Granted Patent B2

US 12,461,013 · App. 17/711,390 · Granted Nov 4, 2025

Inventors: Paul Reed Smith (Annapolis, MD); William G. Nelson (Cockeysville, MD); Shane G. W. Morris (Baltimore, MD); Sandor Szalay (Baltimore, MD); Jonathan B. Coulter (Baltimore, MD); Frederick M. Slay (Okatie, SC); Eric K. Pritchard (Berkeley Springs, WV)

Assignee: Digital Harmonic LLC

G01N15/1484G01N15/1459G01R23/02G01R23/16G01N2015/1486G01N2015/1493G06F2218/10

Abstract

Claims (39)

1 . An optical device comprising:

a low-noise illumination source configured to generate a beam of radiation;

a support device configured to support an object and allow the beam of radiation to pass through the object, wherein the object directly blocks, absorbs, or deflects portions of the beam of radiation, thereby directly modulating the beam of radiation;

a low-noise detector configured to detect the modulated beam of radiation and to output an analog signal representative of vibrational spectra of the object, wherein the modulated beam of radiation is non-interferometric;

an analog-to-digital converter configured to convert the analog signal into a digital signal; and

a controller configured to generate a vibrational spectrum of the object from the digital signal represented as a range of events over a time period,

wherein the vibrational spectrum is in the sub-sonic frequency range.

2 . The optical device of claim 1 , wherein the optical device is configured to detect and measure the vibrational spectra of the object at or below a noise level produced by the low-noise illumination source, the low-noise detector, and the surrounding environment.

3 . The optical device of claim 1 , wherein the low-noise illumination source has a bandwidth in a range of 375 nm to 625 nm.

4 . The optical device of claim 3 , wherein the beam of radiation has a wavelength of 500 nm.

5 . The optical device of claim 1 , wherein the support device comprises a sound absorber configured to suppress frequencies below about 100 Hz.

6 . The optical device of claim 1 , wherein the support device comprises a heater configured to regulate the temperature of the object.

7 . The optical device of claim 1 , wherein the low-noise detector comprises a single pixel detector or a multiple pixel detector.

8 . The optical device of claim 1 , wherein the controller comprises a precision measuring matrix (PMM) configured to analyze and to filter the range of events into the vibrational spectrum.

9 . The optical device of claim 1 , wherein the object comprises living cells.

10 . The optical device of claim 9 , wherein at least one sub-cellular structure of the living cells comprises a fluorescent marker configured to emit radiation at a different wavelength than a wavelength of the beam of radiation.

11 . The optical device of claim 9 , wherein the living is cells are coupled to a slide.

12 . The optical device of claim 1 , wherein the low-noise illumination source, the low-noise detector, and/or the analog-to-digital converter comprises a DC battery power source or a low-noise DC power supply for noise reduction.

13 . The optical device of claim 1 , further comprising a second low-noise detector configured to detect a reference beam of radiation for noise reduction.

14 . A method for measuring low-noise vibrational spectra, the method comprising of:

generating a beam of radiation from a low-noise illumination source;

passing the beam of radiation through an object, wherein the object directly blocks, absorbs, or deflects portions of the beam of radiation, thereby directly modulating the beam of radiation;

detecting the modulated beam of radiation with a low-noise detector, wherein:

the low-noise detector outputs an analog signal representative of vibrational spectra of the object, and

the modulated beam of radiation is non-interferometric;

converting the analog signal into a digital signal with an analog-to-digital converter; and

generating a vibrational spectrum of the object from the digital signal represented as a range of events over a time period,

wherein the vibrational spectrum is in the sub-sonic frequency range.

15 . The method of claim 14 , wherein the generating the vibrational spectrum comprises measuring the vibrational spectra of the object at or below a noise level produced by the low-noise illumination source, the low-noise detector, and the surrounding environment.

16 . The method of claim 14 , wherein the generating the beam of radiation comprises providing a constant beam level in amplitude and a constant beam diameter.

17 . The method of claim 14 , further comprising aligning the object relative to the beam of radiation to optimize an amplitude of the analog signal.

18 . The method of claim 14 , further comprising heating the object to regulate the temperature of the object.

19 . The method of claim 14 , wherein the detecting comprises detecting the modulated beam of radiation with a single pixel detector or a multiple pixel detector.

20 . The method of claim 14 , wherein the generating the vibrational spectrum comprises filtering the range of events with a precision measuring matrix (PMM).

21 . The method of claim 20 , wherein the filtering comprises:

mapping the digital signal into a plurality of partials, each partial representing an amplitude over a specific frequency range and time period, and

chaining the plurality of partials into a plurality of events, each event representing a collection of partials localized over a specific frequency range and time period.

22 . The method of claim 14 , further comprising measuring a baseline noise measurement without the object.

23 . The method of claim 14 , wherein the object comprises living cells.

Assignments (1)

Continuity (4)

References Cited (42)