IP Library › Granted Patent US 12,459,875
Granted Patent B2
US 12,459,875 · App. 18/646,827 · Granted Nov 4, 2025

Use of phosphate-solubilizing microorganisms and lipo-chitooligosaccharides for enhancing plant growth

Inventors: Michael Frodyma (St. Louis, MO); Shawn Semones (St. Louis, MO); R. Stewart Smith (Pewaukee, WI); Yaowei Kang (Christiansburg, VA)
Assignee: NOVOZYMES BIOAG A/S
C05F11/08A01N43/16A01N63/36
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Quick Facts
Patent No.
US 12,459,875
App. No.
18/646,827
Granted
Nov 4, 2025
Kind
B2
Abstract

Methods for enhancing the growth and/or yield of plants, such as corn and wheat, through the application of one or more phosphate-solubilizing microorganisms and one or more lipo-chitooligosaccharides and/or chitooligosaccharides to plant seeds prior to planting.

Claims (20)

1 . A method comprising:

a) applying at least one lipo-chitooligosaccharide and at least one strain of Penicillium to a plant seed, thereby producing a treated plant seed; and

b) storing said treated plant seed for at least two months prior to planting said treated plant seed.

2 . The method of claim 1 , wherein said treated plant seed is stored for at least three months prior to planting said treated plant seed.

3 . The method of claim 1 , wherein said treated plant seed is stored for at least six months prior to planting said treated plant seed.

4 . The method of claim 1 , wherein said treated plant seed is stored for at least nine months prior to planting said treated plant seed.

5 . The method of claim 1 , wherein said treated plant seed is stored for at least twelve months prior to planting said treated plant seed.

6 . The method of claim 1 , wherein said treated plant seed is stored for at least eighteen months prior to planting said treated plant seed.

7 . The method of claim 1 , wherein said treated plant seed is stored for at least twenty-four months prior to planting said treated plant seed.

8 . The method of claim 1 , wherein said at least one lipo-chitooligosaccharide comprises an LCO represented by the structure:

9 . The method of claim 1 , wherein said at least one lipo-chitooligosaccharide comprises an LCO represented by the structure:

10 . The method of claim 1 , wherein said at least one lipo-chitooligosaccharide is applied to said plant seed at a concentration ranging from about 10 −14 to about 10 −5 M.

11 . The method of claim 1 , wherein said at least one strain of Penicillium comprises one or more strains of Penicillium bilaiae.

12 . The method of claim 1 , wherein said at least one strain of Penicillium comprises Penicillium bilaiae strain ATCC 20851, Penicillium bilaiae strain NRRL 50169, Penicillium bilaiae strain ATCC 22348, Penicillium bilaiae strain ATCC 18309, and/or Penicillium bilaiae strain NRRL B-50162.

13 . The method of claim 1 , wherein said at least one strain of Penicillium comprises at least two of Penicillium bilaiae strain ATCC 20851, Penicillium bilaiae strain NRRL 50169, Penicillium bilaiae strain ATCC 22348, Penicillium bilaiae strain ATCC 18309, and Penicillium bilaiae strain NRRL B-50162.

14 . The method of claim 1 , wherein said at least one strain of Penicillium comprises Penicillium bilaiae strain NRRL 50169 and Penicillium bilaiae strain NRRL B-50162.

15 . The method of claim 1 , wherein said at least one strain of Penicillium is applied to said plant seed at a rate of at least 1×10 7 colony forming units per gram of inoculant composition.

16 . The method of claim 1 , wherein said plant seed is a cereal seed.

17 . The method of claim 1 , wherein said plant seed is a corn seed.

18 . The method of claim 1 , wherein said plant seed is a wheat seed.

Assignments (1)
CHANGE OF NAME AND ADDRESS Recorded Oct 7, 2024
From: NOVOZYMES BIOAG A/S
To: NOVONESIS PLANT BIOSOLUTIONS A/S
Reel/Frame 070166/0088 →
Continuity (3)
Continuation 14345057
Provisional Application 61534639 · Sep 14, 2011
Related Publication 20240300868A1 · Sep 12, 2024
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