Granted Patent
B2
US 12,435,452 · App. 17/411,590 · Granted Oct 7, 2025
Enzyme extraction methods
Inventors:
Edward Kavazanjian (Tempe, AZ); Nasser Hamdan (Scottsdale, AZ); Hamed Khodadadi Tirkolaei (Tempe, AZ); Neda Javadi (Tempe, AZ)
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Abstract
Disclosed are methods of extracting an enzyme, comprising soaking a seed in an aqueous solution, homogenizing the seed to produce a homogenized suspension, and filtering the homogenized suspension to produce a crude extract, wherein the crude extract comprises urease and non-urease proteins.
Claims (12)
1. A method of producing a powdered crude extract for biocementation, the method consisting of:
soaking a seed in an aqueous solution for between 12 and 24 hours to form a soaked seed, wherein the seed comprises jack bean, and wherein the aqueous solution consists of water;
subsequently homogenizing the soaked seed to produce a homogenized suspension with urease proteins and non-urease proteins;
filtering the homogenized suspension to produce a crude extract, wherein a purification fold of the crude extract is equal to 1; and
lyophilizing and/or drying the crude extract to produce the powdered crude extract, and wherein:
the powdered crude extract comprises urease proteins and non-urease proteins, and
the powdered crude extract is produced for a biocementation process for enzyme induced carbonate precipitation (EICP) treatment of soil.
2. The method of claim 1 , wherein the filtering comprises:
passing the homogenized suspension through a first filter to produce a solution; and
passing the solution through a second filter to produce the crude extract.
3. The method of claim 2 , wherein the second filter comprises glass wool.
4. The biocementation process of claim 1 , further comprising adding urea, a source of calcium ions, and an enzyme stabilizer to the powdered crude extract to produce a biocementation solution for the EICP treatment of the soil.
Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Aug 25, 2021
From: KAVAZANJIAN, EDWARD; TIRKOLAEI, HAMED KHODADADI; JAVADI, NEDA; HAMDAN, NASSER
To: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
Reel/Frame 057286/0214 →
Continuity (3)
Continuation
PCTUS2020019890
· Feb 26, 2020
Provisional Application
62810443
· Feb 26, 2019
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