TL1A associated antibody compositions and methods of use
The disclosure herein relates to the development and production of novel antibodies and antigen binding fragments thereof that bind TL1A and that are useful in the treatment, prevention and diagnosis of a disease, disorder or inflammation including, for example, autoimmune diseases including rheumatoid arthritis, inflammatory bowel disease, atopic dermatitis, systemic lupus erythematosus, asthma, ulcerative colitis, Crohn's disease, psoriasis, primary biliary cirrhosis, primary biliary cholangitis, ankylosing spondylitis, and fibrosis including intestinal fibrosis, pulmonary fibrosis, and liver fibrosis. Some of the elements of final antibody structure being designed de novo by a computer system and its data training set without reference to a specific reference molecule.
1 . An antibody or antigen-binding fragment thereof, comprising:
(a) a variable heavy chain complementarity-determining region CDR-H1, CDR-H2 and CDR-H3, wherein:
i. CDR-H1 comprise the polypeptide sequence of SEQ ID NO: 4,
ii. CDR-H2 comprises the polypeptide sequence of SEQ ID NO: 5,
iii. CDR-H3 comprises the polypeptide sequence of SEQ ID NO: 20; and
(b) a variable light chain complementarity-determining region CDR-L1, CDR-L2 and CDR-L3, wherein
i. CDR-L1 comprises the polypeptide sequence of SEQ ID NO: 19,
ii. CDR-L2 comprises the polypeptide sequence of GAS, and
iii. CDR-L3 comprises the polypeptide sequence of SEQ ID NO: 3.
2 . An antibody or antigen-binding fragment thereof, comprising:
(a) a variable heavy chain complementarity-determining region CDR-H1, CDR-H2 and CDR-H3, wherein:
i. CDR-H1 comprise the polypeptide sequence of SEQ ID NO: 28,
ii. CDR-H2 comprises the polypeptide sequence of SEQ ID NO: 29,
iii. CDR-H3 comprises the polypeptide sequence of SEQ ID NO: 30; and
(b) a variable light chain complementarity-determining region CDR-L1, CDR-L2 and CDR-L3, wherein
i. CDR-L1 comprises the polypeptide sequence of SEQ ID NO: 27,
ii. CDR-L2 comprises the polypeptide sequence of GAS, and
iii. CDR-L3 comprises the polypeptide sequence of SEQ ID NO: 3.
3 . An antibody or antigen-binding fragment thereof, comprising:
(a) a variable heavy chain complementarity-determining region CDR-H1, CDR-H2 and CDR-H3, wherein:
i. CDR-H1 comprise the polypeptide sequence of SEQ ID NO: 4,
ii. CDR-H2 comprises the polypeptide sequence of SEQ ID NO: 5,
iii. CDR-H3 comprises the polypeptide sequence of SEQ ID NO: 20; and
(b) a variable light chain complementarity-determining region CDR-L1, CDR-L2 and CDR-L3, wherein
i. CDR-L1 comprises the polypeptide sequence of SEQ ID NO: 41,
ii. CDR-L2 comprises the polypeptide sequence of SAS, and
iii. CDR-L3 comprises the polypeptide sequence of SEQ ID NO: 3.
4 . An antibody or antigen-binding fragment thereof according to any one of claims 1-3 comprising at least one of:
(a) a variable heavy chain, wherein the variable heavy chain comprises a polypeptide sequence having at least 99% sequence identity to the amino acid sequence of SEQ ID NO: 22; and
(b) a variable light chain, wherein the variable light chain comprises a polypeptide sequence having at least 99% sequence identity to the amino acid sequence of SEQ ID NO: 21.
5 . The antibody or antigen-binding fragment thereof according to claim 1 , wherein the antibody is capable of binding human TL1A.
6 . The antibody or antigen-binding fragment thereof of claim 5 , wherein the antibody comprises an IgG, IgA, IgM, or IgE antibody.
7 . The antibody or antigen-binding fragment thereof of claim 6 , wherein the IgG comprises IgG1, IgG2, IgG3, IgG4, IgGA1, or IgGA2.
