IP Library › Granted Patent US 12,533,675
Granted Patent B2
US 12,533,675 · App. 18/597,358 · Granted Jan 27, 2026

Methods for conducting multiplexed assays

Inventors: Eli N. Glezer (Del Mar, CA); Sudeep Kumar (Gaithersburg, MD); Pankaj Oberoi (Rockville, MD); George Sigal (Rockville, MD); Michael Tsionsky (Derwood, MD)
Assignee: MESO SCALE TECHNOLOGIES, LLC.
B01L3/50853C12Q1/6804C12Q1/6816C12Q1/6876G01N33/54306G01N33/54353G01N33/6845B01L2300/021B01L2300/043B01L2300/0609B01L2300/0829G01N2458/10
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Quick Facts
Patent No.
US 12,533,675
App. No.
18/597,358
Granted
Jan 27, 2026
Kind
B2
Abstract

The invention relates to methods for conducting solid-phase binding assays. One example is an assay method having improved analyte specificity where specificity is limited by the presence of non-specific binding interactions.

Claims (112)

1 . A pair of oligonucleotides wherein the pair of oligonucleotides comprises complementary sequences, wherein the pair is selected from:

Pair

Sequence

 1

acatcggtagtt (SEQ ID NO: 1)

aactaccgatgt (SEQ ID NO: 2)

 3

agaagaagatcc (SEQ ID NO: 5)

ggatcttcttct (SEQ ID NO: 6)

 6

atcattaccacc (SEQ ID NO: 11)

ggtggtaatgat (SEQ ID NO: 12)

 7

attaacgggagc (SEQ ID NO: 13)

gctcccgttaat (SEQ ID NO: 14)

 8

cagaggtcttaa (SEQ ID NO: 15)

ttaagacctctg (SEQ ID NO: 16)

 9

caggtgtccatt (SEQ ID NO: 17)

aatggacacctg (SEQ ID NO: 18)

11

cctacgatatac (SEQ ID NO: 21)

gtatatcgtagg (SEQ ID NO: 22)

12

cgaatgtagagt (SEQ ID NO: 23)

actctacattcg (SEQ ID NO: 24)

13

cggtttgagata (SEQ ID NO: 25)

tatctcaaaccg (SEQ ID NO: 26)

16

gacataaagcga (SEQ ID NO: 31)

tcgctttatgtc (SEQ ID NO: 32)

17

gccatagtctct (SEQ ID NO: 33)

agagactatggc (SEQ ID NO: 34)

18

gctaattcacca (SEQ ID NO: 35)

tggtgaattagc (SEQ ID NO: 36)

20

gttgattctgtc (SEQ ID NO: 39)

gacagaatcaac (SEQ ID NO: 40)

23

ttccacttaggg (SEQ ID NO: 45)

ccctaagtggaa (SEQ ID NO: 46)

25

tttcccttgcta (SEQ ID NO: 49)

tagcaagggaaa (SEQ ID NO: 50)

and wherein each of the pair of oligonucleotides is modified with a linking complex formed by a reaction between: (i) a thiol group and a maleimide or iodoacetamide; (ii) an aldehyde and a hydrazide; or (iii) an alkyne and an azide.

2 . A kit comprising, in separate vials, at least 4 oligonucleotides, each comprising a different sequence selected from:

Pair

Sequence

 1

acatcggtagtt (SEQ ID NO: 1)

aactaccgatgt (SEQ ID NO: 2)

 3

agaagaagatcc (SEQ ID NO: 5)

ggatcttcttct (SEQ ID NO: 6)

 6

atcattaccacc (SEQ ID NO: 11)

ggtggtaatgat (SEQ ID NO: 12)

 7

attaacgggagc (SEQ ID NO: 13)

gctcccgttaat (SEQ ID NO: 14)

 8

cagaggtcttaa (SEQ ID NO: 15)

ttaagacctctg (SEQ ID NO: 16)

 9

caggtgtccatt (SEQ ID NO: 17)

aatggacacctg (SEQ ID NO: 18)

11

cctacgatatac (SEQ ID NO: 21)

gtatatcgtagg (SEQ ID NO: 22)

12

cgaatgtagagt (SEQ ID NO: 23)

actctacattcg (SEQ ID NO: 24)

13

cggtttgagata (SEQ ID NO: 25)

tatctcaaaccg (SEQ ID NO: 26)

16

gacataaagcga (SEQ ID NO: 31)

tcgctttatgtc (SEQ ID NO: 32)

17

gccatagtctct (SEQ ID NO: 33)

agagactatggc (SEQ ID NO: 34)

18

gctaattcacca (SEQ ID NO: 35)

tggtgaattagc (SEQ ID NO: 36)

20

gttgattctgtc (SEQ ID NO: 39)

gacagaatcaac (SEQ ID NO: 40)

23

ttccacttaggg (SEQ ID NO: 45)

ccctaagtggaa (SEQ ID NO: 46)

25

tttcccttgcta (SEQ ID NO: 49)

tagcaagggaaa (SEQ ID NO: 50)

wherein each of the oligonucleotides in the kit is modified with a linking complex formed by a reaction between: (i) a thiol group and a maleimide or iodoacetamide; (ii) an aldehyde and a hydrazide; or (iii) an alkyne and an azide.

3 . The kit of claim 2 , wherein the kit comprises at least 7 oligonucleotides.

4 . The kit of claim 2 , wherein the kit comprises at least 10 oligonucleotides.

5 . The kit of claim 2 , wherein the kit comprises at least 16 oligonucleotides.

6 . The kit of claim 2 , wherein the kit comprises at least 25 oligonucleotides.

7 . The kit of claim 2 , wherein each of the oligonucleotides in the kit is coupled to an antibody.

8 . The kit of claim 2 , wherein each of the oligonucleotides in the kit is coupled to a different antibody.

9 . The kit of claim 2 , wherein each different sequence is not a complement of an additional different sequence of the at least 4 oligonucleotides.

10 . The kit of claim 2 , wherein the kit further comprises assay reagents that are stored in a dry state.

11 . The kit of claim 2 , wherein the kit further comprises a desiccant material.

12 . The kit of claim 2 , wherein the kit comprises a surface selected from the group consisting of a multi-well plate and a plurality of particles.

13 . The kit of claim 2 , wherein the oligonucleotides are surface bound oligonucleotides and the kit further comprises corresponding oligonucleotide complements.

14 . The kit of claim 2 , further comprising a labeling kit for attaching a detectable label to an assay component.

15 . The kit of claim 14 , wherein the assay component is a detection reagent.

16 . The pair of oligonucleotides of claim 1 , wherein each oligonucleotide of the pair of oligonucleotides is modified on the 3′ end.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Mar 7, 2024
From: GLEZER, ELI N.; KUMAR, SUDEEP; OBEROI, PANKAJ; SIGAL, GEORGE; TSIONSKY, MICHAEL
To: MESO SCALE TECHNOLOGIES, LLC.
Reel/Frame 066679/0910 →
Continuity (6)
Continuation 17215962 · Mar 29, 2021
Division 16227081 · Dec 20, 2018
Continuation 15784399 · Oct 16, 2017
Continuation 14847761 · Sep 8, 2015
Provisional Application 62047097 · Sep 8, 2014
Related Publication 20240375109A1 · Nov 14, 2024
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