Holographic imaging device and holographic imaging method
The present invention provides a holographic imaging device and a holographic imaging method that have improved performance in which the influence of a refractive index of a cube-type beam coupler constituting an optical system is considered. The holographic imaging device 1 comprises the beam coupler 3 consisting of the cube-type beam splitter arranged between the object 4 and the image sensor 5 and the calculation reference light hologram generation unit 14 for generating an inline reference light hologram j L representing a light wave on the hologram plane 50 by performing a light wave propagation calculation including propagation inside the beam coupler 3 , on a spherical wave emitted from the condensing point P 2 of the inline spherical wave reference light L. The inline reference light hologram j L is a computer-generated hologram and used for generating an object light hologram g by removing component of the reference light L from a complex-amplitude inline hologram J OL representing the object light O and the inline spherical wave reference light L on the hologram plane 50.
1. A holographic imaging device, comprising:
a data acquisition unit for acquiring data of an object light (O) emitted from an illuminated object and data of an inline spherical wave reference light (L) being inline with the object light (O), electronically and individually, as two kinds of off-axis holograms (I OR , I LR ) on a hologram plane defined at a light receiving surface of an image sensor, using an off-axis reference light (R) maintained under the same conditions; and
an image reconstruction unit for reconstructing an image of the object from the data acquired by the data acquisition unit, wherein
the data acquisition unit comprises:
a beam coupler consisting of a cube-type beam splitter, wherein the data acquisition unit acquires data of two lights of the object light (O) and the inline spherical wave reference light (L), passing through the beam coupler and being incident on the image sensor as the data of the two kinds of the off-axis holograms (I OR , I LR ), individually,
the image reconstruction unit comprises:
a complex amplitude hologram generation unit for generating a complex amplitude inline hologram (J OL ) on the hologram plane, containing information on both of the object light (O) and the inline spherical wave reference light (L), from the data of the two kinds of the off-axis holograms (I OR , I LR );
a calculation reference light hologram generation unit for generating an inline reference light hologram (j L ) representing a light wave of the inline spherical wave reference light (L) on the hologram plane by performing a light wave propagation calculation, based on the information on a position of a condensing point (P 2 ) of the inline spherical wave reference light (L), using a plane wave expansion method, including propagation calculation inside the beam coupler in consideration of refractive index of the beam coupler; and
an object light hologram generation unit for generating an object light hologram (g) being a hologram of the object light (O), on the hologram plane, by eliminating components of the inline spherical wave reference light (L) from the data of the complex amplitude inline hologram (J OL ) using the data of the inline reference light hologram (j L ).
2. The holographic imaging device according to claim 1 , wherein
the calculation reference light hologram generation unit calculates a phase (φ Lm ) of a spherical wave light on the hologram plane by performing a light wave propagation calculation from the condensing point (P 2 ) of the inline spherical wave reference light (L) to the hologram plane, using a plane wave expansion method, on a light having a converted wavelength (mλ) lengthened by multiplying the wavelength (λ) of the inline spherical wave reference light (L) by a coefficient (m), and generates the inline reference light hologram (j L ) by obtaining a phase (mφ Lm ) thereof, wherein the phase (mφ Lm ) thereof is obtained by multiplying the phase (φ Lm ) calculated from the light having the converted wavelength (mλ) by the coefficient (m).
3. The holographic imaging device according to claim 1 , wherein
the off-axis reference light (R) is a spherical wave-like light having a condensing point (P 1 ), and is incident on a side surface of the beam coupler, and
the condensing point (P 1 ) of the off-axis reference light (R) and a condensing point (P 2 ) of the inline spherical wave reference light (L) are optically close to each other.
4. The holographic imaging device according to claim 3 , wherein
the condensing point (P 1 ) of the off-axis reference light (R) and the condensing point (P 2 ) of the inline spherical wave reference light (L) are close to the beam coupler, and the holographic imaging device is used as a microscope.
5. The holographic imaging device according to claim 4 , wherein
the beam coupler is configured so that a thickness in an optical axis direction of the beam coupler, along which the object light (O) is incident, is thinner than a thickness in a lateral direction of the beam coupler, along which the off-axis reference light (R) is incident, and the condensing point (P 1 ) of the off-axis reference light (R) is inside the beam coupler.
6. The holographic imaging device according to claim 4 , wherein
the beam coupler has a chamfered portion formed so that the illumination light (Q) for illuminating the object from an oblique direction does not interfere with the beam coupler.
7. The holographic imaging device according to claim 4 , wherein
the illumination light (Q) for illuminating the object is incident on the beam coupler from a side surface of the beam coupler that is opposite to the side surface of the beam coupler on which the off-axis reference light (R) is incident, and the holographic imaging device is used as a reflection type microscope.
