Method and system for evaluation of drug concentration in blood, plasma or serum
A method and a system for evaluating a drug concentration in body fluid samples, wherein the body fluid samples are blood, plasma or serum, the method including the steps of: preparing at least one body fluid sample including a solved target drug by removing unsolved components from the body fluid sample; performing absorption spectrophotometric analyzes on the at least one body fluid sample; and evaluating a drug concentration in the at least one body fluid sample based on the spectrophotometric analyzes.
1 . A method for evaluating a pharmaceutical concentration in body fluid samples taken from a patient during treatment of the patient with the pharmaceutical, wherein the pharmaceutical comprises an antibiotic or cytostatic, and wherein said body fluid samples are blood, plasma or serum, said method comprising the steps of:
preparing at least one body fluid sample comprising the pharmaceutical in a solved state by
diluting the body fluid sample by adding a volume of clean water or saline which is at least 50% of the body fluid sample volume; and
removing unsolved components from the body fluid sample;
performing absorption spectrophotometric analysis on the at least one body fluid sample using UV-VIS light with wavelengths between 200-800 nm;
evaluating a concentration of the pharmaceutical in the at least one body fluid sample based on the spectrophotometric analysis, wherein the step of evaluating the concentration in the body fluid sample comprises comparing the spectrophotometric analysis with pre-stored spectrophotometric spectra for the pharmaceutical at different concentrations and with a patient reference spectra,
wherein the patient reference spectra is a spectrophotometric spectra for at least one body fluid sample from the patient taken before a start of the treatment or in the beginning of the treatment of the patient with the pharmaceutical.
2 . The method according to claim 1 , wherein a pharmaceutical concentration is evaluated in at least two body fluid samples taken at different times during treatment of a patient with said pharmaceutical and wherein the method further comprises the step of:
determining a change in pharmaceutical concentration between the at least two body fluid samples by comparing the spectrophotometric analysis for the different body fluid samples.
3 . The method according to claim 1 , further comprising the step of
monitoring pharmaceutical concentration changes over time in a body fluid from a patient during treatment of said patient with said pharmaceutical by evaluating a pharmaceutical concentration in a number of body fluid samples taken at different times during treatment of a patient with said pharmaceutical.
4 . The method according to claim 1 , wherein the step of preparing the at least one body fluid sample comprises removing lipids and blood cells.
5 . The method according to claim 1 , wherein the step of preparing at least one body fluid sample comprises:
centrifuging the body fluid sample; and
collecting a fraction from the centrifuged body fluid sample for further use in the spectrophotometric analysis.
6 . The method according to claim 1 , wherein the step of preparing the at least one body fluid sample comprises filtering the body fluid sample before performing the spectrophotometric analysis.
7 . The method according to claim 1 , wherein the step of preparing at least one body fluid sample comprises:
diluting the at least one body fluid sample;
centrifuging the body fluid sample;
collecting a middle fraction from the centrifuged body fluid sample;
filtering said middle fraction through a bacteria filter ( 5 ) for further use in the spectrophotometric analysis.
8 . The method according to claim 1 , wherein said method is performed bed side, during treatment of a patient with the pharmaceutical.
9 . A method for administering of a pharmaceutical to a patient comprising:
adapting a pharmaceutical dose to be administered to the patient in accordance with an evaluation of a pharmaceutical concentration in body fluid samples taken from the patient during the administration of the pharmaceutical according to claim 1 .
10 . The method according to claim 9 , wherein said adaptation of the pharmaceutical dose and said evaluation of a pharmaceutical concentration in a body fluid sample are done repeatedly during treatment of the patient with the pharmaceutical.
11 . The method of claim 1 , wherein the antibiotic is cefotaxim and the cytostatic is carboplatin.