US 4516590A
· Teng
· 1985
[cited by applicant]
US 4528993A
· Sensabaugh, Jr. et al.
· 1985
[cited by applicant]
US 4660577A
· Sensabaugh et al.
· 1987
[cited by applicant]
US 4732856A
· Federoff
· 1988
[cited by applicant]
US 4762785A
· Comai
· 1988
[cited by applicant]
US 4848373A
· Lenkey
· 1989
[cited by applicant]
US 4945050A
· Sanford et al.
· 1990
[cited by applicant]
US 4987907A
· Townend
· 1991
[cited by applicant]
US 5004863A
· Umbeck
· 1991
[cited by applicant]
US 5013658A
· Dooner et al.
· 1991
[cited by applicant]
US 5104310A
· Saltin
· 1992
[cited by applicant]
US 5141131A
· Miller, Jr. et al.
· 1992
[cited by applicant]
US 5149645A
· Hoekema et al.
· 1992
[cited by applicant]
US 5159135A
· Umbeck
· 1992
[cited by applicant]
US 5177010A
· Goldman et al.
· 1993
[cited by applicant]
US 5231019A
· Paszkowski et al.
· 1993
[cited by applicant]
US 5316931A
· Donson et al.
· 1994
[cited by applicant]
US 5372149A
· Roth et al.
· 1994
[cited by applicant]
US 5463174A
· Moloney et al.
· 1995
[cited by applicant]
US 5464763A
· Schilperoort et al.
· 1995
[cited by applicant]
US 5469976A
· Burchell
· 1995
[cited by applicant]
US 5491081A
· Webb
· 1996
[cited by applicant]
US 5563055A
· Townsend et al.
· 1996
[cited by applicant]
US 5589367A
· Donson et al.
· 1996
[cited by applicant]
US 5659026A
· Baszczynski et al.
· 1997
[cited by applicant]
US 5689035A
· Webb
· 1997
[cited by applicant]
US 5789156A
· Bujard et al.
· 1998
[cited by applicant]
US 5814618A
· Bujard et al.
· 1998
[cited by applicant]
US 5866785A
· Donson et al.
· 1999
[cited by applicant]
US 5879918A
· Tomes et al.
· 1999
[cited by applicant]
US 5886244A
· Tomes et al.
· 1999
[cited by applicant]
US 5889190A
· Donson et al.
· 1999
[cited by applicant]
US 5889191A
· Turpen
· 1999
[cited by applicant]
US 5932782A
· Bidney
· 1999
[cited by applicant]
US 5981840A
· Zhao et al.
· 1999
[cited by applicant]
US 6072050A
· Bowen et al.
· 2000
[cited by applicant]
US 7700834B2
· Xu et al.
· 2010
[cited by applicant]
US 8124851B2
· Dewey et al.
· 2012
[cited by applicant]
US 8319011B2
· Xu et al.
· 2012
[cited by applicant]
US 9187759B2
· Dewey et al.
· 2015
[cited by applicant]
US 9228194B2
· Dewey et al.
· 2016
[cited by applicant]
US 9228195B2
· Dewey et al.
· 2016
[cited by applicant]
US 9247706B2
· Dewey et al.
· 2016
[cited by applicant]
US 20040118422A1
· Lundin et al.
· 2004
[cited by applicant]
US 20120199148A1
· Xu et al.
· 2012
[cited by applicant]
CN 101611146A
· 2009
[cited by applicant]
CN 102858983A
· 2013
[cited by applicant]
CN 103993023A
· 2014
[cited by applicant]
CN 105960460A
· 2016
[cited by applicant]
JP 2020524508A
· 2020
[cited by applicant]
WO WO2004041006
· 2004
[cited by applicant]
WO WO2011027315
· 2011
[cited by applicant]
WO WO2016210303A1
· 2016
[cited by applicant]
WO WO2018237107A1
· 2018
[cited by applicant]
Wijnker, Erik, and Hans de Jong. “Managing meiotic recombination in plant breeding.” Trends in plant science 13.12 (2008): 640-646. (Year: 2008).
[cited by examiner]
Accession PI 112309 of the US Nicotiana Germplasm Collection, U.S. National Plant Germplasm, 2 pages, (Sep. 1935), available online: https://npgsweb.ars-grin.gov/gringlobal/accessiondetail?id=1129722.
