IP Library › Granted Patent US 12,545,888
Granted Patent B2
US 12,545,888 · App. 18/674,014 · Granted Feb 10, 2026

Nanobubbles for anaerobic processes

Inventors: John William Crisman (Eudora, KS); Andrea Renee Turriciano White (Sparks, NV)
Assignee: Moleaer, Inc.
C12N1/34C02F3/26C02F3/28
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Quick Facts
Patent No.
US 12,545,888
App. No.
18/674,014
Granted
Feb 10, 2026
Kind
B2
Abstract

Provided herein are methods and systems for treating a stream comprising material (e.g., biomass such as plant biomass, animal biomass, agricultural biomass, wastewater sludge, or a combination thereof) with nanobubbles. One such method includes generating nanobubbles in wastewater sludge of a wastewater treatment system to produce nanobubble-containing sludge, the wastewater sludge having a higher solids content than an input stream to the wastewater treatment system, and processing the nanobubble-containing sludge in an anaerobic reactor of the wastewater treatment system.

Claims (19)

1 . A method comprising:

generating nanobubbles directly in a stream of a system to produce a nanobubble-containing stream using at least one nanobubble generator, wherein the stream has a solid concentration of at least 1,200 mg/L;

introducing the nanobubble-containing stream to an anaerobic reactor of the system; and

anaerobically processing the nanobubble-containing stream in an anaerobic reactor of the system.

2 . The method of claim 1 , wherein a dissolved oxygen content of the stream before the nanobubble generation is the same as a dissolved oxygen content of the nanobubble-containing stream.

3 . The method of claim 1 , wherein the stream has a solid concentration that is between 1,200 mg/L and 90,000 mg/L.

4 . The method of claim 1 , wherein the stream has a dry solid content of at least 0.12% by weight.

5 . The method of claim 1 , wherein the stream has a dry solid content that is between 0.12% and 4.5% by weight.

6 . The method of claim 1 , wherein a gas in the nanobubbles is selected from air, argon, hydrogen, biogas, methane, carbon dioxide, nitrogen, oxygen, and ozone.

7 . The method of claim 1 , wherein the stream comprises biomass.

8 . The method of claim 7 , wherein the biomass comprises plant biomass, animal biomass, agricultural biomass, wastewater sludge, or a combination thereof.

9 . The method of claim 1 , wherein the system is a wastewater treatment system, an energy production system, a fertilizer production system, or an anaerobic fermentation system.

10 . The method of claim 1 , wherein the system is a wastewater treatment system, and the stream comprises wastewater sludge.

11 . A method comprising:

generating nanobubbles directly in a stream of a system to produce a nanobubble-containing stream using at least one nanobubble generator, wherein the stream has a chemical oxygen demand of at least 2,000 mg/L and/or the stream has a biological oxygen demand of at least 1,000 mg/L;

introducing the nanobubble-containing stream to an anaerobic reactor of the system; and

anaerobically processing the nanobubble-containing stream in an anaerobic reactor of the system.

12 . The method of claim 11 , wherein the stream has a chemical oxygen demand that is between 2,000 mg/L and 5,000,000 mg/L.

13 . The method of claim 11 , wherein the stream has a biological oxygen demand that is between 1,000 mg/L and 2,500,000 mg/L.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 18, 2024
From: CRISMAN, JOHN WILLIAM; TURRICIANO WHITE, ANDREA RENEE
To: MOLEAER, INC.
Reel/Frame 067753/0196 →
Continuity (2)
Provisional Application 63469302 · May 26, 2023
Related Publication 20240392236A1 · Nov 28, 2024
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