Granted Patent B2

US 12,403,467 · App. 18/086,347 · Granted Sep 2, 2025

Inventors: Dustin Blair (Escondido, CA); Xiangxiong Chen (Palo Alto, CA); Gabriel Chan (San Diego, CA); Bindya Rani Dhanekula (La Jolla, CA); John Earney (San Diego, CA); Damian Kelley (San Mateo, CA)

Assignee: ILLUMINA, INC.

B01L3/502715G01J5/80B01L2300/0877B01L2300/1894

Abstract

Claims (45)

1. An apparatus, comprising:

a flow cell interface including a flow cell support adapted to have a flow cell positioned thereon;

a temperature control device for the flow cell support;

an infrared sensor;

a stage assembly configured to move the flow cell interface relative to the infrared sensor; and

a controller to command the temperature control device to cause the flow cell support to achieve a temperature value, cause the infrared sensor to measure an actual temperature value of the flow cell support, and calibrate the temperature control device based on a difference between the commanded temperature value and the actual temperature value.

2. The apparatus of claim 1 , wherein the temperature control device comprises a thermoelectric cooler.

3. The apparatus of claim 2 , wherein the temperature control device further comprises a resistance temperature detector.

4. The apparatus of claim 3 , wherein the controller is to calibrate the temperature control device by calibrating the resistance temperature detector.

5. The apparatus of claim 1 , further comprising an imaging system, the infrared sensor coupled to the imaging system.

6. The apparatus of claim 1 , wherein the controller is further to calibrate the infrared sensor.

7. The apparatus of claim 1 , further comprising a temperature sensor; wherein the infrared sensor is to measure a first temperature value adjacent to the temperature sensor; the temperature sensor is to measure a second temperature value; and the controller is to calibrate the infrared sensor based on a difference between the first temperature value and the second temperature value.

8. The apparatus of claim 7 , wherein the flow cell interface further comprises a flow cell deck carrying the flow cell support, the temperature sensor coupled to the flow cell deck.

9. The apparatus of claim 8 , further comprising a printed circuit board coupled to the temperature sensor, the printed circuit board positioned between the temperature sensor and the flow cell deck.

10. The apparatus of claim 7 , further comprising a metallic layer covering a surface of the temperature sensor.

11. The apparatus of claim 1 , further comprising a temperature sensor; wherein the infrared sensor is to measure a first temperature value of the flow cell support; the temperature sensor is to measure a second temperature value at the flow cell support; and the controller is to calibrate the infrared sensor based on a difference between the first temperature value and the second temperature value.

12. The apparatus of claim 11 , further comprising a printed circuit board coupled to the temperature sensor, the temperature sensor positioned between the flow cell interface and the printed circuit board.

13. The apparatus of claim 1 , wherein a surface of the flow cell support comprises a metallic layer.

14. An apparatus, comprising:

a flow cell interface including a flow cell support adapted to have a flow cell positioned thereon;

a temperature control device for the flow cell support;

an infrared sensor;

a stage assembly configured to move the flow cell interface relative to the infrared sensor; and

a controller to command the temperature control device to cause the flow cell support to achieve a temperature value, and cause the infrared sensor to measure a plurality of actual temperature values of the flow cell support.

15. The apparatus of claim 14 , wherein the controller is to generate a heat map based on the plurality of actual temperature values measured.

16. The apparatus of claim 15 , wherein the controller is to diagnosis an error based on the heat map.

17. The apparatus of claim 15 , wherein the controller is to calibrate the temperature control device based on the heat map.

18. The apparatus of claim 15 , wherein the heat map comprises a 2-D heat map.

19. An apparatus, comprising:

a flow cell interface including a flow cell support adapted to support a flow cell;

a temperature control device for the flow cell support;

an infrared sensor;

a temperature sensor;

a stage assembly configured to move the flow cell interface relative to the infrared sensor; and

a controller,

wherein the infrared sensor is to measure a first temperature value adjacent to the temperature sensor; the temperature sensor is to measure a second temperature value; and the controller is to calibrate the infrared sensor based on a difference between the first temperature value and the second temperature value.

20. The apparatus of claim 19 , further comprising a flow cell carrying the temperature sensor.

21. The apparatus of claim 20 , wherein the flow cell comprises a mock flow cell.

22. An apparatus, comprising:

a flow cell interface including a flow cell support adapted to have a flow cell positioned thereon;

a temperature control device for the flow cell support;

an infrared sensor; and

a controller to command the temperature control device to cause the flow cell support to achieve a temperature value, cause the infrared sensor to measure an actual temperature value of the flow cell support, and calibrate the temperature control device based on a difference between the commanded temperature value and the actual temperature value,

wherein the flow cell interface or the infrared sensor are configured to move relative to the other of the flow cell interface or the infrared sensor.

23. The apparatus of claim 22 , further comprising a stage assembly configured to move the flow cell interface relative to the infrared sensor.

Assignments (1)

Continuity (2)

References Cited (51)