[cited by applicant]
Agrawal et al., “RNA Interference: Biology, Mechanism, and Applications,” Microbiology and Molecular Biology Reviews, 67(4):657-685 (Dec. 2003), available online: DOI: 10.1128/MMBR.67.4.657-685.2003.
[cited by applicant]
Bowman et al., “Revised North Carolina Grade Index for Flue-Cured Tobacco,”
[cited by applicant]
Burner et al., “Analyses of diverse low alkaloid tobacco germplasm identify naturally occurring nucleotide variability contributing to reduced leaf nicotine accumulation,”
[cited by applicant]
Centers for Disease Control and Prevention's Protocol for Analysis of Nicotine, Total Moisture and pH in Smokeless Tobacco Products, as published in the Federal Register vol. 64, No. 55, pp. 13897-13912 (Mar. 1999)(and …
[cited by applicant]
Cermak et al., “Efficient Design and Assembly of Custom TALEN and Other TAL Effector-Based Constructs for DNA Targeting,”
[cited by applicant]
Chaplin, “Interrelationships Between the Pale-Yellow Character and Other Traits in Flue-Cured Tobacco,”
[cited by applicant]
Chaplin et al., “Agronomic, chemical, and smoke characteristics of flue-cured tobacco lines with different levels of total alkaloids.”
[cited by applicant]
Chaplin et al., “Association Between Percent Total Alkaloids and Other Traits in Flue-Cured Tobacco,”
[cited by applicant]
Chapters 4B and 4C of Tobacco, Production, Chemistry and Technology, Davis & Nielsen Editions, Blackwell Publishing, Oxford, pp. 70-103. (1999).
[cited by applicant]
Chinese Search Report issued Chinese Patent Application No. 201980014896X, dated Apr. 8, 2022, with English translation (7 pages).
[cited by applicant]
Christensen et al., “Sequence Analysis and Transcriptional Regulation by Heat Shock of Polyubiquitin Transcripts from Maize,”
[cited by applicant]
Christensen et al., “Maize Polyubiquitin Genes: Structure, Thermal Perturbation of Expression and Transcript Splicing, and Promoter Activity Following Transfer to Protoplasts by Electroporation,”
[cited by applicant]
Christou et al., “Stable Transformation of Soybean Callus by DNA-Coated Gold Particles,”
[cited by applicant]
Collins et al., “Determination of Nicotine Alkaloids in Tobacco Using the Autoanalyzer,”
[cited by applicant]
“CORESTA Recommended Method No. 7: Determination of Nicotine in the Mainstream Smoke of Cigarettes by Gas Chromatographic Analysis,” pp. 1-5, (1987) (updated Aug. 1991) (Paris, France).
[cited by applicant]
“CORESTA Recommended Method No. 62: Determination of Nicotine in Tobacco and Tobacco Products by Gas Chromatographic Analysis,” Coresta Cooperation Centre for Scientific Research Relative to Tobacco (Feb. 2005) (Version…
[cited by applicant]
Crossway et al., “Micromanipulation Techniques in Plant Biotechnology,”
[cited by applicant]
Database UniProt Accession No. A0A1S4AQ55 Subname: Full=ethylene-responsive transcription factor 13-like {EC0:00003131RefSeq:XP_016478716.1} XP002789317, retrieved from EBI Database (Apr. 2017).
[cited by applicant]
Davis et al., “A Combined Automated Procedure for the Determination of Reducing Sugars and Nicotine Alkaloids in Tobacco Products Using a New Reducing Sugar Method,”
[cited by applicant]
D'Halluin et al., “Transgenic Maize Plants by Tissue Electroporation,”
[cited by applicant]
De Wet et al., “Exogenous gene Transfer in Maize (
[cited by applicant]
Doyle et al., “TAL Effector-Nucleotide Targeter (TALE-NT) 2.0: tools for TAL Effector Design and Target Prediction,”
[cited by applicant]
“Draft for Diplomatic Conference for the Revision of the International Convention for the Protection of New Varieties of Plants,” Mar. 4-19, 1991 (Geneva, Switzerland).
