Receptacle transport system for an analytical system
Providing a fluid to an instrument comprises supporting a sample receptacle on a first carrier, transporting the first carrier on a conveyor adjacent a plurality of modules including at least one instrument, stopping the first carrier at the instrument, transporting the sample receptacle to a pick-up position of the instrument, transporting the sample receptacle from the pick-up position to a pipetting station within the instrument, aspirating fluid within the sample receptacle and transferring the aspirated fluid to a reaction receptacle, after aspirating fluid from the sample receptacle, transporting the sample receptacle to the pick-up position, transporting the sample receptacle from the pick-up position to a second carrier the conveyor, in the instrument, performing an assay to determine the presence or absence of an analyte in the aspirated fluid, and transporting the second carrier on the conveyor to one or more of the plurality of modules other than the analytical instrument.
1 . A method for providing a fluid to an instrument located adjacent a conveyor for transporting receptacles between a plurality of modules, the method comprising the steps of:
(a) supporting a sample receptacle in an upright orientation on a first carrier;
(b) transporting the first carrier on a conveyor extending adjacent to each of a plurality of modules, at least one of the modules being an analytical instrument;
(c) stopping the first carrier at a position adjacent the analytical instrument;
(d) after step (c), and while the first carrier remains on the conveyor, removing the sample receptacle from the first carrier and transporting the sample receptacle to a pick-up position of the analytical instrument;
(e) transporting the sample receptacle from the pick-up position to a pipetting station located within the analytical instrument;
(f) at the pipetting station, aspirating a fluid contained within the sample receptacle and transferring the aspirated fluid to a reaction receptacle supported by the analytical instrument;
(g) after aspirating the fluid from the sample receptacle, transporting the sample receptacle from the pipetting station to the pick-up position;
(h) removing the sample receptacle from the pick-up position and transporting the sample receptacle to a second carrier located on the conveyor adjacent the analytical instrument, the second carrier supporting the sample receptacle in an upright orientation;
(i) in the analytical instrument, performing an assay with the aspirated fluid, thereby determining the presence or absence of an analyte in the aspirated fluid; and
(j) transporting the second carrier supporting the sample receptacle on the conveyor to one or more of the plurality of modules other than the analytical instrument.
2 . The method of claim 1 , wherein the first carrier is a puck having a cylindrically shaped base and a pocket formed in a top surface of the base for seating the sample receptacle.
3 . The method of claim 2 , wherein the puck has a plurality of upwardly extending fingers for supporting the sample receptacle in the upright orientation.
4 . The method of claim 1 , wherein the conveyor comprises a stationary track for supporting the first carrier during step (b).
5 . The method of claim 4 , wherein the first carrier is propelled on the track by a magnetic attraction between the first carrier and the conveyor.
6 . The method of claim 1 , wherein the analytical instrument is an instrument for performing nucleic acid-based amplification reactions.
7 . The method of claim 1 , wherein step (c) is performed with a stop element operationally associated with the conveyor, and wherein the stop element is actuated from an open position allowing passage of the first carrier on the conveyor to a closed position during step (c), the stop element immobilizing the first carrier in the closed position.
8 . The method of claim 1 , wherein the sample receptacle is removed from the first carrier and transported to the pick-up position with a gripper apparatus.
9 . The method of claim 1 further comprising the step of determining whether the height and orientation of the sample receptacle is acceptable.
10 . The method of claim 1 , wherein a receptacle holder supported by a carriage receives the sample receptacle at the pick-up position in step (d).
11 . The method of claim 10 , wherein the pick-up position is located outside of a housing of the analytical instrument.
12 . The method of claim 10 , wherein the carriage transports the sample receptacle from the pick-up position and to the pipetting station in step (d).
13 . The method of claim 12 further comprising the step of securing the sample receptacle in the carriage as the sample receptacle is transported from the pick-up position to the pipetting station, thereby impeding vertical movement of the sample receptacle.
14 . The method of claim 1 , wherein the first carrier and the second carrier are the same carrier.
15 . The method of claim 1 , wherein the assay comprises exposing the sample to reagents and conditions for performing a nucleic acid-based amplification reaction.