Granted Patent B2

US 12,436,077 · App. 17/794,580 · Granted Oct 7, 2025

Inventors: Damiano Genovese (Bologna, IT); Luca Prodi (Bologna, IT); Enrico Rampazzo (Bologna, IT); Nelsi Zaccheroni (Bologna, IT)

Assignee: ALMA MATER STUDIORUM—UNIVERSITA' DI BOLOGNA

G01N15/06G01N31/22G01N33/20G01N15/075

Abstract

Claims (14)

1. A method for detecting nano- and micro-plastics in an aqueous sample suspected of being polluted with nano- or micro-plastics, the method comprising:

contacting the aqueous sample suspected of being polluted with nano- or micro-plastics with an aqueous solution of hyaluronic acid functionalized with a luminescent dye as a sensing probe, thereby obtaining a test sample;

illuminating the test sample with a light source configured to excite the luminescent dye;

measuring a luminescence parameter obtained from the test sample upon illumination, wherein the luminescence parameter is selected from luminescence intensity and luminescence lifetime; and

comparing the luminescence parameter obtained from the test sample with a same luminescence parameter obtained from a background sample by either a wide field microscopy method or a confocal microscopy method, wherein the background sample comprises the aqueous sample suspected of being polluted with nano- or micro-plastics in an absence of the sensing probe, wherein an increase in luminescence intensity or a variation in the luminescence lifetime of the test sample as compared to the background sample indicates a presence of the nano- or micro-plastics in the aqueous sample.

2. The method of claim 1 , wherein the aqueous sample is seawater or a biological sample.

3. The method of claim 1 , wherein the nano- or micro-plastics are selected from the group consisting of polystyrene (PS), polymethylmethacrylate (PMMA), polypropylene (PP) and any combination thereof.

4. The method of claim 1 , wherein the hyaluronic acid has a molecular weight varying from 8 to 1500 kDa.

5. The method of claim 1 , wherein the luminescent dye is selected from the group consisting of rhodamines, luminescent metallorganic complexes, xantenes, bodipys, coumarins, perylenes and cyanines.

6. The method of claim 5 , wherein the luminescent dye is Rhodamine B or Ru(bpy) 3 2+ .

7. The method of claim 1 , wherein the luminescence parameter is the luminescence lifetime and an increase in the luminescence lifetime obtained from the test sample as compared to the background sample is indicative of the presence of polymethylmethacrylate (PMMA) nano- or micro-plastics in the aqueous sample.

8. The method of claim 1 , wherein the luminescence parameter is the luminescence lifetime and a decrease in the luminescence lifetime obtained from the test sample as compared to the background sample is indicative of the presence of polystyrene (PS) or polypropylene (PP) nano- or micro-plastics in the aqueous sample.

9. The method of claim 1 , wherein the hyaluronic acid has a molecular weight varying from 150 to 500 kDa.

10. The method of claim 1 , wherein the hyaluronic acid has a molecular weight varying from 150 to 195 kDa.

Assignments (1)

Priority Claims (1)

Continuity (1)

References Cited (9)