[cited by applicant]
Estruch et al., “Transgenic plants: An Emerging Approach to Pest Control,”
[cited by applicant]
Federal Register vol. 64, No. 55 Mar. 23, 1999, as amended in vol. 74, No. 4, Jan. 7, 2009.
[cited by applicant]
Fedoroff et al., “Cloning of the bronze Locus in Maize by a Simple and Generalizable Procedure Using the Transposable Controlling Element Activator (Ac),”
[cited by applicant]
Finer et al., “Transformation of Soybean via Particle Bombardment of Embryogenic Suspension Culture Tissue,”
[cited by applicant]
Gaj et al., “ZFN, TALEN and CRISPR/Cas-based Methods for Genome Engineering,”
[cited by applicant]
Gatz et al., “Regulation of a Modified CaM V35S Promoter by the Tn10- encoded Tet Repressor in Transgenic Tobacco,”
[cited by applicant]
Hibi et al., “Putrescine N-Methyltransferase in Cultured Roots of Hyoscyamus albus1: n-Butylamine as a Potent Inhibitor of the Transferase both in Vitro and in Vivo,”
[cited by applicant]
Hildering et al., “Chimeric Structure of the Tomato Plant After Seed Treatment With EMS and X-Rays,” The Use of Induced Mutations in Plant Breeding (Supplement to Radiation Botany), vol. 5, Pergamon Press Ltd., pp. 317-…
[cited by applicant]
Hiratsu, et al. “Dominant repression of target genes by chimeric repressors that include the EAR motif, a repression domain, in
[cited by applicant]
Hoekema et al., “A Binary Plant Vector Strategy Based on Separation of vir- and T-Region of the
[cited by applicant]
International Search Report and Written Opinion issued in PCTUS2019/013363 on May 15, 2019 (20 pages).
[cited by applicant]
International Search Report and Written Opinion issued in PCT/US2019/013345, dated May 15, 2019 (17 pages).
[cited by applicant]
Kaeppler et al., “Silicon Carbide Fiber-Mediated DNA Delivery into Plant Cells,”
[cited by applicant]
Kaeppler et al., “Silicon Carbide Fiber-Mediated Stable Transformation of Plant Cells,”
[cited by applicant]
Kajikawa et al., “Genomic Insights into the Evolution of the Nicotine Biosynthesis Pathway in Tobacco
[cited by applicant]
Kosambi, “The Estimation of Map Distance from Recombinant Values,”
[cited by applicant]
Last et al., “pEmu: an improved promoter for gene expression in cereal cells,”
[cited by applicant]
Legg et al., “Registration of LA Burley 21 Tobacco Germplasm1 Registration No. (GP 8),”
[cited by applicant]
Legg et al., “Inheritance of Per Cent Total Alkaloids in
[cited by applicant]
McCabe et al., “Stable Transformation of Soybean (Glycine max) by Particle Acceleration,”
[cited by applicant]
McCallum et al., “Targeted Screening for Induced Mutations,”
[cited by applicant]
McNellis et al., “Glucocorticoid-Inducible Expression of a Bacterial Avirulence Gene in Transgenic
[cited by applicant]
Miller et al., “A Grade Index for Type 22 and 23 Fire-cured Tobacco,”
[cited by applicant]
Miller “Memorandum: Proposed Burley Tobacco Grade Index,” Legacy Tobacco Document Library, The University of Tennessee Agricultural Experiment Station (Bates Document #523267826-523267833) (Jul. 1988) (Knoxville, USA).
[cited by applicant]
Morita et al., “Vacuolar Transport of Nicotine is Mediated by a Multidrug and Toxic Compound Extrusion (MATE) Transporter in Nicotiana Tabacum,”
[cited by applicant]
NCBI Genbank XM_016623230.1, “PREDICTED: Nicotiana tabacum ethylene responsive transcription factor 13-like (LOC 107800084), mRNA”, published on May 3, 2016.
[cited by applicant]
Odell et al., “Identification of DNA Sequences Required for Activity of the Cauliflower Mosaic Virus 35S Promoter,”
[cited by applicant]
Official Standard Grades for Burley Tobacco (U.S. Type 31 and Foreign Type 93), effective Nov. 5, 1990 (55 F.R. 40645).
[cited by applicant]
Official Standard Grades for Flue-Cured Tobacco (U.S. Types 11, 12, 13, 14 and Foreign Type 92), effective Mar. 27, 1989 (54 F.R. 7925).
