Microorganism comprising variant LysE and method of L-amino acid production using same
Provided are a microorganism comprising variant LysE, and an L-amino acid producing method using same. The variant LysE may improve L-amino acid excretion and/or production capacity compared to a wild type.
1 . A polypeptide having L-lysine exporter activity, comprising an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 1, wherein the polypeptide comprises an amino acid residue other than asparagine at a position corresponding to the position 65 of SEQ ID NO: 1.
2 . The polypeptide of claim 1 , wherein the residue at the position corresponding to the position 65 of SEQ ID NO: 1 is substituted with glutamic acid, arginine, valine, lysine, histidine, leucine, alanine, phenylalanine, aspartic acid, isoleucine, tryptophan, proline, cysteine, tyrosine, serine, threonine, methionine, glutamine, or glycine.
3 . A polynucleotide encoding the polypeptide of claim 1 .
4 . The polynucleotide of claim 3 , represented by the nucleic acid sequence of SEQ ID NO: 4.
5 . A recombinant vector comprising the polynucleotide of claim 3 .
6 . An L-amino acid producing microorganism, comprising the polypeptide of claim 1 , a polynucleotide encoding the polypeptide, or a recombinant vector comprising the polynucleotide.
7 . The L-amino acid producing microorganism of claim 6 , wherein the polypeptide has activity of L-amino acid exporter.
8 . The L-amino acid producing microorganism of claim 6 , wherein the polypeptide or the polynucleotide is derived from a microorganism belongs to the same species of the L-amino acid producing microorganism.
9 . The L-amino acid producing microorganism of claim 6 , wherein the microorganism belongs to the genus of Corynebacterium or Escherichia.
10 . The L-amino acid producing microorganism of claim 6 , wherein the microorganism is Corynebacterium glutamicum or Escherichia coli.
11 . The L-amino acid producing microorganism of claim 6 , having an improved exporting ability or production potential of L-amino acid, compared to an unmodified microorganism.
12 . The L-amino acid producing microorganism of claim 7 , wherein the L-amino acid is L-lysine, L-arginine, or a combination thereof.
13 . A method of producing an L-amino acid, the method comprising:
culturing the L-amino acid producing microorganism of claim 7 in a medium.
14 . The method of producing an L-amino acid of claim 13 , further comprising, after the step of culturing,
recovering the L-amino acid from the cultured microorganism, the medium, or both of them.
15 . The method of producing an L-amino acid of claim 13 , wherein the L-amino acid is L-lysine, L-arginine, or a combination thereof.