Non-enzymatic debriding agent and method of use thereof
A non-enzymatic debridement agent that is less expensive, less cytotoxic, and more effective than enzymatic and sharp surgical debridement of eschar material. The non-enzymatic debridement agent is based on a formulation of detergent with a high critical micelle concentration and a denaturing agent along with an optional optimized buffer. The non-enzymatic wound debriding agent is able to dissolve eschar material in under one week. The buffer or ointment may contain an optimized pH, salt concentration, reducing agent, and other components that aid in the debridement of eschar material from chronic wounds and burn wounds. The non-enzymatic debridement agent can be formulated as an ointment, liquid rinse under pressure, and/or as a combination product with an advanced wound dressing that can act as a sink to remove the solubilized eschar material from the surface of the wound to assist in the debridment and proper healing of wounds.
1. A non-enzymatic wound debriding agent for application to necrotic human tissue that includes eschar that develops slough, comprising:
100-500 mM urea;
a gentle detergent with a high critical micelle concentration (CMC) of greater than 19 mM, wherein the micelles have hydrophilic denaturing cores that include the urea;
sodium chloride;
a physiological buffer; and
water.
2. The non-enzymatic wound debriding agent of claim 1 , wherein the detergent comprises a glycosidic headgroup comprising one or more of alkyl β-glucosides and alkyl β-maltosides, with alkyl chain lengths ranging from 8 to 14.
3. The non-enzymatic wound debriding agent of claim 1 , wherein the detergent comprises a peptide based peptergent that has detergent like properties.
4. The non-enzymatic wound debriding agent of claim 1 , wherein the detergent comprises a glycosidic headgroup comprising one or more of alkyl β-glucosides and alkyl β-maltosides, with alkyl chain lengths ranging from 8 to 14, and a peptide based peptergent that has detergent like properties.
5. The non-enzymatic wound debriding agent of claim 1 , wherein the detergent is a HECAMEG detergent.
6. The non-enzymatic wound debriding agent of claim 1 , wherein the buffer comprises one or more of N-2-aminoethanesulfonic acid (ACES), Phosphate Buffered Saline (PBS), and 2-(N-morpholino) ethanesulfonic acid (MES) at a concentration of 10-200 mM.
7. The non-enzymatic wound debriding agent of claim 1 , in a gel or ointment.
8. The non-enzymatic wound debriding agent of claim 1 , that is part of an antimicrobial and antibiotic solution, gel, or ointment, where the gel or ointment comprises xanthan gum, polyethylene glycol, or is petroleum based.
9. The non-enzymatic wound debriding agent of claim 8 , where the solution, gel or ointment further comprises a non mercaptan reducing agent that comprises a peptide or peptide like molecule.
10. The non-enzymatic wound debriding agent of claim 9 , where the detergent comprises a non mercaptan reducing disulfide moiety.
11. A method of debriding a wound, comprising:
applying to the wound a non-enzymatic wound debriding agent that comprises:
a micelle formulation comprising 100-500 mM urea;
a gentle detergent with a high critical micelle concentration (CMC) of greater than 19 mM, wherein the micelles have hydrophilic denaturing cores that include the urea;
sodium chloride;
a physiological buffer; and
water.
12. The method of claim 11 , wherein the detergent comprises a glycosidic headgroup comprising one or more of alkyl β-glucosides and alkyl β-maltosides, with alkyl chain lengths ranging from 8 to 14.
13. The method of claim 11 , wherein the detergent comprises a peptide based peptergent that has detergent like properties.
14. The method of claim 11 , wherein the detergent comprises a glycosidic headgroup comprising one or more of alkyl β-glucosides and alkyl β-maltosides, with alkyl chain lengths ranging from 8 to 14, and a peptide based peptergent that has detergent like properties.
15. The method of claim 11 , wherein the detergent is a HECAMEG detergent having a CMC of greater than 19 mM, in the D configuration to inhibit proteolysis during dissolution of eschar material.
16. The method of claim 11 , wherein the buffer comprises one or more of N-2-aminoethanesulfonic acid (ACES), Phosphate Buffered Saline (PBS), and 2-(N-morpholino) ethanesulfonic acid (MES) at a concentration of 10-200 mM.
17. The method of claim 11 , wherein the wound debriding agent is in a gel or ointment.
18. The method of claim 11 , wherein the wound debriding agent is part of an antimicrobial and antibiotic solution, gel, or ointment, where the gel or ointment comprises xanthan gum, polyethylene glycol, or is petroleum based.
19. The method of claim 18 , wherein the solution, gel or ointment further comprises a non mercaptan reducing agent that comprises a peptide or peptide like molecule.
20. The method of claim 19 , wherein the detergent comprises a non mercaptan reducing disulfide moiety.