Sweeteners
The present disclosure relates, at least in part, to compositions (e.g., formulations) comprising a steviol glycoside. Rebaudioside M (Reb M), combined with brazzein that have improved caloric profile as well as flavor, taste and/or mouthfeel. In some embodiments, the composition further comprises one or more additional sweeteners.
1. A composition comprising rebaudioside M (Reb M) and brazzein, wherein brazzein is present in the composition in an amount that is between 1 ppm and 100 ppm, and wherein Reb M is present in the composition in an amount that is between 1 ppm and 600 ppm, further comprising one or more of rebaudioside A (Reb A), rebaudioside B (Reb B), rebaudioside D (Reb D), rebaudioside E (Reb E), rebaudioside I (Reb I), or combinations thereof.
2. The composition of claim 1 , further comprising Reb D.
3. The composition of claim 2 , wherein Reb D is present in the composition in an amount that is between 1 ppm and 500 ppm.
4. The composition of claim 1 , further comprising Reb E.
5. The composition of claim 4 , wherein Reb E is present in the composition in an amount that is between 1 ppm and 100 ppm.
6. The composition of claim 1 , further comprising Reb I.
7. The composition of claim 6 , wherein Reb I is present in the composition in an amount that is between 1 ppm and 100 ppm.
8. The composition of claim 1 , further comprising Reb B.
9. The composition of claim 8 , wherein Reb B is present in the composition in an amount that is between 1 ppm and 200 ppm.
10. The composition of claim 1 , further comprising Reb A.
11. The composition of claim 10 , wherein Reb A is present in the composition in an amount that is between 1 ppm and 200 ppm.
12. The composition claim 1 , wherein the composition has a sweetness intensity equivalent to the sweetness intensity of a solution comprising 1% to 15% (w/v-%) sucrose.
13. The composition of claim 1 , wherein the composition comprises brazzein and Reb M, wherein brazzein is present in the composition in an amount that is between 1 ppm and 30 ppm and Reb M is present in the composition in an amount that is between 250 ppm and 600 ppm.
14. The composition of claim 13 , wherein:
(i) brazzein is present in the composition in an amount that is between 4 ppm and 20 ppm and Reb M is present in the composition in an amount that is between 380 ppm and 450 ppm;
(ii) brazzein is present in the composition in an amount that is between 4 ppm and 20 ppm and Reb M is present in the composition in an amount that is between 480 ppm and 550 ppm;
(iii) brazzein is present in the composition in an amount that is between 4 ppm and 20 ppm and Reb M is present in the composition in an amount that is between 440 ppm and 540 ppm;
(iv) brazzein is present in the composition in an amount that is between 5 ppm and 25 ppm and Reb M is present in the composition in an amount that is between 280 ppm and 380 ppm;
(v) brazzein is present in the composition in an amount that is between 7 ppm and 14 ppm and Reb M is present in the composition in an amount that is between 400 ppm and 450 ppm;
(vi) brazzein is present in the composition in an amount that is between 7 ppm and 14 ppm and Reb M is present in the composition in an amount that is between 500 ppm and 550 ppm;
(vi) brazzein is present in the composition in an amount that is about 7 ppm and Reb M is present in the composition in an amount that is about 493 ppm; or
(vii) brazzein is present in the composition in an amount that is about 25 ppm and Reb M is present in the composition in an amount that is about 320 ppm.
15. The composition of claim 13 , wherein the composition has a sweetness intensity greater than the sweetness intensity of an otherwise identical composition except for the absence of brazzein.
16. The composition of claim 1 , wherein the composition comprises brazzein, Reb M, and Reb D, wherein brazzein is present in the composition in an amount that is between 1 ppm and 50 ppm, Reb M is present in the composition in an amount that is between 200 ppm and 500 ppm, and Reb D is present in the composition in an amount that is between 20 ppm and 200 ppm.
17. The composition of claim 16 , wherein:
(i) brazzein is present in the composition in an amount that is between 3 ppm and 30 ppm, Reb M is present in the composition in an amount that is between 300 ppm and 400 ppm, and Reb D is present in the composition in an amount that is between 60 ppm and 140 ppm; or
(ii) wherein brazzein is present in the composition in an amount that is between 4 ppm and 20 ppm, Reb M is present in the composition in an amount that is between 300 ppm and 400 ppm, and Reb D is present in the composition in an amount that is between 50 ppm and 150 ppm.
