Method for breaking dormancy of
A method for breaking dormancy of Thesium chinense Turcz. seeds, which includes: immersing collected seeds of Thesium chinense Turcz. in water, selecting seeds that sink in the water followed by air-drying, and storing at 2-4° C. to obtain to-be-used seeds; immersing the to-be-used seeds in a gibberellin solution, taking out the seeds and mixing with river sand under −4-2° C. for a first stratification treatment; rinsing off the river sand, removing green skins and stalks of the seeds after the first stratification treatment, keeping the seeds moist and placing the seeds under 2-4° C. for a second stratification treatment to obtain ruptured seeds; and using the ruptured seeds in a three-slit stage as a germination material, and culturing the ruptured seeds in a three-slit stage in a dark environment at 17-22° C. for seed germination. The method can significantly improve the germination rate of Thesium chinense Turcz. seeds.
1. A method for breaking dormancy of Thesium chinense Turcz seeds, comprising:
immersing collected seeds of Thesium chinense Turcz in water, selecting seeds that sink in the water followed by air-drying, and storing at 2° C. to 4° C. to obtain to-be-used seeds;
immersing the to-be-used seeds in a gibberellin solution, taking out the seeds and mixing with river sand under −4° C. to −2° C. for a first stratification treatment;
rinsing off the river sand, removing green skins and stalks of the seeds after the first stratification treatment, keeping the seeds moist and placing the seeds under 2° C. to 4° C. for a second stratification treatment to obtain ruptured seeds; and
using the ruptured seeds in a three-slit stage as a germination material, and culturing the ruptured seeds in a three-slit stage in a dark environment at 17° C. to 22° C. for seed germination.
2. The method for breaking dormancy of Thesium chinense Turcz seeds as claimed in claim 1 , wherein the collected seeds are immersed in the water for 10-14 hours.
3. The method for breaking dormancy of Thesium chinense Turcz seeds as claimed in claim 2 , wherein in the step of placing the seeds under 2° C. to 4° C. for a second stratification treatment to obtain ruptured seeds, the ruptured seeds are rinsed with water every 6-8 days throughout the second stratification treatment of a total duration of 115-125 days.
4. The method for breaking dormancy of Thesium chinense Turcz seeds as claimed in claim 1 , further comprising, stirring, during water immersion, for 4-6 times.
5. The method for breaking dormancy of Thesium chinense Turcz seeds as claimed in claim 4 , wherein in the step of placing the seeds under 2° C. to 4° C. for a second stratification treatment to obtain ruptured seeds, the ruptured seeds are rinsed with water every 6-8 days throughout the second stratification treatment of a total duration of 115-125 days.
6. The method for breaking dormancy of Thesium chinense Turcz seeds as claimed in claim 1 , wherein a concentration of the gibberellin solution is 750-1000 mgL −1 .
7. The method for breaking dormancy of Thesium chinense Turcz seeds as claimed in claim 6 , wherein the gibberellin is GA 3 .
8. The method for breaking dormancy of Thesium chinense Turcz seeds as claimed in claim 7 , wherein in the step of placing the seeds under 2° C. to 4° C. for a second stratification treatment to obtain ruptured seeds, the ruptured seeds are rinsed with water every 6-8 days throughout the second stratification treatment of a total duration of 115-125 days.
9. The method for breaking dormancy of Thesium chinense Turcz seeds as claimed in claim 6 , wherein the to-be-used seeds are immersed in the gibberellin solution for 22-26 hours.
10. The method for breaking dormancy of Thesium chinense Turcz seeds as claimed in claim 9 , wherein, the to-be-used seeds are immersed in the gibberellin solution for 24 hours.
11. The method for breaking dormancy of Thesium chinense Turcz seeds as claimed in claim 10 , wherein in the step of placing the seeds under 2° C. to 4° C. for a second stratification treatment to obtain ruptured seeds, the ruptured seeds are rinsed with water every 6-8 days throughout the second stratification treatment of a total duration of 115-125 days.
12. The method for breaking dormancy of Thesium chinense Turcz seeds as claimed in claim 9 , wherein in the step of placing the seeds under 2° C. to 4° C. for a second stratification treatment to obtain ruptured seeds, the ruptured seeds are rinsed with water every 6-8 days throughout the second stratification treatment of a total duration of 115-125 days.
13. The method for breaking dormancy of Thesium chinense Turcz seeds as claimed in claim 6 , wherein in the step of placing the seeds under 2° C. to 4° C. for a second stratification treatment to obtain ruptured seeds, the ruptured seeds are rinsed with water every 6-8 days throughout the second stratification treatment of a total duration of 115-125 days.
14. The method for breaking dormancy of Thesium chinense Turcz seeds as claimed in claim 1 , wherein in the step of mixing with river sand under −4° C. to −2° C. for a first stratification treatment, a mass ratio of the seeds to the river sand is 1:1, and a duration of the first stratification treatment is 58-62 days.
15. The method for breaking dormancy of Thesium chinense Turcz seeds as claimed in claim 14 , wherein a water content of the river sand is such that the river sand forms a cluster under pressure and scatters when no force being applied.
16. The method for breaking dormancy of Thesium chinense Turcz seeds as claimed in claim 15 , wherein in the step of placing the seeds under 2° C. to 4° C. for a second stratification treatment to obtain ruptured seeds, the ruptured seeds are rinsed with water every 6-8 days throughout the second stratification treatment of a total duration of 115-125 days.
17. The method for breaking dormancy of Thesium chinense Turcz seeds as claimed in claim 14 , wherein in the step of placing the seeds under 2° C. to 4° C. for a second stratification treatment to obtain ruptured seeds, the ruptured seeds are rinsed with water every 6-8 days throughout the second stratification treatment of a total duration of 115-125 days.
18. The method for breaking dormancy of Thesium chinense Turcz seeds as claimed in claim 1 , wherein in the step of placing the seeds under 2° C. to 4° C. for a second stratification treatment to obtain ruptured seeds, the ruptured seeds are rinsed with water every 6-8 days throughout the second stratification treatment of a total duration of 115-125 days.
19. The method for breaking dormancy of Thesium chinense Turcz seeds as claimed in claim 18 , wherein in the step of placing the seeds under 2° C. to 4° C. for a second stratification treatment to obtain ruptured seeds, the ruptured seeds are rinsed with water every 7 days throughout the second stratification treatment of the total duration of 120 days.
20. The method for breaking dormancy of Thesium chinense Turcz seeds as claimed in claim 1 , wherein the step of keeping the seeds moist comprises placing the seeds on a mesh after removing the green skins and the stalks, and covering with a plastic film to keep the seeds moist.