IP Library › Granted Patent US 12,584,103
Granted Patent B2
US 12,584,103 · App. 18/654,976 · Granted Mar 24, 2026

Hypothermic 3D bioprinting of living tissues supported by perfusable vasculature

Inventors: Jordan Miller (Houston, TX); Anderson Ta (Houston, TX); Bagrat Grigoryan (Spring, TX)
Assignee: WILLIAM MARSH RICE UNIVERSITY
C12N5/0062B29C64/129B29C64/393B33Y10/00B33Y50/02B33Y70/00C12M3/00C12M21/08C12M25/14C12N5/0068C12N5/0641B29K2071/02C12N2513/00C12N2533/30C12N2537/00
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Quick Facts
Patent No.
US 12,584,103
App. No.
18/654,976
Granted
Mar 24, 2026
Kind
B2
Abstract

The present disclosure provides compositions and methods for producing hydrogel matrix constructs. Methods of using hydrogel matrix constructs for tissue repair and regeneration and for the oxygenation of red blood cells are also disclosed.

Claims (10)

1 . A prepolymerization solution, comprising:

poly(ethylene glycol) having a molecular weight of greater than 2,000 Daltons and comprising at least two acrylate groups per molecule of poly(ethylene glycol);

a photo-initiator; and

a protein with a peak light absorption overlapping that of the photo-initiator.

2 . The prepolymerization solution of claim 1 , wherein the prepolymerization solution excludes a light-absorbing additive material.

3 . The prepolymerization solution of claim 1 , wherein the prepolymerization solution further comprises a biocompatible, light-absorbing additive material, wherein the biocompatible, light-absorbing additive material has an additional capability to cause printing, by controlling light penetration, a structure with horizontal and vertical channels.

4 . The prepolymerization solution of claim 1 , further comprising a cell, wherein the cell is transfected or transduced with the protein.

5 . The prepolymerization solution of claim 1 , wherein the protein is a cyan fluorescent protein (CFP) or a green fluorescent protein (GFP).

6 . The prepolymerization solution of claim 1 , further comprising a cryoprotectant.

7 . The prepolymerization solution of claim 4 , further comprising a medium configured to provide nutrition and/or buoyancy for the cell.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 26, 2024
From: MILLER, JORDAN; TA, ANDERSON; GRIGORYAN, BAGRAT
To: WILLIAM MARSH RICE UNIVERSITY
Reel/Frame 068100/0430 →
Continuity (6)
Continuation 17849904 · Jun 27, 2022
Continuation 17071923 · Oct 15, 2020
Division 15709392 · Sep 19, 2017
Continuation PCTUS2016023302 · Mar 18, 2016
Provisional Application 62136004 · Mar 20, 2015
Related Publication 20240352408A1 · Oct 24, 2024
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