18. The composition of claim 16 , wherein the composition has a sweetness intensity equivalent to the sweetness intensity of a solution comprising 200 ppm sucralose.
19. The composition of claim 1 , wherein the composition comprises brazzein, Reb M, Reb D, Reb E, and Reb I, wherein brazzein is present in the composition in an amount that is between 1 ppm and 40 ppm, Reb M is present in the composition in an amount that is between 1 ppm to 500 ppm, Reb D is present in the composition in an amount that is between 1 ppm and 350 ppm, Reb E is present in the composition in an amount that is between 1 ppm and 100 ppm, and Reb I is present in the composition in an amount that is between 1 ppm and 100 ppm.
20. The composition of claim 19 , wherein:
(i) brazzein is present in the composition in an amount that is between 1 ppm and 20 ppm, Reb D is present in the composition in an amount that is between 1 ppm to 10 ppm, Reb E is present in the composition in an amount that is between 1 ppm and 20 ppm, Reb M is present in the composition in an amount that is between 1 ppm and 14 ppm, and Reb I is present in the composition in an amount that is between 1 ppm and 7 ppm;
(ii) brazzein is present in the composition in an amount that is between 4 ppm and 30 ppm, Reb M is present in the composition in an amount that is between 300 ppm to 400 ppm, Reb D is present in the composition in an amount that is between 30 ppm and 80 ppm, Reb E is present in the composition in an amount that is between 20 ppm and 80 ppm, and Reb I is present in the composition in an amount that is between 30 ppm and 80 ppm; optionally wherein the composition further comprises thaumatin at 1-5 ppm;
(iii) brazzein is present in the composition in an amount that is between 4 ppm and 20 ppm, Reb M is present in the composition in an amount that is between 250 ppm to 450 ppm, Reb D is present in the composition in an amount that is between 30 ppm and 80 ppm, Reb E is present in the composition in an amount that is between 20 ppm and 80 ppm, and Reb I is present in the composition in an amount that is between 30 ppm and 80 ppm;
(iv) brazzein is present in the composition in an amount that is between 4 ppm and 30 ppm, Reb M is present in the composition in an amount that is between 250 ppm to 500 ppm, Reb D is present in the composition in an amount that is between 25 ppm and 150 ppm, Reb E is present in the composition in an amount that is between 25 ppm and 75 ppm, and Reb I is present in the composition in an amount that is between 25 ppm and 75 ppm; optionally wherein the composition further comprises glycerin at 500 ppm and 4000 ppm; further optionally wherein the composition has a sweetness intensity level equivalent to sweetness intensity of a solution comprising 1-3% w/w of sucrose; or
(v) brazzein is present in the composition in an amount that is between 3 ppm and 30 ppm, Reb M is present in the composition in an amount that is between 100 ppm to 400 ppm, Reb D is present in the composition in an amount that is between 50 ppm and 300 ppm, Reb E is present in the composition in an amount that is between 20 ppm and 70 ppm, and Reb I is present in the composition in an amount that is between 20 ppm and 60 ppm; optionally wherein the composition further comprises malic acid at 0.1-0.4% w/w; further optionally wherein the composition has a sweetness intensity level equivalent to sweetness intensity of a solution comprising 1-3% w/w of sucrose.
21. The composition of claim 19 , wherein the composition has a sweetness intensity equivalent to the sweetness intensity of a solution comprising 1% to 15% (w/v-%) sucrose.
22. The composition of claim 1 , wherein the composition comprises brazzein, Reb M, Reb D, Reb E, Reb I, and Reb B, wherein brazzein is present in the composition in an amount that is between 4 ppm and 20 ppm, Reb M is present in the composition in an amount that is between 2 ppm to 400 ppm, Reb D is present in the composition in an amount that is between 5 ppm and 50 ppm, Reb E is present in the composition in an amount that is between 5 ppm and 50 ppm, Reb I is present in the composition in an amount that is between 5 ppm and 50 ppm, and Reb B is present in the composition in an amount that is between 5 ppm and 100 ppm.
