Blood separation by microfluidic acoustic focusing
Systems and methods for cleansing blood are disclosed herein. The methods include acoustically separating undesirable particles bound to capture particles from formed elements of whole blood. After introducing the capture particles to whole blood containing undesirable particles, the whole blood and capture particles are flowed through a microfluidic separation channel. At least one bulk acoustic transducer is attached to the microfluidic separation channel. A standing acoustic wave, imparted on the channel and its contents by the bulk acoustic transducer, drives the formed elements and undesirable particles bound to capture particles to specific aggregation axes. After aggregating the particles, the formed elements exit the separation channel through a first outlet and are returned to the patient. The undesirable particles, bound to the capture particles, exit through a second outlet and can be discarded to saved for later study.
1. A method of cleansing blood comprising:
flowing whole blood, including plasma, a plurality of formed elements, and a plurality of undesirable particles, into an inlet of a microfluidic separation channel defined in a thermoplastic;
selecting a wavelength of a standing acoustic wave such that a predetermined width of the microfluidic separation channel is between 30% and 45% of the wavelength of the standing acoustic wave; and
applying the standing acoustic wave transverse to a direction of flow of the whole blood through the microfluidic separation channel such that the plurality of formed elements aggregate toward the axial center of the microfluidic separation channel.
2. The method of claim 1 , wherein the predetermined width of the microfluidic separation channel is between about 30% and about 35% of the wavelength of the standing acoustic wave applied to the microfluidic separation channel.
3. The method of claim 1 , wherein a thickness of a wall of the microfluidic separation channel is between about 35% and about 45% of the wavelength of the standing acoustic wave applied to the microfluidic separation channel.
4. The method of claim 1 , further comprising introducing a plurality of lipid-based capture particles into the whole blood such that the plurality of lipid-based capture particles bind to the plurality of undesirable particles.
5. The method of claim 1 , further comprising collecting the plurality of formed elements of the whole blood at a first outlet positioned at a downstream end of the microfluidic separation channel at about the axial center of the microfluidic separation channel.
6. The method of claim 4 , further comprising collecting the plurality of lipid-based capture particles through at least a second outlet positioned at a downstream end of the microfluidic separation channel adjacent to at least one wall along which the plurality of lipid-based capture particles are aggregated.
7. The method of claim 4 , wherein the plurality of lipid-based capture particles comprise an affinity molecule anchored to a lipid bilayer encapsulating a fluid.
8. The method of claim 4 , wherein introducing the plurality of lipid-based capture particles into the whole blood comprises introducing the plurality of lipid-based capture particles using a capture particle injector before the whole blood reaches a particle migration region of the microfluidic separation channel.
9. The method of claim 4 , further comprising forming the plurality of lipid-based capture particles from a mixture of materials comprising an affinity molecule, a lipid, and a fluid with a density less than about 1 g/cm 3 .
10. The method of claim 9 , wherein forming the plurality of lipid-based capture particles comprises injecting the mixture of materials comprising the affinity molecule, the lipid, and the fluid through a nozzle such that the lipid forms a liposome surrounding a portion of the fluid.