IP Library › Granted Patent US 12,553,069
Granted Patent B2
US 12,553,069 · App. 17/760,462 · Granted Feb 17, 2026

Isopropylmalate synthase polypeptide variant and a method for producing L-leucine using the same

Inventors: Hayun Lee (Seoul, KR); Ju Eun Kim (Seoul, KR); Jihyun Shim (Seoul, KR); Ji Hye Lee (Seoul, KR); Sung Gun Lee (Seoul, KR)
Assignee: CJ CHEILJEDANG CORPORATION
C12P13/06C12N9/1025C12N15/77C12Y203/03013C12N2800/101
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Quick Facts
Patent No.
US 12,553,069
App. No.
17/760,462
Granted
Feb 17, 2026
Kind
B2
Abstract

The present application relates to: a novel mutant polypeptide having isopropylmalate synthase activity; and a method for producing L-leucine by using same. L-leucine can be produced at high yield by using the mutant polypeptide according to an embodiment.

Claims (17)

1 . A mutated polypeptide with isopropylmalate synthase activity, comprising an amino acid sequence at least 90% identical to amino acid residues 36-616 of SEQ ID NO: 1 that is a wild-type sequence, wherein the mutated polypeptide has an amino acid residue other than proline at a position corresponding to position 247 in the amino acid sequence of SEQ ID NO: 1 and wherein the amino acid residues 36-616 are set forth in SEQ ID NO: 16.

2 . The mutated polypeptide of claim 1 , wherein the position corresponding to position 247 in the amino acid sequence of SEQ ID NO: 1 is substituted with cysteine.

3 . The mutated polypeptide of claim 1 , wherein the mutated polypeptide comprises the amino acid sequence of SEQ ID NO: 3.

4 . The mutated polypeptide of claim 1 , wherein the mutated polypeptide has an amino acid residue other than arginine at a position corresponding to position 558 in the amino acid sequence of SEQ ID NO: 1.

5 . The mutated polypeptide of claim 4 , wherein the mutated polypeptide comprises the amino acid sequence of SEQ ID NO: 5.

6 . The mutated polypeptide of claim 1 , wherein the mutated polypeptide has an amino acid residue other than a glycine at a position corresponding to position 561 in the amino acid sequence of SEQ ID NO: 1.

7 . The mutated polypeptide of claim 1 , wherein the mutated polypeptide has

an amino acid residue other than an arginine at a position corresponding to position 558 in the amino acid sequence of SEQ ID NO: 1, and

an amino acid residue other than a glycine at a position corresponding to position 561 in the amino acid sequence of SEQ ID NO: 1.

8 . The mutated polypeptide of claim 1 , wherein the mutated polypeptide has histidine at a position corresponding to position 558 in the amino acid sequence of SEQ ID NO: 1.

9 . The mutated polypeptide of claim 1 , wherein the mutated polypeptide has aspartic acid at a position corresponding to position 561 in the amino acid sequence of SEQ ID NO: 1.

10 . The mutated polypeptide of claim 1 , wherein the mutated polypeptide has

histidine at a position corresponding to position 558 in the amino acid sequence of SEQ ID NO: 1; and

aspartic acid at a position corresponding to position 561 in the amino acid sequence of SEQ ID NO: 1.

11 . The mutated polypeptide of claim 1 , comprising an amino acid sequence at least 98% identical to the amino acid sequence of SEQ ID NO: 16.

12 . The mutated polypeptide of claim 1 , comprising an amino acid sequence at least 95% identical to the amino acid sequence of SEQ ID NO:1.

13 . The mutated polypeptide of claim 1 , comprising an amino acid sequence at least 99% identical to the amino acid sequence of SEQ ID NO: 1.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 10, 2022
From: LEE, HAYUN; KIM, JU EUN; SHIM, JIHYUN; LEE, JI HYE; LEE, SUNG GUN
To: CJ CHEILJEDANG CORPORATION
Reel/Frame 060767/0686 →
Priority Claims (1)
KR 10-2020-0060578 · May 20, 2020 · national
Continuity (1)
Related Publication 20230088135A1 · Mar 23, 2023
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