8 . The antibody or antigen-binding fragment thereof of claim 5 , wherein the antibody comprises a bispecific antibody, a multispecific antibody, a multivalent antibody, a chimeric antibody, a human antibody, humanized antibody, a monoclonal antibody, a deimmunized antibody, or a combination thereof.
9 . The antibody or antigen-binding fragment thereof of claim 8 , wherein the antibody is a human antibody.
10 . The antibody or antigen-binding fragment thereof of claim 5 , wherein the antigen-binding fragment comprises a Fab, Fab′, Fab′-SH, Fv, scFv, F (ab′) 2 , a diabody, a linear antibody, or a multi-specific antibody formed from antibody fragments.
11 . The antibody or antigen-binding fragment thereof of claim 5 , wherein the antibody or antigen-binding fragment thereof is recombinant or synthetic.
12 . The antibody or antigen-binding fragment thereof of claim 5 , wherein the antibody or antigen-binding fragment thereof further comprise an enzyme, a substrate, cofactor, a fluorescent marker, a chemiluminescent marker, a peptide tag, a magnetic particle, a drug, a toxin, or a combination thereof.
13 . The antibody or antigen-binding fragment thereof of claim 5 , wherein the antibody or antigen-binding fragment thereof inhibits inflammation.
14 . The antibody or antigen-binding fragment thereof of claim 5 , wherein the antibody or antigen-binding fragment thereof inhibits binding of TL1A to DR3 on a host cell.
15 . The antibody or antigen-binding fragment thereof of claim 5 , wherein the antibody or antigen-binding fragment is capable of binding to human TL1A or human FcRn with an affinity at least up to 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10-fold higher compared to a reference antibody.
16 . A pharmaceutical composition or a medicament that comprises:
the antibody or antigen-binding fragment thereof of claim 5 ; and
a pharmaceutically acceptable carrier, excipient, or diluent.
17 . The pharmaceutical composition or medicament of claim 16 , wherein the pharmaceutical composition or medicament is formulated for administration through subcutaneous, intravenous, intradermal, intraperitoneal, intramuscular, intracerebroventricular, intracranial, intracelial, or intracerebellar administration means.
18 . The pharmaceutical composition or medicament of claim 16 , comprising an additional therapeutic agent.
19 . The pharmaceutical composition or medicament of claim 18 , wherein the additional therapeutic agent is a nonsteroidal anti-inflammatory drug, a corticosteroid, a dietary supplement such as an antioxidant, a small molecule, a therapeutic vaccine, an immunomodulator, an anti-viral agent, acetaminophen, an anti-cancer agent, a chemotherapy, or an additional anti-TL1A antibody.
20 . A method for treating a disease, disorder or inflammation in a subject in need thereof, the method comprising administering to the subject,
(a) an antibody or antigen-binding fragment thereof according to claim 5 ; or
(b) a pharmaceutical composition or medicament comprising an antibody of (a) and a pharmaceutically acceptable carrier, excipient, or diluent.
21 . The method of claim 20 , wherein the disease, disorder, or inflammation is selected from the group consisting of: autoimmune diseases including rheumatoid arthritis, inflammatory bowel disease, atopic dermatitis, systemic lupus erythematosus, asthma, ulcerative colitis, Crohn's disease, psoriasis, primary biliary cirrhosis, primary biliary cholangitis, ankylosing spondylitis, and fibrosis including intestinal fibrosis, pulmonary fibrosis, and liver fibrosis.
22 . The method of claim 20 , wherein the antibody or antigen-binding fragment thereof binds to TL1A and/or inhibits binding of TL1A to DR3 on a host cell.
23 . The method of claim 20 , wherein the antibody or antigen binding fragment thereof is administered to the subject with an additional therapeutic agent, wherein the additional therapeutic agent is one or more of an aminosalicylate, balsalazide, olsalazine, prednisone, azathioprine, 6-Mercaptopurine methotrexate; infliximab, adalimumab, certolizumab pegol, natalizumab, a nonsteroidal anti-inflammatory drug, a corticosteroid, a dietary supplement such as an antioxidant, a small molecule, a therapeutic vaccine, an immunomodulator, an anti-viral agent, acetaminophen, an anti-cancer agent, a chemotherapy, or an additional anti-TL1A antibody.