Granted Patent B2

US 12,559,799 · App. 17/586,143 · Granted Feb 24, 2026

Inventors: Christopher Raymond (Kirkland, WA); Lee Lim (Kirkland, WA); Jennifer Hernandez (Kirkland, WA)

Assignee: Resolution Bioscience, Inc.

C12Q1/6886C12Q1/68C12Q1/6806C12Q1/6809C12Q1/686C12Q1/6869G16B20/00G16B20/10G16B20/20G16B20/40G16B25/10G16B30/00G16B30/10C12Q1/6851C12Q2525/191C12Q2545/114C12Q2563/159C12Q2563/179C12Q2600/156C40B40/08G01N2570/00G01N2800/7028

Abstract

Claims (46)

1 . A kit comprising a set of adaptors,

wherein each adaptor of the set of adaptors comprises a sample tag region selected from a pool of unique sample tag regions, wherein the pool is selected from a plurality of pools, and wherein the selected pool is unique to a test sample;

wherein the test sample comprises a plurality of DNA fragments;

wherein each adaptor of the set of adaptors is a DNA polynucleotide that comprises (i) an amplification region, (ii) a sample tag region; and (iii) an anchor region; and

wherein each adaptor of the set of adaptors further comprises a unique molecule identifier multiplier (UMI multiplier) that increases the number of unique sequences for identifying the plurality of DNA fragments.

2 . The kit of claim 1 , wherein the amplification region comprises a polynucleotide sequence capable of serving as a primer recognition site for PCR amplification.

3 . The kit of claim 1 , wherein the sample tag region identifies the test sample.

4 . The kit of claim 1 , wherein the anchor region comprises a polynucleotide sequence that is capable of attaching to a DNA fragment.

5 . The kit of claim 1 , wherein each adaptor of the set of adaptors is configured to attach to a DNA fragment of the plurality of DNA fragments to generate a DNA library comprising at least two unique sample tag regions, wherein each of the DNA library fragments comprises a DNA fragment attached to an adaptor.

6 . The kit of claim 1 , wherein the test sample is a tissue biopsy.

7 . The kit of claim 1 , wherein the DNA fragments are cell-free DNA (cfDNA) or cellular DNA.

8 . The kit of claim 1 , wherein the DNA fragments are genomic DNA.

9 . The kit of claim 7 , wherein the DNA fragments are isolated from the test sample; and wherein the test sample is a biological sample selected from the group consisting of: amniotic fluid, blood, plasma, serum, semen, lymphatic fluid, cerebral spinal fluid, ocular fluid, urine, saliva, stool, mucous, and sweat.

10 . The kit of claim 1 , wherein the amplification region of each adaptor of the set of adaptors is identical to the amplification region of every other adaptor of the set of adaptors.

11 . The kit of claim 1 , wherein the sample tag region identifies the DNA fragment attached thereto.

12 . The kit of claim 1 , wherein the UMI multiplier is adjacent to or contained within the sample tag region.

13 . The kit of claim 1 , wherein the pool of sample tag regions comprises between 2 and 1,000 unique sample tag region sequences.

14 . The kit of claim 1 , further comprising one or more capture probe modules.

15 . The kit of claim 14 , wherein each capture probe module comprises a tail sequence and a capture probe sequence capable of hybridizing to a target sequence in the test sample.

16 . A DNA library, wherein the DNA library comprises a plurality of DNA library fragments, wherein each of the DNA library fragments comprises an adaptor and a DNA fragment,

wherein the adaptor is a DNA polynucleotide comprising (i) an amplification region, (ii) a sample tag region, and (iii) an anchor region;

wherein the amplification region comprises a polynucleotide sequence capable of serving as a primer recognition site for PCR amplification;

wherein the sample tag region identifies the test sample;

wherein the anchor region comprises a polynucleotide sequence that is capable of attaching to a DNA fragment; and

wherein each adaptor of the set of adaptors further comprises a unique molecule identifier multiplier (UMI multiplier) that increases the number of unique sequences for identifying the plurality of DNA library fragments.

17 . A method for genetic analysis of a DNA target region comprising:

(a) generating a DNA library using the kit of claim 5 , wherein the DNA library comprises cfDNA;

(b) contacting the DNA library with a plurality of capture probe modules that specifically bind to a DNA target region, thereby forming complexes between the capture probe modules and DNA library fragments comprising the DNA target region; and

(c) performing a quantitative genetic analysis of the DNA library fragments comprising the DNA target region;

thereby performing genetic analysis of the DNA target region.

18 . A method of predicting, diagnosing, or monitoring a genetic disease in a subject comprising:

(a) obtaining a test sample from a subject;

(b) isolating DNA from the test sample;

(c) generating a DNA library comprising a plurality of DNA library fragments using the kit of claim 5 , wherein each of the DNA library fragments comprises a DNA fragment from the test sample and an adaptor;

(d) contacting the DNA library with a plurality of capture probe modules that specifically bind to a DNA target region, thereby forming complexes between the capture probe modules and DNA library fragments comprising the DNA target region; and

(e) performing a quantitative genetic analysis of one or more target genetic loci associated with the genetic disease in the DNA library, wherein the identification or detection of one or more genetic lesions in the one or more target genetic loci is prognostic for, diagnostic of, or monitors the progression of the genetic disease.

19 . A set of adaptors,

wherein each adaptor of the set of adaptors comprises a sample tag region selected from a pool of unique sample tag regions, wherein the pool is selected from a plurality of pools, and wherein the selected pool is unique to a test sample;

wherein each adaptor in said set of adapters is a DNA polynucleotide that comprises: an amplification region, a sample tag region, and an anchor region;

wherein the amplification region comprises a polynucleotide sequence capable of serving as a primer recognition site for PCR amplification;

wherein the sample tag region identifies the test sample;

wherein the anchor region comprises a polynucleotide sequence that is capable of attaching to a DNA fragment; and

wherein each adaptor of the set of adaptors further comprises a unique molecule identifier multiplier (UMI multiplier) that increases the number of unique sequences for identifying the plurality of DNA fragments.

20 . The set of adaptors of claim 19 , wherein the UMI multiplier is adjacent to or contained within the sample tag region.

21 . The set of adaptors of claim 19 , wherein the sample tag region identifies the DNA fragment attached thereto.

22 . The set of adaptors of claim 19 , wherein the pool of sample tag regions comprises between 2 and 1,000 unique sample tag region sequences.

Assignments (2)

Continuity (4)

References Cited (337)