Compounds for use in system suitability testing of inert LC systems and columns
The present disclosure is directed to a kit for evaluating system inertness. The kit includes a positive control comprising a metal interacting moiety, and a negative control that does not contain a metal interacting moiety. In some embodiments the kit also includes a container to hold a system suitability solution (e.g., an equimolar mixture of the positive control and the negative control).
1 . A kit for evaluating liquid chromatography (LC) system and column inertness, the kit comprising:
a first vial comprising a positive control comprising a metal interacting moiety, wherein the positive control is selected from the group consisting of 2′(3′)-O-(4-Benzoylbenzoyl) adenosine 5′- triphosphate (Bz-ATP), ATP labeled with 6-aminohexyladenosine, ATP labeled with methylanthraniloyl, ATP labeled with trinitrophenyl, ATP labeled with fluorescein, and ATP labeled with pyrene; and
a second vial comprising a negative control comprising caffeine, the negative control not containing a metal interacting moiety, wherein both the positive control and the negative control are used as probe compounds for the detection of exposed metal in LC system and column.
2 . A kit for evaluating liquid chromatography (LC) system and column inertness, the kit comprising:
a first vial comprising a positive control comprising a metal interacting moiety, wherein the positive control is ATP or a chemical analog thereof; and
a second vial comprising a negative control comprising adenosine, the negative control not containing a metal interacting moiety, wherein both the positive control and the negative control are used as probe compounds for the detection of exposed metal in LC system and column.
3 . The kit of claim 1 , wherein the positive control consists of Bz-ATP.
4 . The kit of claim 2 , wherein the first vial and the second vial comprise equimolar amounts of the positive control and the negative control, respectively.
5 . A kit for evaluating liquid chromatography (LC) system and column inertness, the kit comprising:
a positive control comprising a metal interacting moiety, wherein the positive control is adenosine 5′-(α, β-methylene) diphosphate (AMPcP) or a chemical analog thereof;
a negative control comprising adenosine or caffeine and that does not contain a metal interacting moiety, wherein both the positive control and the negative control are used as probe compounds for the detection of exposed metal in LC system and column; and
a container to hold a system suitability solution.
6 . The kit of claim 5 , wherein the negative control consists of adenosine or substantially adenosine.
7 . The kit of claim 5 , wherein the negative control consists of caffeine or substantially caffeine.
8 . The kit of claim 5 , wherein the system suitability solution comprises an equimolar mixture of the negative control and the positive control.
9 . The kit of claim 2 , wherein the positive control comprises a chemical analog of ATP, and the chemical analog of ATP is 2′(3′)-O-(4-Benzoylbenzoyl) adenosine 5′-triphosphate (Bz-ATP).
10 . The kit of claim 2 , wherein the positive control comprises a chemical analog of ATP, and the chemical analog of ATP is etheno adenosine 5′-(α,β- methylene) diphosphate with the following structure:
11 . The kit of claim 2 , wherein the positive control comprises a chemical analog of ATP, and the chemical analog of ATP is 6-aminohexyladenosine.
12 . The kit of claim 2 , wherein the positive control comprises a chemical analog of ATP, and the chemical analog of ATP is 2-aminopurine.
13 . The kit of claim 2 , wherein the positive control comprises a chemical analog of ATP, and the chemical analog of ATP is ATP labeled with methylanthraniloyl.
14 . The kit of claim 2 , wherein the positive control comprises a chemical analog of ATP, and the chemical analog of ATP is ATP labeled with trinitrophenyl.
15 . The kit of claim 2 , wherein the positive control comprises a chemical analog of ATP, and the chemical analog of ATP is ATP labeled with fluoroscein.
16 . The kit of claim 2 , wherein the positive control comprises a chemical analog of ATP, and the chemical analog of ATP is ATP labeled with pyrene.
17 . The kit of claim 2 , wherein the negative control and the positive control have different spectral properties.
18 . A kit for evaluating liquid chromatography (LC) system and column inertness, the kit comprising:
a first vial comprising a positive control comprising a metal interacting moiety, wherein the positive control is etheno adenosine 5′-(α,β-methylene) diphosphate or ATP labeled with 2-aminopurine; and
a second vial comprising a negative control comprising caffeine, the negative control not containing a metal interacting moiety, wherein both the positive control and the negative control are used as probe compounds for the detection of exposed metal in LC system and column.