Microfluidic chip
The present disclosure provides a microfluidic chip, and belongs to the field of biological detection technology. The microfluidic chip is divided into a middle region and a peripheral region surrounding the middle region; the middle region includes a liquid storage region and a detection region; the microfluidic chip includes a first substrate and a second substrate opposite to each other; the first substrate includes a first base plate and a first electrode layer; the second substrate includes a second base plate and a second electrode layer; wherein a liquid storage tank and a liquid inlet are on a side of the first base plate proximal to the second substrate, the liquid inlet penetrates through a bottom of the liquid storage tank; the liquid storage tank and the liquid inlet are both in the liquid storage region.
1. A microfluidic chip, comprising:
a middle region and a peripheral region surrounding the middle region; wherein the middle region comprises a liquid storage region and a detection region; the microfluidic chip comprises a first substrate and a second substrate opposite to each other; the first substrate comprises a first base plate and a first electrode layer on a side of the first base plate proximal to the second substrate; the second substrate comprises a second base plate and a second electrode layer on a side of the second base plate proximal to the first substrate; wherein
the first substrate comprises a liquid storage tank on a side of the first base plate proximal to the second substrate and a liquid inlet on a side of the first base plate away from the second substrate, the liquid inlet penetrates through a surface of the liquid storage tank away from the second substrate; the liquid storage tank and the liquid inlet are both in the liquid storage region;
a cross section of the liquid storage tank taken along a thickness direction of the first substrate and a direction parallel to a direction in which the liquid storage region points towards the detection region has a first side proximal to the detection region; the first side has a first end point and a second end point, and the first end point is farther away from the second substrate than the second end point; an extending direction of a line connecting the first end point and the second end point is inclined with respect to an axial direction of the liquid inlet, and a distance from the first end point to an axis of the liquid inlet is smaller than a distance from the second end point to the axis of the liquid inlet; and
the liquid storage tank further comprises a second side opposite to the first side, and the other pair of sides arranged opposite to each other, which are a third side and a fourth side;
wherein a dihedral angle between the surface and the second side, the third side or the fourth side is 90 degrees or approximately 90 degrees.
2. The microfluidic chip according to claim 1 , wherein the first side is a straight side.
3. The microfluidic chip according to claim 1 , wherein an angle θ between the extending direction of the line connecting the first end point and the second end point and the axial direction of the liquid inlet is 30° or −60°.
4. The microfluidic chip according to claim 1 , wherein the liquid inlet comprises a first port and a second port, and the liquid inlet is communicated with the liquid storage tank through the first port; an orthographic projection of the first port on the second base plate at least partially overlaps an orthographic projection of the second electrode layer on the second base plate.
5. The microfluidic chip according to claim 4 , wherein an orthographic projection of an edge of the second electrode layer on the second base plate passes through a center of the orthographic projection of the first port on the second base plate; or
the orthographic projection of the edge of the second electrode layer on the second base plate is tangent to an edge of the orthographic projection of the first port on the second base plate.
6. The microfluidic chip according to claim 1 , wherein the liquid inlet comprises a first port and a second port opposite to each other, and the liquid inlet is communicated with the liquid storage tank through the first port; an orthographic projection of the second port on the second base plate covers an orthographic projection of the first port on the second base plate.
7. The microfluidic chip according to claim 5 , further comprising:
a first hydrophobic layer which is on a side of the first electrode layer proximal to the second substrate; and
a second hydrophobic layer which is on a side of the second electrode layer proximal to the first substrate.
8. The microfluidic chip according to claim 1 , further comprising a liquid inlet column; wherein
the liquid inlet column and the liquid inlet are coaxially arranged; the liquid inlet comprises a first port and a second port opposite to each other, and the liquid inlet is communicated with the liquid storage tank through the first port; the liquid inlet column comprises a third port and a fourth port opposite to each other; the third port of the liquid inlet column is connected with the second port of the liquid inlet.
9. The microfluidic chip according to claim 8 , wherein the first port and the second port of the liquid inlet have a same aperture.
10. The microfluidic chip according to claim 8 , further comprising:
a first hydrophobic layer which is on a side of the first electrode layer proximal to the second substrate; and
a second hydrophobic layer which is on a side of the second electrode layer proximal to the first substrate.
11. The microfluidic chip according to claim 1 , further comprising a liquid inlet column; wherein
the liquid inlet column and the liquid inlet are coaxially arranged; the liquid inlet comprises a first port and a second port opposite to each other, and the liquid inlet is communicated with the liquid storage tank through the first port; the liquid inlet column comprises a third port and a fourth port opposite to each other; an orthographic projection of the third port of the liquid inlet column on the second base plate is within an orthographic projection of the fourth port on the second base plate.
12. The microfluidic chip according to claim 11 , further comprising:
a first hydrophobic layer which is on a side of the first electrode layer proximal to the second substrate; and
a second hydrophobic layer which is on a side of the second electrode layer proximal to the first substrate.
13. The microfluidic chip according to claim 1 , further comprising a sealant which is between the first substrate and the second substrate and in the peripheral region and surrounds the middle region.
14. The microfluidic chip according to claim 13 , further comprising a first receiving groove which is on a side of the first base plate proximal to the second substrate, wherein the sealant is in the first receiving groove.
15. The microfluidic chip according to claim 1 , further comprising a conductive component which is between the first substrate and the second substrate and in the peripheral region, and is configured to electrically connect the first electrode layer with a connection pad on the second substrate.
16. The microfluidic chip according to claim 15 , further comprising a second receiving groove which is on a side of the first base plate proximal to the second substrate, wherein the conductive component is in the second receiving groove.
17. The microfluidic chip according to claim 1 , further comprising a support structure which is between the first substrate and the second substrate, and is configured to maintain a cell gap between the first substrate and the second substrate.
18. The microfluidic chip according to claim 1 , further comprising a gas outlet which is on the first base plate and penetrates through the first base plate in a thickness direction.
19. The microfluidic chip according to claim 1 , further comprising:
a first hydrophobic layer which is on a side of the first electrode layer proximal to the second substrate; and
a second hydrophobic layer which is on a side of the second electrode layer proximal to the first substrate.
20. The microfluidic chip according to claim 1 , wherein the dihedral angle between the surface and the second side is 90 degrees or approximately 90 degrees; and
the distance from the first end point to the axis of the liquid inlet is larger than a distance from the second side to the axis of the liquid inlet.