8. A holographic imaging method, comprising the steps of:
acquiring data of an object light (O), emitted from an illuminated object and propagating straight within a beam coupler consisting of a cube-type beam splitter and incident on an image sensor, as an object light off-axis hologram (I OR ) using an off-axis reference light (R) incident on the beam coupler from a side surface thereof, reflected therein, and incident on the image sensor;
acquiring data of the off-axis reference light (R) as a reference light off-axis hologram (I LR ) by the image sensor using an inline spherical wave reference light (L) being inline with the object light (O);
generating a complex amplitude inline hologram (J OL ) on a hologram plane defined at a light receiving surface of the image sensor from the data of the object light off-axis hologram (I OR ) and the reference light off-axis hologram (I LR );
generating an inline reference light hologram (j L ) representing a light wave of the inline spherical wave reference light (L) on the hologram plane by performing a light wave propagation calculation, based on the information on a position of a condensing point (P 2 ) of the inline spherical wave reference light (L), using a plane wave expansion method, on a spherical wave emitted from the condensing point (P 2 ) of the inline spherical wave reference light (L), including propagation calculation inside the beam coupler in consideration of refractive index of the beam coupler; and
generating an object light hologram (g) representing a hologram of the object light (O) on the hologram plane, by eliminating components of the inline spherical wave reference light (L) from the data of the complex amplitude inline hologram (J OL ) of the object light using the data of the inline reference light hologram (j L ).
9. The holographic imaging method according to claim 8 , wherein
the light wave propagation calculation for calculating the inline reference light hologram (j L ) is performed
by calculating a phase (φ Lm ) of a spherical wave light on the hologram plane by performing a light wave propagation calculation from the condensing point (P 2 ) of the inline spherical wave reference light (L) to the hologram plane, using a plane wave expansion method, on a light having a converted wavelength (mx) lengthened by multiplying the wavelength (λ) of the inline spherical wave reference light (L) by a coefficient (m), and
by generating the inline reference light hologram (j L ) by obtaining a phase (mφ Lm ) thereof by multiplying the phase (φ Lm ) calculated from the light having the converted wavelength (mλ) by the coefficient (m).
10. The holographic imaging method according to claim 8 , wherein
a distance (ρ) from the image sensor to the condensing point (P 2 ) of the inline spherical wave reference light (L) is used for calculating the inline reference light hologram (j L ), wherein the distance (ρ) is derived by:
acquiring data of a target object light (O T ), being a transmitted light of the inline spherical wave reference light (L) irradiating a target consist of a scale pattern on a transparent plate arranged at the position of and instead of the object, as a target off-axis hologram (I TR ) using the off-axis reference light (R);
generating the inline reference light hologram (j L ) using the distance (ρ) as a parameter;
generating a target object light hologram (gr) representing an object light of the target on the hologram plane using the target off-axis hologram (I TR ), the reference light off-axis hologram (I LR ), and the inline reference light hologram (j L );
reconstructing an image of the target at a position of the target by performing a light wave propagation calculation, for a position transformation, on the target object light hologram (gr);
determining the distance (ρ) as a value of the parameter when a size of the reconstructed image of the target matches the size of the target.
11. The holographic imaging method according to claim 9 , wherein
a distance (ρ) from the image sensor to the condensing point (P 2 ) of the inline spherical wave reference light (L) is used for calculating the inline reference light hologram (j L ), wherein the distance (ρ) is derived by:
acquiring data of a target object light (O T ), being a transmitted light of the inline spherical wave reference light (L) irradiating a target consist of a scale pattern on a transparent plate arranged at the position of and instead of the object, as a target off-axis hologram (I TR ) using the off-axis reference light (R);
generating the inline reference light hologram (j L ) using the distance (ρ) as a parameter;
generating a target object light hologram (gr) representing an object light of the target on the hologram plane using the target off-axis hologram (I TR ), the reference light off-axis hologram (I LR ), and the inline reference light hologram (j L );
reconstructing an image of the target at a position of the target by performing a light wave propagation calculation, for a position transformation, on the target object light hologram (gr);
determining the distance (ρ) as a value of the parameter when a size of the reconstructed image of the target matches the size of the target.
12. The holographic imaging device according to claim 2 , wherein
the off-axis reference light (R) is a spherical wave-like light having a condensing point (P 1 ), and is incident on a side surface of the beam coupler, and
the condensing point (P 1 ) of the off-axis reference light (R) and a condensing point (P 2 ) of the inline spherical wave reference light (L) are optically close to each other.
13. The holographic imaging device according to claim 12 , wherein
the condensing point (P 1 ) of the off-axis reference light (R) and the condensing point (P 2 ) of the inline spherical wave reference light (L) are close to the beam coupler, and the holographic imaging device is used as a microscope.
14. The holographic imaging device according to claim 13 , wherein
the beam coupler is configured so that a thickness in an optical axis direction of the beam coupler, along which the object light (O) is incident, is thinner than a thickness in a lateral direction of the beam coupler, along which the off-axis reference light (R) is incident, and the condensing point (P 1 ) of the off-axis reference light (R) is inside the beam coupler.
15. The holographic imaging device according to claim 14 , wherein
the beam coupler has a chamfered portion formed so that the illumination light (Q) for illuminating the object from an oblique direction does not interfere with the beam coupler.
16. The holographic imaging device according to claim 13 , wherein
the beam coupler has a chamfered portion formed so that the illumination light (Q) for illuminating the object from an oblique direction does not interfere with the beam coupler.
17. The holographic imaging device according to claim 5 , wherein
the beam coupler has a chamfered portion formed so that the illumination light (Q) for illuminating the object from an oblique direction does not interfere with the beam coupler.
18. The holographic imaging device according to claim 13 , wherein
the illumination light (Q) for illuminating the object is incident on the beam coupler from a side surface of the beam coupler that is opposite to the side surface of the beam coupler on which the off-axis reference light (R) is incident, and the holographic imaging device is used as a reflection type microscope.