Sample vessel having a cavity for accommodating a sample
The disclosure relates to a sample vessel including a cavity for accommodating a sample, a first opening for introducing the sample and a bottom opposite the first opening as well as at least one additional opening of the cavity. According to the disclosure, the at least one additional opening opens toward the outside of the cavity on the side of the cavity opposing the bottom. The disclosure further relates to a carrier plate, an evacuation needle, as well as a method for evacuating a sample.
1 . A sample vessel comprising:
a sample vessel body defining a central cavity, wherein said cavity is arranged to receive and immobilize a single cell and to this end includes:
a first opening for introducing said single cell, whereas said opening is of a round or a hexagonal cross-section that in size matches said single cell;
said sample vessel body having a bottom opposite said first opening;
said sample vessel body further defining two additional openings on two opposing edges of said first opening of the cavity;
wherein said two additional openings each open to outside of said cavity on a side of the cavity opposite said bottom; and,
wherein said two additional openings are smaller in diameter than said single cell.
2 . The sample vessel of claim 1 , wherein said sample vessel body defines a channel connected to each said two additional openings and said channel opens into said cavity.
3 . The sample vessel of claim 1 , wherein said two additional openings lie substantially in a plane with said first opening and are connected to said first opening.
4 . The sample vessel of claim 1 , wherein said first opening has an inner diameter (A) of no more than 0.5 millimeters.
5 . The sample vessel of claim 1 , wherein said first opening has an inner diameter (A) of no more than 0.3 millimeters.
6 . A carrier plate comprising a plurality of sample vessels according to claim 1 .
7 . A method for evacuating a single cell from a cavity of a sample vessel, the method comprising:
directing a first flow of a medium into the cavity and directing a second flow of the medium out of the cavity, wherein the flows are generated by using at least two channels separately opening into the cavity;
generating the first flow and the second flow by causing pressure differences in the at least two channels; and,
adjusting at least one of the first flow and the second flow and a transporting capacity thereof so as to cause said single cell to be flushed out of the cavity.
8 . A method for evacuating a single cell sample from a cavity of each sample vessel of a plurality of sample vessels with a carrier plate including the plurality of sample vessels, each of the sample vessels including: a sample vessel body defining a central cavity, wherein the cavity is arranged to receive and immobilize a single cell and to this end includes: a first opening for introducing the single cell, whereas the opening is of a round or a hexagonal cross-section that in size matches the single cell; the sample vessel body having a bottom opposite the first opening; the sample vessel body further defining two additional openings on two opposing edges of the first opening of the cavity; wherein the two additional openings each open to outside of the cavity on a side of the cavity opposite the bottom; and, wherein the two additional openings are smaller in diameter than the single cell; the method comprising the steps of:
providing an evacuation needle for selectively extracting a single cell from the cavity of each sample vessel of the carrier plate, the evacuation needle including: a needle body defining a tip and a main channel, wherein the needle body defines a secondary channel that is additionally provided;
opening the main channel and the secondary channel separately in an area of the tip of the evacuation needle; and,
wherein a first channel is in alignment with a first opening of the cavity of the sample vessel when a secondary channel is aligned with a second opening of the cavity of the sample vessel.