Long-term storage of black soldier fly larvae
The present disclosure provides compositions and methods for long-term storage, shipping, and stable production of black soldier flies, capable of use in waste remediation or protein production. The invention overcomes drawbacks associated with current methods of using black soldier flies, significantly increasing the period during which black soldier fly neonates can be stored and the time in which larvae mature.
1 . A method of decoupling hatching of neonate black soldier fly larvae from production, the method comprising:
providing a container;
disposing into the container a quantity of nutrient source sufficient for the larvae to enter and remain in a stable immature phase until removal, comprising:
disposing a first layer of the nutrient source after fermentation in anaerobic conditions, the first layer being above a moisture threshold of at least 65% moisture; and,
disposing a second layer of the nutrient source without prior fermentation, the second layer being below the moisture threshold;
disposing neonate black soldier fly larvae into the container within 36 hours of eclosion such that the larvae are between the first layer and the second layer;
closing the container;
stowing the container without addition of food until removal of the larvae; and,
removing the larvae from the container and disposing the larvae in organic material such that the larvae feed on the organic material and continue development.
2 . A method of decoupling hatching of neonate black soldier fly larvae from production, the method comprising:
disposing neonate black soldier fly larvae into a container with a quantity of nutrient source sufficient for the larvae to enter and remain in a stable immature phase until removal; and,
closing the container,
wherein at least a first layer of the nutrient source below the larvae is above a moisture threshold and a second layer of material below the moisture threshold is at least partially above the larvae such that the container may be stowed without addition of food.
3 . The method of claim 2 , further comprising disposing the second layer of material into the container above the larvae.
4 . The method of claim 2 , wherein the second layer of material comprises the nutrient source.
5 . The method of claim 2 , further comprising storing the container for a period of time without addition of food.
6 . The method of claim 5 , further comprising shipping the container during the period of time the container is stored without addition of food.
7 . The method of claim 5 , wherein the period of time is at least 2 weeks.
8 . The method of claim 5 , wherein the period of time is at least 1 month.
9 . The method of claim 2 , wherein the nutrient source of the first layer comprises fermented nutrient source.
10 . The method of claim 9 , further comprising fermenting the nutrient source under anaerobic conditions prior to disposing the larvae in the first layer of the nutrient source.
11 . The method of claim 9 , wherein the second layer of material comprises unfermented nutrient source.
12 . The method of claim 9 , wherein the nutrient source comprises probiotic culture.
13 . The method of claim 2 , wherein at least the first layer and the larvae are provided such that a ratio of the larvae to the nutrient source is at least 60 larvae per gram.
14 . The method of claim 2 , wherein the larvae are provided in the container such that a ratio of a volume of the container to a volume of the larvae is 500:0.61.
15 . The method of claim 2 , wherein the first layer and the second layer are provided such that a ratio of the first layer to the second layer is at least 5:1.
16 . The method of claim 2 , further comprising removing the larvae from the container and disposing the larvae in organic material such that the larvae feed on the organic material and continue development.
17 . The method of claim 2 , wherein the neonate black soldier fly larvae are disposed in the container after eclosion and before molting.
18 . The method of claim 17 , wherein the larvae are disposed in the container no later than 36 hours after eclosion.
19 . The method of claim 2 , wherein the moisture threshold is at least about 65% moisture.
20 . The method of claim 2 , wherein the moisture threshold is about 70% moisture.
21 . The method of claim 2 , wherein the moisture threshold is no more than about 75% moisture.