[cited by applicant]
Official Standard Grades for Pennsylvania Seedleaf Tobacco (U.S. Type 41), effective Jan. 8, 1965 (29 F.R. 16854).
[cited by applicant]
Official Standard Grades for Ohio Cigar-Leaf Tobacco (U.S. Types 42, 43, and 44), effective Dec. 8, 1963 (28 F.R. 11719 and 28 F.R. 11926).
[cited by applicant]
Official Standard Grades for Wisconsin Cigar-Binder Tobacco (U.S. Types 54 and 55), effective Nov. 20, 1969 (34 F.R. 17061).
[cited by applicant]
Official Standard Grades for Georgia and Florida Shade-Grown Cigar-Wrapper Tobacco (U.S. Type 62), Effective Apr. 1971.
[cited by applicant]
Okuzaki et al., “Improvement of the Plastid Transformation Protocol by Modifying Tissue Treatment at Pre-and Post-Bombardment in Tobacco,”
[cited by applicant]
Paszkowski et al., “Direct Gene Transfer to Plants,”
[cited by applicant]
Poehlman et al., “Breeding Field Crops,”
[cited by applicant]
Porta et al., “Use of Viral Replicons for the Expression of Genes in Plants,”
[cited by applicant]
Qin et al. “NIC1 Cloning and Gene Editing Generates Low-Nicotine Tobacco Plants,”
[cited by applicant]
Richmond, “Chemical Topping Burley Tobacco,”
[cited by applicant]
Riggs et al., “Stable Transformation of Tobacco by Electroporation: Evidence for Plasmid Concatenation,”
[cited by applicant]
Rushton et al., TOBFAC: the Database of Tobacco Transcription Factors,
[cited by applicant]
Sanford et al., “Optimizing the Biolistic Process for Different Biological Applications,”
[cited by applicant]
Schena et al., “A Steroid-Inducible Gene Expression System for Plant Cells,”
[cited by applicant]
Sears et al., “NtERF32: a non-NIC2 locus AP2/ERF transcription factor required in jasmonate-inducible nicotine biosynthesis in tobacco,”
[cited by applicant]
Shillito et al., “Direct Gene Transfer to Protoplasts of Dicotyledonous and Monocotyledonous Plants by a Number of Methods, Including Electroporation,”
[cited by applicant]
Shoji et al., “Clustered Transcription Factor Genes Regulate Nicotine Biosynthesis in Tobacco,”
[cited by applicant]
Shoji et al., “Natural and induced variations in transcriptional regulator genes result in low-nicotine phenotypes in tobacco,”
[cited by applicant]
Shoji et al., “Tobacco MYC2 Regulates Jasmonate-Inducible Nicotine Biosynthesis Genes Directly and by Way of the NIC2-Locus ERF Genes,”
[cited by applicant]
Shoji et al., “Smoking out the masters: transcriptional regulators for nicotine biosynthesis in tobacco,”
[cited by applicant]
Singh et al., “Cytological Characterization of Transgenic Soybean,”
[cited by applicant]
Sisson et al., “Alkaloid Composition of the USDA Tobacco (
[cited by applicant]
Tomes et al., “Direct DNA Transfer into Intact Plant Cells Via Microprojectile Bombardment,”
[cited by applicant]
Tso “Seed to Smoke,” Chapter 1 in Davis and Nielsen (ed.), Tobacco: Production, Chemistry and Technology, Blackwell Science Publishing, pp. 1-31 with cover page (1999) (Oxford, UK).
[cited by applicant]
Tobacco Production, Chemistry and Technology, Edited by L. Davis and M. Nielsen, Blackwell Science, 1999.
[cited by applicant]
Velten et al., “Isolation of a Dual Plant Promoter Fragment from the Ti Plasmid of
[cited by applicant]
Verkerk, “Chimerism of the Tomato Plant After Seed Irradiation with Fast Neutrons,”
[cited by applicant]
Weissing et al., “Foreign Genes in Plants: Transfer, Structure, Expression, and Applications,”
[cited by applicant]
Wernsman et al., “Tobacco,” Chapter Seventeen: Principles of Cultivar Development,
[cited by applicant]