Electronically-controlled digital ferrofluidic device and method for scalable and addressable bioanalytical operations
An electronically-controlled digital ferrofluidic device is disclosed which employs a network of individually addressable coils in conjunction with one or more movable permanent magnets, where each moveable permanent magnet delivers the designated fluid manipulation-based tasks. The underlying mechanism facilitating fluidic operations is realized by addressable electromagnetic actuation of miniaturized mobile magnets that exert localized magnetic body forces on droplets filled with magnetic nanoparticles. The reconfigurable, contactless, and non-interfering magnetic-field operation properties of the underlying actuation mechanism allow for the integration of passive and active components to implement advanced and diverse operations with high efficiency (e.g., droplet sorting, dispensing, generation, merging, mixing, filtering, and analysis).
1 . A ferrofluidic fluid handling device comprising:
a first substrate defining a navigation floor and having a plurality of individually addressable coils formed therein or thereon across x and y dimensions;
a second substrate comprising one or more channels, chambers, regions, zones, or wells formed therein and disposed to above the first substrate and separated by a gap region having a height;
one or more permanent magnets interposed in the gap region formed between the first substrate and the second substrate, the one or more permanent magnets having respective heights less than the height of the gap region; and
a power source and control circuitry electrically connected to the individually addressable coils;
wherein the one or more permanent magnets are moveable laterally over the navigation floor in the x and y directions in response to actuation of the individually addressable coils.
2 . The device of claim 1 , wherein the first substrate comprises a printed circuit board (PCB) having a matrix of individually addressable coils formed therein or thereon.
3 . The device of claim 2 , wherein each individually addressable coil comprises a multi-layered spiral disposed in the PCB.
4 . The device of claim 1 , wherein the second substrate comprises an upper chamber fluidically coupled to a lower chamber by one or more vias or holes.
5 . The device of claim 1 , further comprising one or more volumes of ferrofluid contained in the one or more channels, chambers, regions, zones, or wells, the ferrofluid comprising magnetic particles contained therein.
6 . The device of claim 1 , wherein the one or more channels, chambers, regions, zones, or wells comprises a corrugated wall.
7 . The device of claim 6 , wherein the corrugated wall comprises a plurality of uniformly dimensioned openings formed in a wall of the one or more channels, chambers, regions, zones, or wells.
8 . The device of claim 1 , wherein the one or more permanent magnets are dimensioned in a lateral direction to cover or at least partially overlap one or more of the individually addressable coils.
9 . The device of claim 1 , wherein the one or more permanent magnets comprise rare earth magnets, a metallic material, or composite magnetic material.
10 . The device of claim 1 , wherein the one or more channels, chambers, regions, zones, or wells are enclosed.
11 . The device of claim 1 , wherein the one or more channels, chambers, regions, zones, or wells are open.
12 . The device of claim 1 , wherein the one or more channels, chambers, regions, zones, or wells are fluidically coupled to a plurality of separate chambers or wells.
13 . The device of claim 1 , further comprising an optical interrogation device configured to image the one or more channels, chambers, regions, zones, or wells of the second substrate.
14 . The device of claim 1 , wherein the one or more channels, chambers, regions, zones, or wells comprises an oil therein.
15 . The device of claim 1 , wherein the control circuitry comprises a microcontroller unit (MCU).
16 . The device of claim 15 , further comprising a computing device in communication with the MCU and comprising software executed by one or more processors that contain a program for the sequence and timing of actuation of the individually addressable coils.