Recombinant humanized collagen type III alpha-1, and expression vector and use thereof
A recombinant humanized collagen type III alpha-1 (rhCol III α1), and an expression vector and use thereof are provided, relating to the technical field of bioengineering. A sequence on positions 154 to 1,221 of a human type III collagen α chain is ligated to an optimized vector pcDNA3.1, a resulting optimized expression vector is transfected into a human embryonic kidney cell Expi293F, and then rhCol III α1 is secreted to form a fusion protein with a soluble green fluorescent protein (GFP). The expressed rhCol III α1 that forms the fusion protein with the GFP shows an activity of promoting the migration of BALB/c 3T3 cells, thus exhibiting a great application potential in the field of skin care.
1 . A recombinant humanized collagen type III alpha-1 (rhCol III α1) fusion protein consisting of SEQ ID NO: 3 for use in a skin care product, the rhCol III α1 fusion protein being encoded by a fused nucleotide sequence, and wherein the fused nucleotide sequence comprises sequentially a gene sequence for encoding a secretion signal peptide, a gene sequence for encoding a 8× histidine tag, a gene sequence for encoding a green fluorescent protein (GFP), a DNA sequence for encoding a digestion sequence of a tobacco etch virus (TEV) protease, and a gene sequence for encoding rhCol III α1;
wherein the rhCol III α1 fusion protein has the amino acid sequence consisting of SEQ ID NO: 3; and
wherein the gene sequence for encoding rhCol III α1 has the sequence consisting of SEQ ID NO: 1.
2 . A product for skin repair, comprising the rhCol III α1 fusion protein according to claim 1 in the product for skin repair.