IP Library › Granted Patent US 12,551,586
Granted Patent B2
US 12,551,586 · App. 17/850,408 · Granted Feb 17, 2026

Methods, systems, and apparatus for sterilization, disinfection, and purification

Inventor: Lawrence Revel Johnson (Leawood, KS)
A61L2/0076A61L2/0047A61L2/0088A61L2202/22
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Quick Facts
Patent No.
US 12,551,586
App. No.
17/850,408
Granted
Feb 17, 2026
Kind
B2
Abstract

In one illustrative example, a method for use in sterilization involves carrying a liquid or a flow of liquid comprising water; converting the liquid or the flow thereof into mist or steam; adding riboflavin in soluble form as a photosensitizer to the liquid or the flow thereof, converting the liquid or the flow thereof into mist or steam that carries the riboflavin; discharging the mist or the steam that carries the riboflavin into a chamber, a container, or a room; and emitting, on the mist or the steam that carries the riboflavin, a riboflavin-activating light sufficient to activate the riboflavin to enhance a cross-linking of genetic material including the amino acids of proteins of cells or pathogens or extracellular genetic material in the chamber, the container, or the room. Additional processing steps may be employed for disrupting barriers, for increased access of the riboflavin and light to the genetic material.

Claims (45)

1 . A method comprising:

carrying a liquid or a flow of liquid comprising water;

adding riboflavin in soluble form as a photosensitizer to the liquid or the flow thereof;

heating the liquid or the flow thereof into steam that carries the riboflavin and releasing it into air; and

emitting, on the steam that carries the riboflavin in the air, a riboflavin-activating light sufficient to activate the riboflavin to enhance a cross-linking of genetic material of cells or pathogens or extracellular genetic material in the air, for sterilization of the genetic material of the cells or the pathogens or the extracellular genetic material in the air, for thereby producing sterilized air to release or circulate in a chamber, a container, or a room,

wherein heating the liquid or the flow thereof into the steam that carries the riboflavin is sufficient to disrupt barriers of the cells or the pathogens in the air for increased access of the riboflavin and the riboflavin-activating light to the genetic material of the cells or the pathogens.

2 . The method of claim 1 , wherein the riboflavin-activating light comprises ultraviolet light C (UV-C) from one or more UV-C light-emitting diodes (LEDs).

3 . The method of claim 1 , further comprising:

repeating steps of the method in an ongoing process for producing the sterilized air to recirculate through the chamber, the container, or the room.

4 . The method of claim 1 , further comprising:

prior to releasing or circulating the sterilized air in the chamber, the container, or the room, generating ultrasonic waves through the steam that carries the riboflavin in the air, sufficient to disrupt the barriers of the cells or the pathogens for further increased access of the riboflavin and the riboflavin-activating light to the genetic material of the cells or the pathogens for increased sterilization.

5 . The method of claim 1 , further comprising:

prior to releasing or circulating the sterilized air in the chamber, the container, or the room, exerting and maintaining increased pressure on the steam that carries the riboflavin in the air, sufficient to disrupt the barriers of the cells or the pathogens for further increased access of the riboflavin and the riboflavin-activating light to the genetic material of the cells or the pathogens for increased sterilization.

6 . The method of claim 1 , wherein the increased access of the riboflavin and the riboflavin-activating light to the genetic material of the cells or the pathogens is for increased sterilization.

7 . A method comprising:

carrying a liquid or a flow of liquid comprising water;

adding riboflavin in soluble form as a photosensitizer to the liquid or the flow thereof;

heating the liquid or the flow thereof into steam that carries the riboflavin;

discharging the steam that carries the riboflavin into a chamber, a container, or a room; and

emitting, on the steam that carries the riboflavin, a riboflavin-activating light sufficient to activate the riboflavin to enhance a cross-linking of genetic material of cells or pathogens or extracellular genetic material in the chamber, the container, or the room, for sterilization of the genetic material of the cells or the pathogens or the extracellular genetic material in the chamber, the container, or the room,

wherein heating the liquid or the flow thereof into the steam that carries the riboflavin is sufficient to disrupt barriers of the cells or the pathogens or surface films in the chamber, the container, or the room, for increased access of the riboflavin and the riboflavin-activating light to the genetic material of the cells or the pathogens.

