IP Library › Granted Patent US 12,492,229
Granted Patent B2
US 12,492,229 · App. 17/309,959 · Granted Dec 9, 2025

Antimicrobial peptides

Inventors: Hui Li (SuZhou, CN); Dilip M. Shah (St. Louis, MO)
Assignee: Donald Danforth Plant Science Center
C07K14/415A01N37/46A01N63/50A01P3/00A61P31/10C07K7/06C07K7/08C12N15/8282A61K38/00
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Quick Facts
Patent No.
US 12,492,229
App. No.
17/309,959
Granted
Dec 9, 2025
Kind
B2
Abstract

Antimicrobial SbDef1-type peptides and proteins are disclosed along with compositions comprising the SbDef1-type peptides and proteins and transgenic or genetically edited plants or microorganisms that express the SbDef1-type peptides and proteins to inhibit growth of pathogenic microbes. Such SbDef1-type peptides and proteins, compositions, plants, and microorganisms can provide for inhibition of microbial growth.

Claims (11)

1 . A method for reducing crop damage by a plant pathogenic microbe comprising the step of contacting a plant, a plant seed, or other part of said plant with an effective amount of a composition comprising an antimicrobial peptide comprising an amino acid sequence having at least 95% sequence identity across the entire length of SEQ ID NO: 1, wherein the antimicrobial peptide carries a net positive charge at neutral pH and comprises a defensin gamma core peptide carrying a net positive charge at neutral pH, said composition further comprising an agriculturally, pharmaceutically, or veterinary acceptable carrier, diluent, or excipient.

2 . The method of claim 1 , wherein the plant pathogenic microbe is a Fusarium sp., Alternaria sp., Verticillium sp., Phytophthora sp., Colletotrichum sp., Botrytis sp., Cercospora sp., Phakopsora sp., Rhizoctonia sp., Sclerotinia sp., Pythium sp., Phoma sp., Leptosphaeria sp., Gaeumannomyces sp., or Puccinia sp.

3 . The method of claim 1 , wherein the plant pathogenic microbe is a Fusarium sp., Alternaria sp., Phytophthora sp., or Botrytis sp.

4 . The method of claim 1 , wherein the defensin gamma core peptide comprises the amino acid sequence of SEQ ID NO: 3.

5 . The method of claim 4 , wherein the defensin gamma core peptide comprises the amino acid sequence of SEQ ID NO: 8.

6 . The method of claim 1 , wherein the antimicrobial peptide comprises SEQ ID NO: 1.

7 . The method of claim 1 , wherein the antimicrobial peptide comprises SEQ ID NO: 1, 5, 6, or 7.

8 . The method of claim 1 , wherein the composition further comprises an insecticide, attractant, sterilizing agent, acaricide, nematocide, or herbicide.

9 . The method of claim 1 , wherein the composition further comprises a fungicide.

10 . The method of claim 9 , wherein the fungicide comprises a polyoxine, nikkomycine, carboxy amide, aromatic carbohydrate, carboxine, morpholine, inhibitors of sterol biosynthesis, or an organophosphorous compound.

11 . The method of claim 9 , wherein the fungicide comprises an azole, triazole, and/or echinocandin fungicide.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 10, 2021
From: LI, HUI; SHAH, DILIP M.
To: DONALD DANFORTH PLANT SCIENCE CENTER
Reel/Frame 057127/0745 →
Continuity (2)
Provisional Application 62789035 · Jan 7, 2019
Related Publication 20220081475A1 · Mar 17, 2022
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