Methods and systems for point-of-care coagulation assays by optical detection
An assay system includes an excitation light source to output excitation light having a first wavelength; an optical reference positioned to absorb the excitation light from the excitation light source and to output an optical signal based on the excitation light, where the optical signal has a second wavelength that is different from the first wavelength; a reaction chamber containing a reactant and an inlet to receive a test sample to produce a fluid that is based on the test sample and the reactant, where the reaction chamber is positioned to receive the optical signal from the optical reference; and an optical receiver positioned to detect an amount of the optical signal that passed through the reaction chamber containing the fluid.
1 . An assay system comprising:
an excitation light source to output excitation light having a first wavelength;
an optical reference positioned to absorb the excitation light from the excitation light source and to output an optical signal based on the excitation light, the optical signal having a second wavelength that is different from the first wavelength;
a reaction chamber comprising a reactant and an inlet to receive a test sample to produce a fluid that is based on the test sample and the reactant, the reaction chamber being positioned to receive the optical signal from the optical reference;
an optical receiver positioned to detect an amount of the optical signal that passed through the reaction chamber containing the fluid;
wherein the optical reference is positioned between the excitation light source and the reaction chamber, and wherein the reaction chamber is positioned between the optical reference and the optical receiver; and
a test instrument to detect an analyte or coagulation associated with the test sample based on the amount of the optical signal that passed through the reaction chamber containing the fluid.
2 . The assay system of claim 1 , wherein the reaction chamber holds the test sample in the absence of a colorimetric reagent.
3 . The assay system of claim 1 , wherein the first wavelength ranges from 20 nm (nanometers) to 5000 nm.
4 . The assay system of claim 1 , wherein the optical reference is selected from the group consisting of fluorescence doped glass, stained glass, dyed glass, materials showing Raman effect, fluorescent polymer film, and fluorescent polymer sheet.
5 . The assay system of claim 1 , wherein the reaction chamber comprises a lumen, a planar first wall, and a planar second wall that is opposite and parallel to the planar first wall.
6 . The assay system of claim 5 , wherein the planar first wall and the planar second wall are each optically transparent to light in a wavelength range of about 20 nm (nanometers) to about 5000 nm.
7 . The assay system of claim 6 , wherein the first wavelength is in a range of about 20 nm to about 2000 nm.
8 . The assay system of claim 5 , wherein each of the planar first and second walls of the reaction chamber comprises a luminal surface, and wherein a luminal surface is coated with the reactant.
9 . The assay system of claim 1 , wherein the optical receiver and the excitation light source are integrated.
10 . The assay system of claim 1 , wherein the optical receiver comprises a light detector.
11 . The assay system of claim 1 , wherein the reaction chamber further comprises a reaction fluid outlet port.
12 . The assay system of claim 1 , wherein the inlet is V-shaped.
13 . The assay system of claim 1 , wherein the optical receiver comprises a light detector for detecting the amount of the optical signal that passed through the reaction chamber containing the fluid.
14 . The assay system of claim 1 , wherein the optical signal is a fluorescence signal.
15 . The assay system of claim 1 , wherein the reaction chamber comprises more than one reactant.
16 . The assay system of claim 1 , wherein the reactant comprises a reagent.
17 . The assay system of claim 16 , wherein the reagent is a dry reagent.