IP Library Granted Patent US 12,441,985
Granted Patent B2
US 12,441,985 · App. 18/099,852 · Granted Oct 14, 2025

Methods for making insulin in vivo

Inventor: Jean Xu (Skillman, NJ)
Assignee: Janssen Biotech, Inc.
C12N5/0676C12N2501/117C12N2501/119C12N2501/16C12N2501/385C12N2501/40C12N2501/41C12N2501/415C12N2501/70C12N2501/727C12N2506/02C12N2533/90
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Quick Facts
Patent No.
US 12,441,985
App. No.
18/099,852
Granted
Oct 14, 2025
Kind
B2
Abstract

The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method to produce cells expressing markers characteristic of the pancreatic endocrine lineage that co-express NKX6.1 and insulin and minimal amounts of glucagon.

Claims (13)

1. A method of producing insulin in a mammalian subject, comprising:

transplanting, into the mammalian subject, clusters and/or aggregates of human pancreatic endoderm cells in a medium, wherein the human pancreatic endoderm cells differentiate into a population comprising NKX6.1 and insulin co-expressing human pancreatic endocrine cells, and wherein less than 10% of the cells in the population express glucagon,

thereby producing insulin in the mammalian subject.

2. The method of claim 1 , wherein at least 30% of the human pancreatic endocrine cells in the population express NKX6.1.

3. The method of claim 1 , wherein at least 40% of the human pancreatic endocrine cells in the population express NKX6.1.

4. The method of claim 1 , wherein at least 50% of the human pancreatic endocrine cells in the population express NKX6.1.

5. The method of claim 1 , wherein at least 60% of the human pancreatic endocrine in the population cells express NKX6.1.

6. The method of claim 1 , wherein at least 5% of the human pancreatic endocrine cells in the population express insulin.

7. The method of claim 1 , wherein the medium comprises noggin.

8. The method of claim 1 , wherein the subject has diabetes.

9. The method of claim 8 , wherein the diabetes is Type 1 diabetes.

10. The method of claim 1 , wherein the subject is human.

11. The method of claim 1 , wherein the human pancreatic endocrine cells produce insulin in response to glucose stimulation.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 23, 2023
From: XU, JEAN
To: CENTOCOR ORTHO BIOTECH, INC.
Reel/Frame 062458/0352 →
CHANGE OF NAME Recorded Jan 23, 2023
From: CENTOCOR ORTHO BIOTECH, INC.
To: JANSSEN BIOTECH, INC.
Reel/Frame 062474/0876 →
Continuity (6)
Continuation 16892106 · Jun 3, 2020
Continuation 15457956 · Mar 13, 2017
Continuation 14840297 · Aug 31, 2015
Continuation 12970330 · Dec 16, 2010
Provisional Application 61289671 · Dec 23, 2009
Related Publication 20230151332A1 · May 18, 2023
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