23. The composition of claim 22 , further comprising one or more of caffeine, taurine, niacin (Vitamin B3), pyridoxine (Vitamin B6), pantothenic acid (Vitamin B5), cobalamin (Vitamin B12), and combination thereof; optionally wherein caffeine is present in the composition in an amount that is between 100 ppm-600 ppm, taurine is present in the composition in an amount that is between 500 ppm and 12000 ppm, niacin (Vitamin B3) is present in the composition in an amount that is between 5-680 ppm, pyridoxine (Vitamin B6) is present in the composition in an amount that is between 5-180 ppm, pantothenic acid (Vitamin B5) is present in the composition in an amount that is between 5-120 ppm, and cobalamin (Vitamin B12) is present in the composition in an amount that is between 5-80 ppm.
24. The composition of claim 1 , wherein the composition comprises brazzein, Reb M, Reb D, Reb E, Reb I, Reb B, and Reb A, wherein brazzein is present in the composition in an amount that is between 1 ppm and 50 ppm, Reb M is present in the composition in an amount that is between 50 ppm to 400 ppm, Reb D is present in the composition in an amount that is between 1 ppm and 350 ppm, Reb E is present in the composition in an amount that is between 5 ppm and 100 ppm, Reb I is present in the composition in an amount that is between 5 ppm and 100 ppm, Reb A is present in the composition in an amount that is between 1 ppm and 100 ppm, and Reb B is present in the composition in an amount that is between 1 ppm and 100 ppm.
25. The composition of claim 24 , wherein:
(i) brazzein is present in the composition in an amount that is between 3 ppm and 30 ppm, Reb M is present in the composition in an amount that is between 300 ppm to 400 ppm, Reb D is present in the composition in an amount that is between 60 ppm and 140 ppm, Reb E is present in the composition in an amount that is between 30 ppm and 70 ppm, Reb I is present in the composition in an amount that is between 30 ppm and 70 ppm, Reb A is present in the composition in an amount that is between 1 ppm and 10 ppm, and Reb B is present in the composition in an amount that is between 30 ppm and 70 ppm;
(ii) brazzcin is present in the composition in an amount that is between 7 ppm and 40 ppm, Reb M is present in the composition in an amount that is between 220 ppm to 300 ppm, Reb D is present in the composition in an amount that is between 2 ppm and 40 ppm, Reb E is present in the composition in an amount that is between 5 ppm and 50 ppm, Reb I is present in the composition in an amount that is between 5 ppm and 50 ppm, Reb A is present in the composition in an amount that is between 5 ppm and 100 ppm, and Reb B is present in the composition in an amount that is between 5 ppm and 100 ppm;
(iii) brazzein is present in the composition in an amount that is between 7 ppm and 40 ppm, Reb M is present in the composition in an amount that is between 220 ppm to 300 ppm, Reb D is present in the composition in an amount that is between 5 ppm and 300 ppm, Reb E is present in the composition in an amount that is between 5 ppm and 80 ppm, Reb I is present in the composition in an amount that is between 15 ppm and 50 ppm, Reb A is present in the composition in an amount that is between 5 ppm and 100 ppm, and Reb B is present in the composition in an amount that is between 5 ppm and 100 ppm; or
(iv) brazzein is present in the composition in an amount that is between 1 ppm and 40 ppm, Reb M is present in the composition in an amount that is between 50 ppm to 400 ppm, Reb D is present in the composition in an amount that is between 50 ppm and 300 ppm, Reb E is present in the composition in an amount that is between 5 ppm and 50 ppm, Reb I is present in the composition in an amount that is between 5 ppm and 50 ppm, Reb A is present in the composition in an amount that is between 5 ppm and 100 ppm, and Reb B is present in the composition in an amount that is between 5 ppm and 100 ppm; optionally wherein the composition further comprises malic acid at 0.05-0.35% (w/w) or citric acid at 0.05-0.4% (w/w); further optionally wherein the composition further comprises apple juice at 10-50% (v/v).
26. The composition of claim 24 , wherein the composition has a sweetness intensity greater than the sweetness intensity of an otherwise identical composition except for the absence of brazzein.
27. A consumable comprising the composition of claim 1 .
28. The consumable of claim 27 , wherein the consumable is an oral composition, a pharmaceutical composition, a beverage, a food composition, an animal feed product, a liquid, a powder, a table-top sweetener.
29. The consumable of claim 27 , wherein the consumable is cocoa mix, umami and/or savoury food, tomato ketchup, a sauce, a snack, an alcoholic beverage, fruit juice, fruit juice flavored drink, apple juice, carbonated apple juice, flavored water, enhanced water, acidified water, soft drink cola, or lemonade.