Granted Patent B2

US 12,533,412 · App. 18/570,072 · Granted Jan 27, 2026

Inventors: Lianhui Wang (Jiangsu, CN); Lihui Yuwen (Jiangsu, CN); Weijun Xiu (Jiangsu, CN)

Assignee: Nanjing University of Posts and Telecommunications

A61K41/0028A61K9/1271A61K9/1277A61K9/5123A61K9/5192A61K31/4164A61P31/04

Abstract

Claims (23)

1 . Ultrasound-responsive liposome nanoparticles, comprising:

a lipid material, comprising dimyristoylphosphatidylcholine, 1,2-dioleoyl-3-trimethylammonium-propane and distearoylphosphatidylethanolamine-polyethylene glycol;

a sonosensitizer;

an antibiotic; and

perfluoropentane.

2 . The ultrasound-responsive liposome nanoparticles according to claim 1 , wherein the perfluoropentane has a volumetric proportion of 1%-2% in the ultrasound-responsive liposome nanoparticles.

3 . The ultrasound-responsive liposome nanoparticles according to claim 1 , wherein the sonosensitizer is at a concentration of 0.5-1 mg/mL in the ultrasound-responsive liposome nanoparticles.

4 . The ultrasound-responsive liposome nanoparticles according to claim 3 , wherein the sonosensitizer is chlorin.

5 . The ultrasound-responsive liposome nanoparticles according to claim 1 , wherein the antibiotic is at a concentration of 0.5-1 mg/mL in the ultrasound-responsive liposome nanoparticles.

6 . The ultrasound-responsive liposome nanoparticles according to claim 5 , wherein the antibiotic is metronidazole.

7 . The ultrasound-responsive liposome nanoparticles according to claim 1 , wherein the dimyristoylphosphatidylcholine, the 1,2-dioleoyl-3-trimethylammonium-propane and the distearoylphosphatidylethanolamine-polyethylene glycol are in a mass ratio of 5:1.5:1.

8 . A method of preparing the ultrasound-responsive liposome nanoparticles according to claim 1 , comprising the following steps:

Step 1: dissolving the dimyristoylphosphatidylcholine, the 1,2-dioleoyl-3-trimethylammonium-propane, the distearoylphosphatidylethanolamine-polyethylene glycol, the sonosensitizer and the antibiotic in chloroform, and performing rotary evaporation at 50° C. for 5-10 min to form a liposome film;

Step 2: after resuspending the liposome film with ionized water, treating hydrated liposome with an ultrasonic probe in an ice-water bath, wherein during treatment process, the perfluoropentane is slowly added dropwise, and can be gradually wrapped by the hydrated liposome in a process of ultrasonic hydration and self-assembly, to form a liposome nanoparticle dispersion; and

Step 3: putting the liposome nanoparticle dispersion after reaction in a dialysis bag, and dialyzing in a phosphate buffered saline solution to obtain drug-loaded liposome nanoparticles.

9 . The method of preparing the ultrasound-responsive liposome nanoparticles according to claim 8 , wherein working conditions of the ultrasonic probe in Step 2 are: working for 5 s, an interval of 2 s, a power of 40%, and ultrasonic time of 5-10 min.

10 . The method of preparing the ultrasound-responsive liposome nanoparticles according to claim 8 , wherein the dialysis bag used in Step 3 has a molecular weight cutoff of 10 kDa, and dialysis time is 24-48 h.

11 . The method of preparing the ultrasound-responsive liposome nanoparticles according to claim 8 , wherein the phosphate buffered saline solution used in dialysis in Step 3 has a pH of 7.4 and a concentration of 10 mM.

12 . The method of preparing the ultrasound-responsive liposome nanoparticles according to claim 8 , wherein the drug-loaded liposome nanoparticles in Step 3 are the ultrasound-responsive liposome nanoparticles and have a size of 150-250 nm.

13 . A method of using the ultrasound-responsive liposome nanoparticles of claim 1 in a treatment of bacterial biofilm infections, comprising:

preparing a bacterial biofilm;

adding a dispersion comprising the ultrasound-responsive liposome nanoparticles of claim 1 into the bacterial biofilm; and then

applying an ultrasound treatment to the bacterial biofilm having the dispersion.

Assignments (1)

Priority Claims (1)

Continuity (1)

References Cited (17)