Methods for the preparation of a plant protein composition
The present invention provides methods for the preparation of protein base compositions using antioxidants with heat treatment.
1. A method for preparing a protein base composition, comprising:
i) extracting a composition comprising one or more plant proteins from a source thereof, in an aqueous solution to provide an aqueous composition;
ii) heating the aqueous composition to a temperature of 260° F.-300° F. in the presence of one or more anti-oxidant compositions, wherein the one or more anti-oxidant compositions are at least one water soluble anti-oxidant composition and at least one lipid soluble anti-oxidant composition; and
iii) isolating a protein-containing fraction from the aqueous composition to provide a protein base composition, optionally using ultrafiltration or microfiltration,
wherein said method is performed without the addition of calcium salts,
wherein the plant protein source is a pulse protein.
2. The method of claim 1 , wherein the pulse protein is selected from pea and/or chickpea.
3. The method of claim 1 , wherein i) and iii) are carried out at a temperature of not more than 100° F., optionally, not more than 85° F.
4. The method of claim 1 , wherein ii) is carried out for a duration of time between 1-300 seconds.
5. The method of claim 1 , wherein the one or more anti-oxidants is one or more vitamins.
6. The method of claim 1 , wherein the one or more anti-oxidant compositions is 1% to 0.01% w/w of the total aqueous composition.
7. The method of claim 1 , wherein the pH of the aqueous composition is maintained at about 6-8.
8. The method of claim 1 , further comprising separating the aqueous plant protein solution from an insoluble plant protein source prior to ii).
9. The method of claim 1 , further comprising formulating the protein base composition into a food or beverage composition.
10. The method of claim 1 , wherein the protein base composition does not contain animal, soy, gluten, dairy matter and/or combinations thereof.
11. The method of claim 5 , wherein the one or more vitamins is Vitamin C and/or Vitamin E.
12. The method of claim 1 , wherein at least 80% or more particles of the protein base composition have a maximum dimension equal to or less than 1 um.
13. The method of claim 1 , wherein the at least one water soluble anti-oxidant composition is Vitamin C and the at least one lipid soluble anti-oxidant composition is Vitamin E.