8 . The method of claim 7 , wherein the riboflavin-activating light comprises ultraviolet light C (UV-C) from one or more UV-C light-emitting diodes (LEDs).

9 . The method of claim 7 , wherein the emitting of the riboflavin-activating light on the steam that carries the riboflavin causes one or more surfaces or objects in the chamber, the container, or the room to be disinfected.

10 . The method of claim 7 , further comprising:

generating ultrasonic waves in the chamber, the container, or the room, sufficient to disrupt the barriers of the cells or the pathogens or the surface films in the chamber, the container, or the room, for further increased access of the riboflavin and the riboflavin-activating light to the genetic material of the cells or the pathogens for increased sterilization.

11 . The method of claim 7 , further comprising:

exerting and maintaining an increased pressure on the steam that carries the riboflavin, sufficient to disrupt the barriers of the cells or the pathogens or the surface films in the chamber, the container, or the room, for further increased access of the riboflavin and the riboflavin-activating light to the genetic material of the cells or the pathogens for increased sterilization.

12 . The method of claim 7 , wherein the increased access of the riboflavin and the riboflavin-activating light to the genetic material of the cells or the pathogens is for increased sterilization.

13 . The method of claim 7 , wherein the adding of the riboflavin in the liquid or the flow thereof comprises:

regularly or continuously injecting the riboflavin in soluble form into regularly or continuously replenished liquid or flow thereof.

14 . A method comprising:

carrying a liquid or a flow of liquid comprising water;

adding riboflavin in soluble form as a photosensitizer in the liquid or the flow thereof;

converting the liquid or the flow thereof into mist or steam that carries the riboflavin;

discharging the mist or the steam that carries the riboflavin into a chamber, a container, or a room;

emitting, on the mist or the steam that carries the riboflavin, a riboflavin-activating light sufficient to activate the riboflavin to enhance a cross-linking of genetic material of cells or pathogens or extracellular genetic material in the chamber, the container, or the room, for sterilization of the genetic material of the cells or the pathogens or the extracellular genetic material in the chamber, the container, or the room; and

exerting and maintaining increased pressure on the mist or the steam that carries the riboflavin sufficient to disrupt barriers of the cells or the pathogens or surface films in the chamber, the container, or the room, for increased access of the riboflavin and the riboflavin-activating light to the genetic material of the cells or the pathogens.

15 . The method of claim 14 , wherein the riboflavin-activating light comprises ultraviolet light C (UV-C) from one or more UV-C light-emitting diodes (LEDs).

16 . The method of claim 14 , further comprising:

generating ultrasonic waves in the mist or the steam that carries the riboflavin, sufficient to disrupt the barriers of the cells or the pathogens in the liquid, for increased access of the riboflavin and the riboflavin-activating light to the genetic material of the cells or the pathogens for increased sterilization.

17 . The method of claim 14 , wherein the increased access of the riboflavin and the riboflavin-activating light to the genetic material of the cells or the pathogens is for increased sterilization.

18 . The method of claim 14 , wherein the converting of the liquid or the flow thereof into the mist or the steam that carries the riboflavin further comprises:

heating the liquid or the flow thereof into the steam that carries the riboflavin.

19 . The method of claim 1 , wherein the liquid is water.

20 . The method of claim 7 , wherein the liquid is water.

