, is introduced into a cartridge well, and the test disc is added to the well under conditions permitting hybridization. Electrical parameters within the circuit are measured to detect the presence and concentration of any hybridization complexes formed. Multiple test disc structures, including a cylindrical configuration and a cone construct, are disclosed.
1. A system for detection of a target nucleic acid molecule in a specimen, comprising:
a cartridge including a cartridge top portion, an opposing cartridge bottom portion, and a concave well disposed in said cartridge top portion; wherein said concave well comprises an open-top specimen-receiving aperture defined by a well bottom and well side walls, wherein said specimen-receiving aperture accommodates a volume of a specimen biological fluid potentially containing said target nucleic acid molecule; and
a test disc insertable into said concave well; said test disc comprising a first conductor, a second conductor electrically separated said first conductor, and probe nucleic acid molecules bridging a gap between said first conductor and said second conductor; wherein said probe nucleic acid molecules comprise at least partially single-stranded nucleic acid molecules; wherein said test disc is openable to introduce said specimen biological fluid to said probe nucleic acid molecules; and wherein, if said specimen biological fluid contains said target nucleic acid molecule hybridization occurs.
2. The system for detection of a target nucleic acid molecule in a specimen, as recited in claim 1 , wherein said test disc further comprises a truncated cone construction comprising a cone truncated top portion and a cone base; wherein said first conductor is disposed at said cone truncated top portion; wherein said second conductor is disposed at said cone base; and wherein said truncated cone construction further comprises an intermediary portion disposed between, and electrically separating, said first conductor and said second conductor.
3. The system for detection of a target nucleic acid molecule in a specimen, as recited in claim 1 , wherein said test disc further comprises disc side walls and a disc center area; wherein said first conductor is disposed at said disc center area; and wherein said second conductor comprises a cylindrical wall disposed inside of said disc side walls.
4. The system for detection of a target nucleic acid molecule in a specimen, as recited in claim 1 , wherein said probe nucleic acid molecules are attached laxly between said first conductor and said second conductor.
5. The system for detection of a target nucleic acid molecule in a specimen, as recited in claim 1 , wherein said test disc further comprises a reagent; wherein said test disc further comprises a reagent compartment that contains said reagent; and wherein said reagent compartment is openable to allow said reagent to mix with said specimen biological fluid.
6. The system for detection of a target nucleic acid molecule in a specimen, as recited in claim 5 , wherein said test disc comprises a two-compartment test disc comprising said reagent compartment and a compartment containing said probe nucleic acid molecules.
7. The system for detection of a target nucleic acid molecule in a specimen, as recited in claim 5 , wherein said specimen biological fluid comprises a viral agent containing said target nucleic acid; and wherein when said reagent is mixed with said specimen biological fluid, said viral agent is opened and said target nucleic acid is at least partially unwound to create single-strand nucleic acid.
8. The system for detection of a target nucleic acid molecule in a specimen, as recited in claim 1 , wherein said open-top specimen-receiving aperture accommodates insertion of said test disc.
9. The system for detection of a target nucleic acid molecule in a specimen, as recited in claim 1 , wherein said cartridge further comprises a disc-receiving aperture defined by an aperture roof, aperture side walls, an aperture back wall, an aperture floor, and an aperture side opening; wherein a portion of said disc-receiving aperture is disposed below said concave well; wherein said disc-receiving aperture accommodates insertion of said test disc; and wherein said concave well comprises a movable floor configured to move to allow said specimen biological fluid to feed downward.
10. The system for detection of a target nucleic acid molecule in a specimen, as recited in claim 1 further comprising a detection circuit for providing an indication of the presence or absence of said target nucleic acid molecule by measuring one or more electrical parameters; wherein said probe nucleic acid molecules are aligned in rows of probe nucleic acid molecules; and wherein said detection circuit provides an electrical reading from each of said rows of probe nucleic acid molecules or from groups of said rows of probe nucleic acid molecules.
11. The system for detection of a target nucleic acid molecule in a specimen, as recited in claim 1 , further comprising:
a detection circuit for providing an indication of the presence or absence of said target nucleic acid molecule by measuring one or more electrical parameters; and
a signaling device for receiving and displaying one or more results from said detection circuit.
12. The system for detection of a target nucleic acid molecule in a specimen, as recited in claim 1 , further comprising a disc holder for accommodating said test disc and multiple additional test discs; wherein said disc holder comprises a power source configured to supply current to said test disc and said multiple additional test discs when said test disc is installed within said disc holder with the current functional to maintain said probe nucleic acid molecules in a stretched configuration bridging said gap between said first conductor and said second conductor.
13. The system for detection of a target nucleic acid molecule in a specimen, as recited in claim 1 , wherein said probe nucleic acid molecule comprises a central template strand of nucleic acid; a nucleic acid sequence (X) attached to a head-facing end of said central template strand of nucleic acid; and a nucleic acid sequence (Y) attached to a tail-facing end of said central template strand of nucleic acid; wherein said central template strand of nucleic acid is complementary to at least a portion of said target nucleic acid molecule.