Compositions and methods for weed control
The present disclosure relates to, inter alia, methods and compositions for weed control, for example, a method of selectively controlling weeds at a locus comprising crop plants and weeds by applying to the locus a weed controlling amount of a pesticide composition comprising a SDPS-inhibiting herbicide, wherein the crop plants are modified such that they comprise a SDPS which provides the crop plant with tolerance against the SPDS-inhibiting herbicide. Compositions also include, inter alia, recombinant polynucleotides suitable for use in the methods.
1 . A recombinant polynucleotide comprising:
a nucleic acid sequence that encodes a modified Solanesyl Diphosphate Synthase (SDPS) comprising SEQ ID NO: 5 with the phenylalanine at amino acid 247 replaced with leucine, operably linked to a plant operable promoter, wherein expression of the modified SDPS in a plant confers the plant with increased tolerance to a Solanesyl Diphosphate Synthase-inhibiting herbicide compared to a control plant not expressing the modified SDPS and expressing a native SDPS.
2 . A plant cell comprising a recombinant polynucleotide stably incorporated into the genome of the plant cell, the recombinant polynucleotide comprising a nucleic acid sequence encoding a modified Solanesyl Diphosphate Synthase (SDPS) comprising SEQ ID NO: 5 with the phenylalanine at amino acid 247 replaced with leucine, operably linked to a plant operable promoter, wherein expression of the modified SDPS in said plant cell confers the plant cell with increased tolerance to a Solanesyl Diphosphate Synthase-inhibiting herbicide compared to a control plant cell expressing a native SDPS and not expressing the modified SDPS.
3 . The recombinant polynucleotide of claim 1 , wherein the plant operable promoter is heterologous to the nucleic acid encoding the SDPS.
4 . A vector comprising the recombinant polynucleotide of claim 1 .
5 . A plant comprising a recombinant polynucleotide stably incorporated into the genome of the plant, the recombinant polynucleotide comprising a nucleic acid sequence encoding a modified Solanesyl Diphosphate Synthase (SDPS) comprising SEQ ID NO: 5 with the phenylalanine at amino acid 247 replaced with leucine, operably linked to a plant operable promoter, wherein expression of the modified SDPS in said plant confers the plant with increased tolerance to a Solanesyl Diphosphate Synthase-inhibiting herbicide compared to a control plant cell not expressing the modified SDPS and expressing a native SDPS.
6 . The plant of claim 5 , wherein the plant comprises a seed and said recombinant polynucleotide is stably incorporated into said seed.
7 . The plant of claim 5 , wherein said plant is a monocot.
8 . The plant of claim 7 , wherein said monocot is corn or wheat.
9 . The plant of claim 5 , wherein said plant is a dicot.
10 . The plant of claim 9 , wherein said dicot is soybean, sunflower or cotton.
11 . The plant of claim 6 , wherein said plant is a monocot.
12 . The plant of claim 11 , wherein said monocot is corn or wheat.
13 . The plant of claim 6 , wherein said plant is a dicot.
14 . The plant of claim 13 , wherein said dicot is soybean, sunflower or cotton.