Continuity (1)
Related Publication 20230414798A1 · Dec 28, 2023
References Cited (38)
US 7094378B1 · Goodrich, Jr. et al. · 2006 [cited by applicant]
US 10940260B2 · Igarashi · 2021 [cited by applicant]
US 11147876B2 · Sowemimo-Coker · 2021 [cited by applicant]
US 20030214874A1 · Hlavinka · 2003 [cited by examiner]
US 20030215784A1 · Dumont · 2003 [cited by examiner]
US 20100057060A1 · Herekar · 2010 [cited by applicant]
US 20110210268A1 · Dornseifer · 2011 [cited by applicant]
US 20120118150A1 · Brizes et al. · 2012 [cited by applicant]
US 20150307368A1 · Yanke · 2015 [cited by applicant]
US 20160088853A1 · Tikekar et al. · 2016 [cited by applicant]
US 20160206768A1 · Mullins · 2016 [cited by applicant]
US 20180055960A1 · Reiber et al. · 2018 [cited by applicant]
US 20180338905A1 · Shapiro et al. · 2018 [cited by applicant]
US 20200030469A1 · Neister et al. · 2020 [cited by applicant]
US 20200030790A1 · Dodd · 2020 [cited by examiner]
US 20210346531A1 · Kim · 2021 [cited by examiner]
US 20220143243A1 · Chen · 2022 [cited by examiner]
CN 204521168U · 2015 [cited by applicant]
CN 104758119B · 2017 [cited by applicant]
CN 109908376B · 2021 [cited by applicant]
EP 1469891B1 · 2003 [cited by applicant]
KR 20150032044A · 2015 [cited by applicant]
WO 03066109A1 · 2003 [cited by applicant]
WO 2021072927A1 · 2021 [cited by applicant]
Damen et al., “Effect of Ultra-High Temperature Steam Injection Processing and Aseptic Storage on Labile Water-Soluble Vitamins in Milk,” Journal of Diary Science, vol. 72, No. 3, 1989. [cited by applicant]
Alotaibi et al., “Solar Disinfection of Water for Inactivation of Enteric Viruses and its Enhancement by Riboflavin,” Food and Environmental Virology, Article No. 70, May 27, 2011. [cited by applicant]
Beggs et al., “Upper-room ultraviolet air disinfection might help to reduce COVID-19 transmission in buildings,” PeerJ doi: 10.7717/peerj.10196, Jun. 16, 2020. [cited by applicant]
Hadi et al., “Control Measures for SARS-CoV-2: A Review on Light-Based Inactivation of Single-Stranded RNA Viruses,” Pathogens 2020; Sep. 8, 2020. [cited by applicant]
Martins et al., “Antimicrobial Efficacy of Riboflavin/UVA Combination (365 nm) In Vitro for Bacterial and Fungal Isolates: A Potential New Treatment for Infectious Keratitis,” Investigative Ophthalmology & Visual Scienc… [cited by applicant]
Shen et al., “Effect of Ultraviolet Light Irradiation Combined with Riboflavin on Different Bacterial Pathogens from Ocular Surface Infection,” Journal of Biophysics, vol. 2017, Article ID 3057329. [cited by applicant]
Xu et al., “Vitamin K5 is an efficient photosensitizer for ultraviolet A light inactivation of bacteria,” FEMS Microbiology Letters, 365, Jan. 15, 2018. [cited by applicant]
Gambro Press Release, “Gambro awarded US patent for its unique process of inactivation of pathogens in blood components,” Jul. 27, 2001. [cited by applicant]
Ragan et al., “Pathogen Reduction of SARS-CoV-2 Virus in Plasma and Whole Blood Using Riboflavin and UV Light,” Department of Biomedical Sciences, Infectious Disease Research Center, Translational Medicine Institute, Ma… [cited by applicant]
Sadraeian et al., “Study of Viral Photoinactivation by UV-C Light and Phtosensitizer Using a Pseudotyped Model,” Pharmaceutics 2022, Mar. 21, 2022. [cited by applicant]
Sangsom et al., “Design and Development of Innovative Steam Injection for High-Temperature Short-Time Liquid Foods,” Processes 2022, Jan. 14, 2022. [cited by applicant]
ShineLong Technology Corp., Ltd., “An A-to-Z Guide on What There is to Know About UVC LED Lights,” https://www.shinelongled.com/uvc-led-light/, downloaded Nov. 21, 2021, 23 pages. [cited by applicant]
Waveform Lighting LLC, “An Introduction to UV-C LED Lighting for Germicidal, Sterilization and Disinfection Applications,” https://www.waveformlighting.com/uv-c-led/uv-c-led-lighting-for-germicidal-sterilization-and-dis… [cited by applicant]
International Light Technologies Inc., “UV-C LEDs for UVGI / Disinfection,” https://www.intl-lighttech.com/applications/uvc-leds, downloaded Nov. 21, 2021, 6 pages. [cited by applicant]